##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631871.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 83103 Sequences flagged as poor quality 0 Sequence length 43 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54194192748758 33.0 31.0 34.0 31.0 34.0 2 32.713644513435135 34.0 31.0 34.0 31.0 34.0 3 32.79869559462354 34.0 31.0 34.0 31.0 34.0 4 36.23485313406255 37.0 35.0 37.0 35.0 37.0 5 36.14829789538284 37.0 35.0 37.0 35.0 37.0 6 36.2221099117962 37.0 36.0 37.0 35.0 37.0 7 36.2146011575996 37.0 36.0 37.0 35.0 37.0 8 36.19716496396039 37.0 36.0 37.0 35.0 37.0 9 38.02395822052152 39.0 38.0 39.0 35.0 39.0 10 37.95699312900858 39.0 38.0 39.0 35.0 39.0 11 38.030010950266536 39.0 38.0 39.0 35.0 39.0 12 37.9653803111801 39.0 38.0 39.0 35.0 39.0 13 38.03659314344849 39.0 38.0 39.0 35.0 39.0 14 39.36664139682081 40.0 39.0 41.0 37.0 41.0 15 39.383644393102536 40.0 39.0 41.0 37.0 41.0 16 39.30421284430165 40.0 39.0 41.0 36.0 41.0 17 39.294477937017916 40.0 39.0 41.0 36.0 41.0 18 39.341503916826106 40.0 39.0 41.0 36.0 41.0 19 39.33919353091946 40.0 39.0 41.0 36.0 41.0 20 39.3497226333586 40.0 39.0 41.0 37.0 41.0 21 39.30509127227657 40.0 39.0 41.0 36.0 41.0 22 39.28445423149586 40.0 39.0 41.0 36.0 41.0 23 39.233625741549645 40.0 39.0 41.0 36.0 41.0 24 39.238848176359454 40.0 39.0 41.0 36.0 41.0 25 39.17817647979014 40.0 39.0 41.0 36.0 41.0 26 39.101452414473606 40.0 39.0 41.0 36.0 41.0 27 38.94045942986414 40.0 38.0 41.0 35.0 41.0 28 38.93782414593937 40.0 38.0 41.0 35.0 41.0 29 38.92582698578872 40.0 38.0 41.0 35.0 41.0 30 38.88440850510812 40.0 38.0 41.0 35.0 41.0 31 38.80128274550859 40.0 38.0 41.0 35.0 41.0 32 38.763149344789 40.0 38.0 41.0 35.0 41.0 33 38.701575153724896 40.0 38.0 41.0 35.0 41.0 34 38.67931362285357 40.0 38.0 41.0 35.0 41.0 35 38.61236056460056 40.0 38.0 41.0 35.0 41.0 36 38.55994368434352 40.0 38.0 41.0 35.0 41.0 37 38.52491486468599 40.0 38.0 41.0 35.0 41.0 38 38.460224059299904 40.0 38.0 41.0 34.0 41.0 39 38.35874757830644 40.0 38.0 41.0 34.0 41.0 40 38.00990337292276 40.0 37.0 41.0 34.0 41.0 41 38.08617017436194 40.0 37.0 41.0 34.0 41.0 42 38.05436626836576 40.0 37.0 41.0 34.0 41.0 43 37.565310518272504 39.0 36.0 41.0 33.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 5.0 22 14.0 23 11.0 24 35.0 25 69.0 26 102.0 27 172.0 28 297.0 29 412.0 30 576.0 31 861.0 32 1149.0 33 1481.0 34 2167.0 35 3322.0 36 5024.0 37 9408.0 38 21233.0 39 36763.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.771019096783505 16.2268510162088 13.294345571158683 27.707784315849004 2 21.650241266861606 19.50230436927668 32.127600688302465 26.719853675559246 3 21.906549703380144 19.39881833387483 29.63430923071369 29.060322732031334 4 15.481992226514086 16.834530642696414 35.98787047399011 31.69560665679939 5 16.966896501931338 32.430838838549754 33.66545130741369 16.93681335210522 6 30.604189981107783 34.91209703620808 16.605898704017903 17.877814278666236 7 28.652395220389153 27.847370131042197 20.35907247632456 23.14116217224408 8 26.465951891026794 29.647545816637184 21.68393439466686 22.202567897669155 9 28.296210726447903 12.344921362646355 17.978893662081994 41.37997424882375 10 20.76218668399456 22.221821113557873 28.275754184566143 28.740238017881424 11 39.448636029986886 19.468611241471425 18.410887693585067 22.67186503495662 12 21.776590496131305 24.17602252626259 26.78964658315584 27.25774039445026 13 36.56787360263769 17.80922469706268 20.303719480644503 25.31918221965513 14 23.544276379914084 21.309700010829935 24.109839596645127 31.036184012610857 15 31.349048770802497 23.136348868271902 20.107577343778203 25.407025017147394 16 24.011166865215454 24.172412548283457 23.54066640193495 28.275754184566143 17 26.467155217019844 23.451620278449635 21.699577632576442 28.381646871954082 18 26.468358543012886 20.491438335559486 23.439587018519187 29.60061610290844 19 28.09164530763029 22.10991179620471 23.539463075941903 26.258979820223093 20 30.92788467323683 19.130476637425843 23.21817503579895 26.72346365353838 21 27.682514469995066 21.582855011251098 21.949869439129756 28.784761079624083 22 28.47309964742548 23.02925285489092 20.892145891243395 27.605501606440203 23 28.717374824013575 21.668291156757277 22.243480981432683 27.370853037796472 24 27.725834205744675 20.650277366641397 23.46846684235226 28.155421585261664 25 28.21438455892086 22.03891556261507 23.42514710660265 26.321552771861423 26 28.276957510559185 22.89327701767686 22.46489296415292 26.364872507611036 27 26.954502244202978 21.424015980169187 25.22291614021154 26.398565635416286 28 25.382958497286502 23.423943780609605 22.08945525432295 29.10364246778095 29 27.878656606861362 23.728385256849933 21.685137720659903 26.707820415628795 30 27.057988279604828 21.64903794086856 24.35291144724017 26.94006233228644 31 28.46708301746026 22.007629086795905 22.63576525516528 26.889522640578562 32 25.245779334079394 21.905346377387097 22.851160607920292 29.997713680613213 33 25.75959953310952 21.484182279821425 25.5093077265562 27.246910460512858 34 25.333622131571666 21.91136300735232 24.22896886995656 28.526045991119453 35 25.127853386761007 24.310795037483604 24.837851822437216 25.723499753318173 36 26.05321107541244 22.401116686521547 25.1868163604202 26.358855877645816 37 27.038735063716114 22.43721646631289 24.4154843988785 26.1085640710925 38 26.012297991648918 21.581651685258052 24.920881315957306 27.485169007135724 39 25.174783100489755 20.090730779875578 26.57906453437301 28.155421585261664 40 25.185613034427156 21.983562566935007 27.005041935910857 25.825782462726977 41 23.451620278449635 20.55280796120477 28.21438455892086 27.78118720142474 42 21.10152461403319 21.042561640373993 30.19505914347256 27.66085460212026 43 20.75496672803629 22.50339939593035 28.76430453774232 27.977329338291035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 2.0 4 4.0 5 4.0 6 5.5 7 7.0 8 13.0 9 19.0 10 24.5 11 30.0 12 30.0 13 56.5 14 83.0 15 161.5 16 240.0 17 224.5 18 209.0 19 209.0 20 241.0 21 273.0 22 191.5 23 110.0 24 98.5 25 87.0 26 87.0 27 75.5 28 64.0 29 64.0 30 64.0 31 87.0 32 110.0 33 110.0 34 228.0 35 346.0 36 348.5 37 351.0 38 651.0 39 951.0 40 951.0 41 1327.0 42 1703.0 43 2265.0 44 2827.0 45 4901.0 46 6975.0 47 6975.0 48 8104.0 49 9233.0 50 10457.0 51 11681.0 52 11742.0 53 11803.0 54 11803.0 55 10660.5 56 9518.0 57 8920.0 58 8322.0 59 7377.5 60 6433.0 61 6433.0 62 5662.5 63 4892.0 64 3795.5 65 2699.0 66 2212.0 67 1725.0 68 1725.0 69 1429.5 70 1134.0 71 897.5 72 661.0 73 464.0 74 267.0 75 267.0 76 198.0 77 129.0 78 102.0 79 75.0 80 56.0 81 37.0 82 37.0 83 31.0 84 25.0 85 16.0 86 7.0 87 6.5 88 6.0 89 6.0 90 4.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 83103.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.62315439875817 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29373461694239 29.467046917680467 2 9.318627909150388 7.198296090393849 3 4.05645387419385 4.7001913288328945 4 2.190235847587002 3.3837526924419095 5 1.3895379630495062 2.68341696448985 6 0.9814001308533506 2.274286126854626 7 0.7321556531763094 1.979471258558656 8 0.6418045300183818 1.9830812365377906 9 0.510951179237935 1.776109165734089 >10 3.377262672523912 25.04001058926874 >50 0.33024893292207996 8.848056026858236 >100 0.17758669034489202 10.666281602348892 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA 324 0.3898776217465073 No Hit ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA 284 0.3417445820247163 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 282 0.3393379300386268 No Hit TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA 263 0.31647473617077604 No Hit GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA 254 0.30564480223337304 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 247 0.2972215202820596 No Hit CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA 240 0.2887982383307462 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 231 0.27796830439334325 No Hit TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT 221 0.26593504446289545 No Hit CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC 220 0.26473171846985066 No Hit GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG 220 0.26473171846985066 No Hit CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA 206 0.24788515456722382 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 204 0.24547850258113424 No Hit GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT 202 0.24307185059504471 No Hit GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC 190 0.22863193867850737 No Hit GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 181 0.2178020047411044 No Hit CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA 173 0.20817539679674624 No Hit CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA 169 0.20336209282456713 No Hit GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC 166 0.1997521148454328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.18410887693585068 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA 150 0.18049889895671636 No Hit GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC 150 0.18049889895671636 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.17929557296367157 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 149 0.17929557296367157 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 148 0.1780922469706268 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 137 0.16485566104713428 No Hit CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA 135 0.16244900906104473 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 134 0.16124568306799997 No Hit GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC 132 0.15883903108191041 No Hit GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT 130 0.15643237909582086 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 125 0.15041574913059697 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC 124 0.14921242313755217 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 123 0.1480090971445074 No Hit GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT 122 0.14680577115146262 No Hit GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA 120 0.1443991191653731 No Hit CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT 120 0.1443991191653731 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT 118 0.14199246717928354 No Hit CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA 117 0.14078914118623875 No Hit GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC 116 0.139585815193194 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.139585815193194 No Hit TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC 115 0.1383824892001492 No Hit CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC 114 0.13717916320710444 No Hit CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC 114 0.13717916320710444 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 112 0.13477251122101488 No Hit CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG 109 0.13116253324188057 No Hit CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC 108 0.12995920724883578 No Hit GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG 108 0.12995920724883578 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 106 0.12755255526274623 No Hit GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC 106 0.12755255526274623 No Hit CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT 105 0.12634922926970146 No Hit ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG 105 0.12634922926970146 No Hit TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA 104 0.12514590327665667 No Hit GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC 103 0.12394257728361191 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 103 0.12394257728361191 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 103 0.12394257728361191 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 102 0.12273925129056712 No Hit ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC 102 0.12273925129056712 No Hit CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC 98 0.11792594731838803 No Hit AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA 98 0.11792594731838803 No Hit GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA 98 0.11792594731838803 No Hit GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA 98 0.11792594731838803 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 97 0.11672262132534324 No Hit CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT 97 0.11672262132534324 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 96 0.11551929533229847 No Hit ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA 95 0.11431596933925368 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 95 0.11431596933925368 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 93 0.11190931735316413 No Hit GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG 91 0.1095026653670746 No Hit GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG 91 0.1095026653670746 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 89 0.10709601338098505 No Hit CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA 87 0.1046893613948955 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 86 0.10348603540185071 No Hit GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC 85 0.10228270940880595 No Hit AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG 85 0.10228270940880595 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC 85 0.10228270940880595 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 85 0.10228270940880595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0012033259930447758 0.0 16 0.0 0.0 0.0 0.004813303972179103 0.0 17 0.0 0.0 0.0 0.004813303972179103 0.0 18 0.0 0.0 0.0 0.007219955958268655 0.0 19 0.0 0.0 0.0 0.007219955958268655 0.0 20 0.0 0.0 0.0 0.007219955958268655 0.0 21 0.0 0.0 0.0 0.009626607944358207 0.0 22 0.0 0.0 0.0 0.010829933937402982 0.0 23 0.0 0.0 0.0 0.01443991191653731 0.0 24 0.0 0.0 0.0 0.01684656390262686 0.0 25 0.0 0.0 0.0 0.02286319386785074 0.0 26 0.0 0.0 0.0 0.02887982383307462 0.0 27 0.0 0.0 0.0 0.051743017700925355 0.0 28 0.0 0.0 0.0 0.1540257271097313 0.0 29 0.0 0.0 0.0 0.30564480223337304 0.0 30 0.0 0.0 0.0 0.5198368289953431 0.0 31 0.0012033259930447758 0.0 0.0 1.099839957642925 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAAAGG 20 0.0018321284 37.0 37 GGTATCA 215 0.0 34.418606 1 CTTATAC 60 1.2350938E-9 30.833332 37 CTCAAAG 25 0.005467413 29.6 36 GTATCAA 280 0.0 26.428572 2 GCGGTAA 130 0.0 25.615385 23 CGCGGTA 130 0.0 25.615385 22 CGTGCCA 130 0.0 25.615385 10 CCGCGGT 130 0.0 25.615385 21 AACTCCG 135 0.0 24.666668 5 ATCTAAG 45 1.3088384E-4 24.666666 32 GAGCTGT 45 1.3088384E-4 24.666666 34 GTAACGT 60 1.3126391E-6 24.666666 26 GGAGCTG 45 1.3088384E-4 24.666666 33 TGCGGGT 60 1.3126391E-6 24.666666 21 CCGTGCC 140 0.0 23.785715 9 CCAGCAG 140 0.0 23.785715 14 GCAGCCG 140 0.0 23.785715 17 TAATACG 140 0.0 23.785715 27 TCCGTGC 140 0.0 23.785715 8 >>END_MODULE