##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631873.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8403
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.544567416398905	33.0	31.0	34.0	31.0	34.0
2	32.71867190289183	34.0	31.0	34.0	31.0	34.0
3	32.79852433654647	34.0	31.0	34.0	31.0	34.0
4	36.243484469832204	37.0	37.0	37.0	35.0	37.0
5	36.14792336070451	37.0	35.0	37.0	35.0	37.0
6	36.21575627751994	37.0	36.0	37.0	35.0	37.0
7	36.20623586814233	37.0	36.0	37.0	35.0	37.0
8	36.20635487325955	37.0	36.0	37.0	35.0	37.0
9	38.036891586338214	39.0	38.0	39.0	35.0	39.0
10	37.96072831131739	39.0	38.0	39.0	35.0	39.0
11	38.05355230274902	39.0	38.0	39.0	35.0	39.0
12	37.977984053314294	39.0	38.0	39.0	35.0	39.0
13	38.03879566821373	39.0	38.0	39.0	35.0	39.0
14	39.357610377246225	40.0	39.0	41.0	37.0	41.0
15	39.371057955492084	40.0	39.0	41.0	37.0	41.0
16	39.302987028442224	40.0	39.0	41.0	36.0	41.0
17	39.37486611924313	40.0	39.0	41.0	37.0	41.0
18	39.387242651434015	40.0	39.0	41.0	37.0	41.0
19	39.39462096870165	40.0	39.0	41.0	37.0	41.0
20	39.40759252647864	40.0	39.0	41.0	37.0	41.0
21	39.30953230988933	40.0	39.0	41.0	36.0	41.0
22	39.257765083898605	40.0	39.0	41.0	36.0	41.0
23	39.23860526002618	40.0	39.0	41.0	36.0	41.0
24	39.21075806259669	40.0	39.0	41.0	36.0	41.0
25	39.17410448649292	40.0	39.0	41.0	36.0	41.0
26	39.135546828513625	40.0	39.0	41.0	36.0	41.0
27	38.98119719147923	40.0	39.0	41.0	35.0	41.0
28	39.01808877781745	40.0	39.0	41.0	35.0	41.0
29	38.958467214090206	40.0	39.0	41.0	35.0	41.0
30	38.924669760799716	40.0	38.0	41.0	35.0	41.0
31	38.860168987266455	40.0	38.0	41.0	35.0	41.0
32	38.81197191479234	40.0	38.0	41.0	35.0	41.0
33	38.667737712721646	40.0	38.0	41.0	35.0	41.0
34	38.75818160180888	40.0	38.0	41.0	35.0	41.0
35	38.6508389860764	40.0	38.0	41.0	35.0	41.0
36	38.623467809115795	40.0	38.0	41.0	35.0	41.0
37	38.57646078781388	40.0	38.0	41.0	35.0	41.0
38	38.509341901701774	40.0	38.0	41.0	35.0	41.0
39	38.36344162799001	40.0	38.0	41.0	34.0	41.0
40	38.037724622158755	40.0	37.0	41.0	34.0	41.0
41	38.11733904557896	40.0	37.0	41.0	34.0	41.0
42	38.135665833630846	40.0	37.0	41.0	34.0	41.0
43	37.632512198024514	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	1.0
24	3.0
25	7.0
26	17.0
27	15.0
28	33.0
29	40.0
30	52.0
31	92.0
32	87.0
33	136.0
34	265.0
35	300.0
36	520.0
37	878.0
38	2187.0
39	3767.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.87790074973224	15.47066523860526	14.030703320242772	28.620730691419734
2	21.1710103534452	19.278828989646556	31.845769368082827	27.70439128882542
3	21.98024515054147	18.85041056765441	29.917886469118173	29.251457812685942
4	15.077948351779128	18.183981911222183	35.51112697846007	31.226942758538616
5	16.4703082232536	31.50065452814471	33.8212543139355	18.20778293466619
6	29.620373676068073	35.20171367368797	17.220040461739856	17.957872188504105
7	27.573485659883374	27.656789241937403	21.1710103534452	23.59871474473402
8	26.02641913602285	29.775080328454123	22.0516482208735	22.14685231464953
9	28.03760561704153	12.519338331548258	18.064976794002142	41.37807925740807
10	20.02856122813281	22.313459478757586	28.608830179697726	29.049149113411875
11	39.84291324526954	18.540997262882303	18.93371414970844	22.682375342139714
12	21.016303701059147	25.240985362370584	26.49053909318101	27.252171843389267
13	37.105795549208615	16.82732357491372	19.814352017136738	26.252528858740927
14	22.932286088301797	21.873140545043437	24.277043912888256	30.917529453766512
15	31.488754016422703	23.670117815066046	19.4216351303106	25.419493038200642
16	23.55111269784601	24.62215875282637	23.55111269784601	28.27561585148161
17	26.371533975960965	24.812566940378435	20.540283232178982	28.27561585148161
18	27.0736641675592	20.183267880518862	22.18255384981554	30.56051410210639
19	28.239914316315602	22.777579435915747	23.17029632274188	25.812209925026774
20	31.833868856360827	19.08842080209449	21.694632869213375	27.38307747233131
21	28.16851124598358	21.777936451267404	21.29001547066524	28.76353683208378
22	29.679876234678094	23.027490182077827	20.94490063072712	26.347732952516957
23	28.311317386647627	20.813995001785077	22.32535999047959	28.549327621087706
24	28.073307152207544	20.278471974294895	24.550755682494348	27.097465191003213
25	27.335475425443295	22.158752826371533	23.396406045459955	27.10936570272522
26	27.371176960609304	23.194097346185885	21.62322979888135	27.811495894323457
27	26.288230393906936	21.42092109960728	26.871355468285135	25.419493038200642
28	25.43139354992265	22.337260502201595	21.944543615375462	30.286802332500294
29	27.549684636439366	23.753421397120075	21.301915982387243	27.394977984053316
30	27.0498631441152	20.94490063072712	24.336546471498274	27.668689753659407
31	28.620730691419734	22.503867666309652	22.468166131143636	26.40723551112698
32	26.811852909675117	20.683089372843032	21.96834463881947	30.53671307866238
33	26.347732952516957	21.313816494109247	24.86016898726645	27.47828156610734
34	25.693204807806737	22.218255384981553	24.050934190170178	28.03760561704153
35	24.181839819112223	24.64595977627038	25.62180173747471	25.550398667142687
36	26.72854932762109	22.32535999047959	24.562656194216352	26.383434487682973
37	27.597286683327383	22.84898250624777	23.94382958467214	25.609901225752708
38	26.692847792455076	21.599428775437342	24.764964893490422	26.942758538617163
39	26.216827323574915	20.314173509460907	25.264786385814592	28.20421278114959
40	26.50243960490301	22.52766868975366	25.717005831250745	25.252885874092584
41	23.931929072950137	22.14685231464953	26.58574318695704	27.335475425443295
42	20.694989884565036	22.24205640842556	28.501725574199693	28.561228132809713
43	19.980959181244792	24.634059264548373	27.394977984053316	27.990003570153515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	1.0
11	2.0
12	2.0
13	3.5
14	5.0
15	11.0
16	17.0
17	15.5
18	14.0
19	14.0
20	22.0
21	30.0
22	18.5
23	7.0
24	11.0
25	15.0
26	15.0
27	14.5
28	14.0
29	10.0
30	6.0
31	7.5
32	9.0
33	9.0
34	15.5
35	22.0
36	25.5
37	29.0
38	48.5
39	68.0
40	68.0
41	95.5
42	123.0
43	198.5
44	274.0
45	507.5
46	741.0
47	741.0
48	859.5
49	978.0
50	1116.5
51	1255.0
52	1245.0
53	1235.0
54	1235.0
55	1069.5
56	904.0
57	856.5
58	809.0
59	748.0
60	687.0
61	687.0
62	587.5
63	488.0
64	365.5
65	243.0
66	205.0
67	167.0
68	167.0
69	135.5
70	104.0
71	94.0
72	84.0
73	61.5
74	39.0
75	39.0
76	27.0
77	15.0
78	12.5
79	10.0
80	7.5
81	5.0
82	5.0
83	3.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.50184457931691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57275541795666	49.55373081042485
2	9.462074303405572	11.638700464119957
3	3.3862229102167185	6.247768654052124
4	1.8962848297213624	4.665000595025586
5	1.2577399380804952	3.867666309651315
6	0.7159442724458205	2.641913602284898
7	0.6385448916408669	2.7490182077829344
8	0.42569659442724456	2.0944900630727123
9	0.21284829721362228	1.1781506604784007
>10	1.431888544891641	15.363560633107223
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	47	0.5593240509341901	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	42	0.49982149232416995	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	41	0.4879209806021659	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	37	0.4403189337141497	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	34	0.40461739854813755	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	34	0.40461739854813755	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	34	0.40461739854813755	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	33	0.39271688682613354	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	32	0.38081637510412947	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	30	0.3570153516601214	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	29	0.3451148399381173	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	28	0.3332143282161133	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	27	0.32131381649410923	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	26	0.3094133047721052	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	23	0.27371176960609306	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.27371176960609306	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	20	0.23801023444008093	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	20	0.23801023444008093	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	19	0.22610972271807686	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	19	0.22610972271807686	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	19	0.22610972271807686	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	19	0.22610972271807686	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	19	0.22610972271807686	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	17	0.20230869927406878	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	17	0.20230869927406878	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	16	0.19040818755206473	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	16	0.19040818755206473	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	16	0.19040818755206473	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	16	0.19040818755206473	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	15	0.1785076758300607	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	15	0.1785076758300607	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	15	0.1785076758300607	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	15	0.1785076758300607	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	14	0.16660716410805665	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	14	0.16660716410805665	RNA PCR Primer, Index 27 (95% over 23bp)
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	14	0.16660716410805665	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	13	0.1547066523860526	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	13	0.1547066523860526	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	13	0.1547066523860526	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	13	0.1547066523860526	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	13	0.1547066523860526	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT	13	0.1547066523860526	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	13	0.1547066523860526	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	13	0.1547066523860526	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	13	0.1547066523860526	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	13	0.1547066523860526	No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC	12	0.14280614066404856	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	12	0.14280614066404856	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	12	0.14280614066404856	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	12	0.14280614066404856	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	12	0.14280614066404856	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	12	0.14280614066404856	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	12	0.14280614066404856	No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG	11	0.13090562894204452	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	11	0.13090562894204452	No Hit
CTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCA	11	0.13090562894204452	No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT	11	0.13090562894204452	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	11	0.13090562894204452	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG	11	0.13090562894204452	No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC	11	0.13090562894204452	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	11	0.13090562894204452	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	11	0.13090562894204452	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	11	0.13090562894204452	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	11	0.13090562894204452	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	11	0.13090562894204452	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	10	0.11900511722004047	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	10	0.11900511722004047	No Hit
CTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTA	10	0.11900511722004047	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	10	0.11900511722004047	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	10	0.11900511722004047	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	10	0.11900511722004047	No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC	10	0.11900511722004047	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	10	0.11900511722004047	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	10	0.11900511722004047	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	9	0.10710460549803642	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	9	0.10710460549803642	No Hit
TTAGTGGACCATGAAGTGCGATTGCCTCGGTGATAACGACATT	9	0.10710460549803642	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	9	0.10710460549803642	No Hit
TCGCAGATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCA	9	0.10710460549803642	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	9	0.10710460549803642	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	9	0.10710460549803642	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	9	0.10710460549803642	No Hit
GGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAAC	9	0.10710460549803642	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	9	0.10710460549803642	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	9	0.10710460549803642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.011900511722004046	0.0
25	0.0	0.0	0.0	0.02380102344400809	0.0
26	0.0	0.0	0.0	0.02380102344400809	0.0
27	0.0	0.0	0.0	0.03570153516601214	0.0
28	0.0	0.0	0.0	0.08330358205402832	0.0
29	0.0	0.0	0.0	0.23801023444008093	0.0
30	0.0	0.0	0.0	0.4522194454361538	0.0
31	0.0	0.0	0.0	0.9639414494823277	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE