ERR1631872.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631872.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58904
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	159	0.26993073475485535	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	131	0.2223957625967676	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	129	0.2190004074426185	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	124	0.2105120195572457	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	112	0.19013988863235096	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	109	0.18504685590112727	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	109	0.18504685590112727	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	108	0.1833491783240527	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	106	0.17995382316990358	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	88	0.14939562678256146	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	80	0.13581420616596496	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	80	0.13581420616596496	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	71	0.12053510797229391	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	68	0.11544207524107021	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	65	0.11034904250984652	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	64	0.10865136493277197	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	63	0.1069536873556974	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	63	0.1069536873556974	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	61	0.10355833220154827	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	61	0.10355833220154827	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	60	0.10186065462447372	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	60	0.10186065462447372	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	59	0.10016297704739917	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	59	0.10016297704739917	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCCC	20	0.0018277959	37.0	29
ATAGTGA	30	3.5585387E-4	30.833334	30
TAGTGAA	30	3.5585387E-4	30.833334	31
ACGGAGG	45	3.924055E-6	28.777779	31
CCGATAG	35	8.7694667E-4	26.428572	27
ACCGATA	35	8.7694667E-4	26.428572	26
GGTGCAA	35	8.7694667E-4	26.428572	37
CGATAGT	35	8.7694667E-4	26.428572	28
TACGGAG	50	8.9014375E-6	25.900002	30
ATACGGA	50	8.9014375E-6	25.900002	29
GGTATCA	115	0.0	25.73913	1
CGGAGGG	45	1.3025104E-4	24.666668	32
TAATACG	55	1.8632456E-5	23.545454	27
AATACGG	55	1.8632456E-5	23.545454	28
GGGTGCA	40	0.001909635	23.125002	36
ACTGACC	40	0.001909635	23.125002	22
GACCGAT	40	0.001909635	23.125002	25
AGGGTGC	40	0.001909635	23.125002	35
TGACCGA	40	0.001909635	23.125002	24
AGTGAAC	40	0.001909635	23.125002	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content