##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631872.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58904
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.415014260491645	33.0	31.0	34.0	31.0	34.0
2	32.58853388564444	34.0	31.0	34.0	31.0	34.0
3	32.67336683417086	34.0	31.0	34.0	31.0	34.0
4	36.1423502648377	37.0	35.0	37.0	35.0	37.0
5	36.05872266739101	37.0	35.0	37.0	35.0	37.0
6	36.10763275838653	37.0	35.0	37.0	35.0	37.0
7	36.098906695640366	37.0	35.0	37.0	35.0	37.0
8	36.08700597582507	37.0	35.0	37.0	35.0	37.0
9	37.906950292000545	39.0	38.0	39.0	35.0	39.0
10	37.81403639820725	39.0	38.0	39.0	35.0	39.0
11	37.889701887817466	39.0	38.0	39.0	35.0	39.0
12	37.813000814885235	39.0	38.0	39.0	35.0	39.0
13	37.878463262257235	39.0	38.0	39.0	35.0	39.0
14	39.21625016976776	40.0	39.0	41.0	36.0	41.0
15	39.23118973244601	40.0	39.0	41.0	36.0	41.0
16	39.15569401059351	40.0	39.0	41.0	36.0	41.0
17	39.122419530082844	40.0	39.0	41.0	36.0	41.0
18	39.15068586174114	40.0	39.0	41.0	36.0	41.0
19	39.161313323373626	40.0	39.0	41.0	36.0	41.0
20	39.13975281814478	40.0	39.0	41.0	36.0	41.0
21	39.09914437050116	40.0	39.0	41.0	36.0	41.0
22	39.07768572592693	40.0	39.0	41.0	36.0	41.0
23	39.012715605052286	40.0	39.0	41.0	36.0	41.0
24	39.035260763275836	40.0	39.0	41.0	36.0	41.0
25	38.994397663995656	40.0	39.0	41.0	35.0	41.0
26	38.887664674724974	40.0	38.0	41.0	35.0	41.0
27	38.76201955724569	40.0	38.0	41.0	35.0	41.0
28	38.76682398478881	40.0	38.0	41.0	35.0	41.0
29	38.752393725383676	40.0	38.0	41.0	35.0	41.0
30	38.718083661551	40.0	38.0	41.0	35.0	41.0
31	38.61299741953008	40.0	38.0	41.0	35.0	41.0
32	38.58294852641586	40.0	38.0	41.0	35.0	41.0
33	38.516026076327584	40.0	38.0	41.0	34.0	41.0
34	38.49565394540269	40.0	38.0	41.0	34.0	41.0
35	38.42124473719951	40.0	38.0	41.0	34.0	41.0
36	38.38304699171533	40.0	38.0	41.0	34.0	41.0
37	38.32165897052832	40.0	38.0	41.0	34.0	41.0
38	38.27592353660193	40.0	38.0	41.0	34.0	41.0
39	38.19200733396713	40.0	37.0	41.0	34.0	41.0
40	37.8759846529947	40.0	37.0	41.0	33.0	41.0
41	37.94769455385033	40.0	37.0	41.0	33.0	41.0
42	37.92309520575852	40.0	37.0	41.0	33.0	41.0
43	37.40431889175608	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	2.0
18	0.0
19	1.0
20	5.0
21	3.0
22	19.0
23	19.0
24	49.0
25	62.0
26	98.0
27	138.0
28	225.0
29	347.0
30	444.0
31	676.0
32	895.0
33	1205.0
34	1737.0
35	2549.0
36	4014.0
37	7100.0
38	15856.0
39	23459.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.131196523156326	16.223006926524512	12.469441803612657	28.176354746706505
2	21.52655167730545	19.728711123183484	31.522477251120467	27.222259948390604
3	21.521458644574224	19.445198967812033	28.616053239168814	30.417289148444926
4	16.034564715469237	15.900448186880348	34.052016840961564	34.01297025668885
5	17.387613744397665	32.31019964688306	33.36106206709222	16.941124541627055
6	34.09445878038843	33.72606274616325	15.489610213228303	16.68986826022002
7	30.524242835800624	27.4157951921771	19.670990085562952	22.388971886459323
8	27.278283308434066	30.702498981393454	20.219339942958033	21.79987776721445
9	28.273122368599758	12.340418307754991	17.430055683824527	41.95640363982073
10	19.465571098736927	23.256485128344426	29.67200869210919	27.605935080809452
11	39.85637647697949	19.207524107021595	18.589569468966456	22.346529947032458
12	22.90167051473584	23.517927475213906	26.439630585359232	27.140771424691025
13	35.15211191090588	17.39270677712889	20.86615509982344	26.589026212141793
14	24.499185114763	19.800013581420618	22.723414369143015	32.97738693467337
15	29.634659785413554	23.811625696047805	19.643827244329756	26.90988727420888
16	25.809792204264564	23.611299741953008	22.51290234958577	28.066005704196662
17	27.371655575173165	23.438136629091403	21.433179410566343	27.757028385169093
18	26.61449137579791	21.748947439902214	23.051066141518405	28.585495042781474
19	28.432704060844767	21.92211055276382	23.27685725926932	26.368328127122098
20	29.758590248539996	20.927271492598127	22.76246095341573	26.551677305446148
21	27.371655575173165	21.589365747657205	22.363506722803205	28.675471954366426
22	28.901263072117345	22.80490289284259	20.978201819910364	27.315632215129703
23	28.60247181855222	21.83383131875594	21.82534293087057	27.73835393182127
24	27.407306804291732	21.93738965095749	22.229390194214314	28.425913350536465
25	28.894472361809044	21.7031101453212	22.241273937253837	27.16114355561592
26	28.15258726062746	22.826972701344562	22.173366834170853	26.847073203857125
27	27.407306804291732	21.540133097922045	23.59602064375934	27.456539454026892
28	26.32079315496401	22.98655439358957	21.794784734483226	28.897867716963194
29	27.096631807687082	23.29553171261714	22.232785549368465	27.375050930327312
30	27.16114355561592	21.898343066684774	23.438136629091403	27.502376748607904
31	28.341029471682738	22.407646339807144	22.346529947032458	26.90479424147766
32	26.08651364932772	21.779505636289556	23.030694010593507	29.103286703789216
33	26.03049028928426	21.636900719815294	24.46523156322151	27.867377427678935
34	26.468491104169495	21.708203178052425	23.59432296618226	28.228982751595815
35	26.196862691837563	23.64864864864865	23.799741953008287	26.354746706505498
36	26.566956403639818	21.735366019285618	24.73516229797637	26.96251527909819
37	27.149259812576393	21.738761374439765	24.1256960478066	26.98628276517724
38	26.132350943908733	21.4162026347956	25.07809316854543	27.37335325275024
39	25.477047399157954	20.777875865815567	25.692652451446424	28.052424283580063
40	25.110349042509846	21.942482683688713	26.804631264430263	26.142537009371182
41	23.877835121553716	20.567363846258317	27.59065598261578	27.96414504957219
42	22.329553171261715	21.34320249898139	28.40723889718865	27.920005432568246
43	22.095273665625427	21.984924623115578	27.998098601113675	27.921703110145323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	3.0
7	4.0
8	2.5
9	1.0
10	3.5
11	6.0
12	6.0
13	21.5
14	37.0
15	58.0
16	79.0
17	78.5
18	78.0
19	78.0
20	92.5
21	107.0
22	84.0
23	61.0
24	81.0
25	101.0
26	101.0
27	112.5
28	124.0
29	178.0
30	232.0
31	303.0
32	374.0
33	374.0
34	499.0
35	624.0
36	699.0
37	774.0
38	1052.0
39	1330.0
40	1330.0
41	1564.5
42	1799.0
43	2171.0
44	2543.0
45	3353.5
46	4164.0
47	4164.0
48	4609.0
49	5054.0
50	5795.0
51	6536.0
52	6778.5
53	7021.0
54	7021.0
55	6504.5
56	5988.0
57	5692.0
58	5396.0
59	4951.5
60	4507.0
61	4507.0
62	4230.5
63	3954.0
64	3286.0
65	2618.0
66	2224.5
67	1831.0
68	1831.0
69	1532.5
70	1234.0
71	1091.0
72	948.0
73	734.0
74	520.0
75	520.0
76	436.0
77	352.0
78	291.5
79	231.0
80	190.5
81	150.0
82	150.0
83	108.0
84	66.0
85	50.5
86	35.0
87	24.5
88	14.0
89	14.0
90	11.0
91	8.0
92	4.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	58904.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.19414640771424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.64843168936422	58.01813119652316
2	5.850067964402041	7.744805106614152
3	2.056884922161524	4.084612250441396
4	1.1566771819137749	3.06261034904251
5	0.7181144366648714	2.376748607904387
6	0.48985663358210874	1.945538503327448
7	0.3205868020825319	1.4854678799402417
8	0.2846810802492883	1.507537688442211
9	0.2282578030827627	1.359839739236724
>10	1.1541124874971147	13.77665353796007
>50	0.06924674924982688	2.792679614287654
>100	0.023082249749942295	1.8453755262800489
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	159	0.26993073475485535	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	131	0.2223957625967676	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	129	0.2190004074426185	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	124	0.2105120195572457	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	112	0.19013988863235096	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	109	0.18504685590112727	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	109	0.18504685590112727	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	108	0.1833491783240527	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	106	0.17995382316990358	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	88	0.14939562678256146	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	80	0.13581420616596496	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	80	0.13581420616596496	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	71	0.12053510797229391	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	68	0.11544207524107021	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	65	0.11034904250984652	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	64	0.10865136493277197	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	63	0.1069536873556974	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	63	0.1069536873556974	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	61	0.10355833220154827	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	61	0.10355833220154827	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	60	0.10186065462447372	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	60	0.10186065462447372	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	59	0.10016297704739917	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	59	0.10016297704739917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.001697677577074562
10	0.0	0.0	0.0	0.0	0.001697677577074562
11	0.0	0.0	0.0	0.0	0.001697677577074562
12	0.0	0.0	0.0	0.0	0.001697677577074562
13	0.0	0.0	0.0	0.0	0.001697677577074562
14	0.0	0.0	0.0	0.0	0.001697677577074562
15	0.0	0.0	0.0	0.0	0.001697677577074562
16	0.0	0.0	0.0	0.0	0.001697677577074562
17	0.0	0.0	0.0	0.001697677577074562	0.001697677577074562
18	0.0	0.0	0.0	0.001697677577074562	0.001697677577074562
19	0.0	0.0	0.0	0.001697677577074562	0.001697677577074562
20	0.0	0.0	0.0	0.001697677577074562	0.001697677577074562
21	0.0	0.0	0.0	0.001697677577074562	0.001697677577074562
22	0.0	0.0	0.0	0.005093032731223686	0.001697677577074562
23	0.0	0.0	0.0	0.006790710308298248	0.001697677577074562
24	0.0	0.0	0.0	0.011883743039521935	0.001697677577074562
25	0.0	0.0	0.0	0.011883743039521935	0.001697677577074562
26	0.0	0.0	0.0	0.015279098193671058	0.001697677577074562
27	0.0	0.0	0.0	0.04244193942686405	0.001697677577074562
28	0.0	0.0	0.0	0.11883743039521934	0.001697677577074562
29	0.0	0.0	0.0	0.2903028656797501	0.001697677577074562
30	0.0	0.0	0.0	0.44139617003938614	0.001697677577074562
31	0.0	0.0	0.0	0.8878853728099959	0.001697677577074562
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCCC	20	0.0018277959	37.0	29
ATAGTGA	30	3.5585387E-4	30.833334	30
TAGTGAA	30	3.5585387E-4	30.833334	31
ACGGAGG	45	3.924055E-6	28.777779	31
CCGATAG	35	8.7694667E-4	26.428572	27
ACCGATA	35	8.7694667E-4	26.428572	26
GGTGCAA	35	8.7694667E-4	26.428572	37
CGATAGT	35	8.7694667E-4	26.428572	28
TACGGAG	50	8.9014375E-6	25.900002	30
ATACGGA	50	8.9014375E-6	25.900002	29
GGTATCA	115	0.0	25.73913	1
CGGAGGG	45	1.3025104E-4	24.666668	32
TAATACG	55	1.8632456E-5	23.545454	27
AATACGG	55	1.8632456E-5	23.545454	28
GGGTGCA	40	0.001909635	23.125002	36
ACTGACC	40	0.001909635	23.125002	22
GACCGAT	40	0.001909635	23.125002	25
AGGGTGC	40	0.001909635	23.125002	35
TGACCGA	40	0.001909635	23.125002	24
AGTGAAC	40	0.001909635	23.125002	32
>>END_MODULE