FastQCFastQC Report
Fri 10 Feb 2017
ERR1631870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409201
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11360.27761417982849507No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9920.24242364999108018No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8650.21138755770391568No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7750.1893934765555314No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7080.1730201050339564No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6420.15689111219180796No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6260.15298105332098405No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6220.1520035386032781No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6190.15127040256499863No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6100.1490709944501602No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6080.14858223709130722No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6070.1483378584118807No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5560.13587454576112962No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5540.13538578840227664No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5490.13416389500514417No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5420.13245324424915872No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4850.11852365952184868No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4810.11754614480414272No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4550.11119229913905392No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4300.10508283215339159No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4260.10410531743568563No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4180.1021502880002737No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4140.10117277328256774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGTC303.5954633E-430.8333345
TAGGACC303.5954633E-430.8333344
GGTATCA4900.029.4489781
TTCGTAC451.3211789E-424.6666661
CTTAGGA806.937589E-720.81252
TAGAAGT450.003821662420.5555554
TAGGACA450.003821662420.5555554
CTTATAC2400.020.04166837
GTTTCCG656.8882546E-519.92307716
TTCTGCG1950.019.92307518
ATACGGC851.241302E-619.58823629
GCAGTCG951.6695412E-719.4736849
TCTTATA4950.019.06060637
AGGACAG801.6125277E-518.55
TGCGGGT2000.018.521
CGTTAGA609.222963E-418.51
TAGATAC500.0070273318.55
TAAGGTA500.0070273318.55
GGCGACT500.0070273318.512
GTAACGT2000.018.526
Produced by FastQC (version 0.11.5)