FastQCFastQC Report
Fri 10 Feb 2017
ERR1631871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83103
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3240.3898776217465073No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2840.3417445820247163No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2820.3393379300386268No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2630.31647473617077604No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2540.30564480223337304No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2470.2972215202820596No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2400.2887982383307462No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2310.27796830439334325No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2210.26593504446289545No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2200.26473171846985066No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2200.26473171846985066No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2060.24788515456722382No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2040.24547850258113424No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2020.24307185059504471No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1900.22863193867850737No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1810.2178020047411044No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1730.20817539679674624No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1690.20336209282456713No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1660.1997521148454328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1530.18410887693585068No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1500.18049889895671636No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1500.18049889895671636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.17929557296367157No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1490.17929557296367157No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1480.1780922469706268No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1370.16485566104713428No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1350.16244900906104473No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1340.16124568306799997No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1320.15883903108191041No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1300.15643237909582086No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1250.15041574913059697No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1240.14921242313755217No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1230.1480090971445074No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1220.14680577115146262No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1200.1443991191653731No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1200.1443991191653731No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1180.14199246717928354No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1170.14078914118623875No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1160.139585815193194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1160.139585815193194No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1150.1383824892001492No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1140.13717916320710444No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1140.13717916320710444No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1120.13477251122101488No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1090.13116253324188057No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1080.12995920724883578No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1080.12995920724883578No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1060.12755255526274623No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1060.12755255526274623No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1050.12634922926970146No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1050.12634922926970146No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1040.12514590327665667No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1030.12394257728361191No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1030.12394257728361191No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1030.12394257728361191No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1020.12273925129056712No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1020.12273925129056712No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC980.11792594731838803No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA980.11792594731838803No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA980.11792594731838803No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA980.11792594731838803No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT970.11672262132534324No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT970.11672262132534324No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG960.11551929533229847No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA950.11431596933925368No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG950.11431596933925368No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA930.11190931735316413No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG910.1095026653670746No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG910.1095026653670746No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC890.10709601338098505No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA870.1046893613948955No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG860.10348603540185071No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC850.10228270940880595No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG850.10228270940880595No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC850.10228270940880595No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT850.10228270940880595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAGG200.001832128437.037
GGTATCA2150.034.4186061
CTTATAC601.2350938E-930.83333237
CTCAAAG250.00546741329.636
GTATCAA2800.026.4285722
GCGGTAA1300.025.61538523
CGCGGTA1300.025.61538522
CGTGCCA1300.025.61538510
CCGCGGT1300.025.61538521
AACTCCG1350.024.6666685
ATCTAAG451.3088384E-424.66666632
GAGCTGT451.3088384E-424.66666634
GTAACGT601.3126391E-624.66666626
GGAGCTG451.3088384E-424.66666633
TGCGGGT601.3126391E-624.66666621
CCGTGCC1400.023.7857159
CCAGCAG1400.023.78571514
GCAGCCG1400.023.78571517
TAATACG1400.023.78571527
TCCGTGC1400.023.7857158
Produced by FastQC (version 0.11.5)