##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631870.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409201
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.281443593735105	33.0	31.0	34.0	31.0	34.0
2	32.46762104686939	34.0	31.0	34.0	31.0	34.0
3	32.557689252958816	34.0	31.0	34.0	31.0	34.0
4	36.08104330145821	37.0	35.0	37.0	35.0	37.0
5	35.947407264400624	37.0	35.0	37.0	35.0	37.0
6	35.98886610736533	37.0	35.0	37.0	35.0	37.0
7	35.99092133205931	37.0	35.0	37.0	35.0	37.0
8	35.966786493679145	37.0	35.0	37.0	35.0	37.0
9	37.76799910068646	39.0	37.0	39.0	35.0	39.0
10	37.65095881974873	39.0	37.0	39.0	35.0	39.0
11	37.74226358195605	39.0	37.0	39.0	35.0	39.0
12	37.660521357474686	39.0	37.0	39.0	35.0	39.0
13	37.725240163147205	39.0	37.0	39.0	35.0	39.0
14	39.00733869174318	40.0	38.0	41.0	36.0	41.0
15	39.01139049024807	40.0	38.0	41.0	36.0	41.0
16	38.94675966090014	40.0	38.0	41.0	36.0	41.0
17	38.92040830789759	40.0	38.0	41.0	36.0	41.0
18	38.94696249520407	40.0	38.0	41.0	36.0	41.0
19	38.952106666405996	40.0	38.0	41.0	36.0	41.0
20	38.948296802793735	40.0	38.0	41.0	36.0	41.0
21	38.89622214999475	40.0	38.0	41.0	35.0	41.0
22	38.86587520558356	40.0	38.0	41.0	35.0	41.0
23	38.81390807940352	40.0	38.0	41.0	35.0	41.0
24	38.82334598400297	40.0	38.0	41.0	35.0	41.0
25	38.76881288168895	40.0	38.0	41.0	35.0	41.0
26	38.6795633441756	40.0	38.0	41.0	35.0	41.0
27	38.54409691080912	40.0	38.0	41.0	35.0	41.0
28	38.545844218367016	40.0	38.0	41.0	35.0	41.0
29	38.51609600172043	40.0	38.0	41.0	35.0	41.0
30	38.49048511611653	40.0	38.0	41.0	34.0	41.0
31	38.40414124110156	40.0	38.0	41.0	34.0	41.0
32	38.35433931002124	40.0	38.0	41.0	34.0	41.0
33	38.29258970530375	40.0	38.0	41.0	34.0	41.0
34	38.26434930510923	40.0	38.0	41.0	34.0	41.0
35	38.1907277841452	40.0	38.0	41.0	34.0	41.0
36	38.14217951569033	40.0	38.0	41.0	33.0	41.0
37	38.08320360898434	40.0	37.0	41.0	33.0	41.0
38	38.03967487860489	40.0	37.0	41.0	33.0	41.0
39	37.95884663038458	40.0	37.0	41.0	33.0	41.0
40	37.64336353039216	40.0	37.0	41.0	33.0	41.0
41	37.71594399818182	40.0	37.0	41.0	33.0	41.0
42	37.704348229843035	40.0	37.0	41.0	33.0	41.0
43	37.20874093660573	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	8.0
19	14.0
20	24.0
21	57.0
22	94.0
23	168.0
24	338.0
25	507.0
26	778.0
27	1244.0
28	1841.0
29	2671.0
30	3693.0
31	5049.0
32	6906.0
33	9374.0
34	13538.0
35	19792.0
36	30980.0
37	54806.0
38	122461.0
39	134856.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.93146888692843	16.953282127854038	12.70573630074218	27.409512684475356
2	21.36578356357878	20.015591359747408	32.080566763033325	26.538058313640484
3	21.535137988421337	20.027077157680456	29.41879418671998	29.018990667178237
4	15.927380431621623	16.049081013976014	34.44810740931718	33.575431145085176
5	16.929088638590812	33.083985620758504	33.39825660250097	16.58866913814971
6	33.49331990879787	34.188088494407396	15.659785777649615	16.658805819145115
7	29.714248010146605	27.989912048113275	20.11041028736489	22.185429654375234
8	27.172220986752233	30.747725445441237	20.564954631098164	21.515098936708366
9	28.31273628363567	12.80324339383335	17.604795687205065	41.27922463532592
10	19.274635203726287	23.389239029230133	30.14068880574583	27.195436961297748
11	38.934411206228724	19.4647618163201	19.27781212655883	22.32301485089235
12	22.585721931275828	23.9571750802173	26.960100293010036	26.497002695496835
13	35.08422511186434	17.79394478508117	20.955716139501128	26.16611396355336
14	23.941534844734004	20.278542818810315	23.329855010129496	32.450067326326185
15	29.408041524825208	24.56274544783615	19.99139787048419	26.037815156854453
16	25.318608703302285	24.27364547007461	22.69007162739094	27.71767419923216
17	26.657803866559465	23.758495213843563	21.893397132460578	27.690303787136394
18	26.290013954022594	21.626535614526844	23.73552361797747	28.347926813473084
19	28.024125063232987	22.535135544634542	23.57838812710624	25.862351265026234
20	29.750171676022298	20.980154007443772	22.958643796080654	26.311030520453272
21	27.367723930293426	22.42736454700746	22.065439722776826	28.13947179992229
22	28.129696652745228	23.053462723698136	21.60258650394305	27.21425411961359
23	27.93248305844805	22.42809768304574	22.477950933648742	27.16146832485747
24	27.86625643632347	21.941295353628167	22.69911363852972	27.49333457151864
25	27.970361753759153	22.313484082394716	23.036844973497132	26.679309190349
26	27.84695052064878	22.91441125510446	22.357472244691483	26.881165979555277
27	27.030970110043718	21.936896537398493	24.16709636584466	26.865036986713132
28	26.31005300573557	23.1499923020716	22.232594739504545	28.307359952688287
29	26.98624881170867	23.597205285422078	22.44349353984961	26.97305236301964
30	26.738937588129062	22.624578141304642	23.635817116771467	27.000667153794833
31	27.944213235060523	22.526093533495764	22.803707713324258	26.725985518119455
32	25.64094418146583	22.488459216864083	22.962065097592628	28.908531504077455
33	25.987717527572023	22.344520174681882	24.53073184083128	27.137030456914818
34	26.18957431677831	22.21719888270068	23.86651059015007	27.726716210370945
35	25.869927004088456	23.96890525682977	24.13361648676323	26.027551252318542
36	26.265087328721094	22.68102961625216	24.64778922827657	26.406093826750176
37	27.112103831613314	22.470130815907098	23.896324789040104	26.521440563439487
38	25.990650071725142	22.111382914509008	25.065432391416444	26.832534622349407
39	25.60062169936046	21.208648072707543	25.858685584834834	27.33204464309716
40	25.28855012573283	22.063973450700267	26.55443168516206	26.093044738404842
41	23.76240527271439	21.34501137582753	27.396560614465752	27.49602273699233
42	22.61064855657733	21.59501076488083	28.44250136241114	27.351839316130704
43	21.902439143599356	22.242614265361034	28.04025405607513	27.81469253496448
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	8.5
4	14.0
5	14.0
6	22.0
7	30.0
8	31.0
9	32.0
10	53.5
11	75.0
12	75.0
13	143.0
14	211.0
15	417.0
16	623.0
17	608.5
18	594.0
19	594.0
20	732.5
21	871.0
22	814.5
23	758.0
24	910.5
25	1063.0
26	1063.0
27	1239.0
28	1415.0
29	1950.0
30	2485.0
31	2984.0
32	3483.0
33	3483.0
34	4226.5
35	4970.0
36	5690.5
37	6411.0
38	8054.5
39	9698.0
40	9698.0
41	11740.5
42	13783.0
43	15785.0
44	17787.0
45	23968.5
46	30150.0
47	30150.0
48	33691.5
49	37233.0
50	41752.5
51	46272.0
52	47400.5
53	48529.0
54	48529.0
55	44791.0
56	41053.0
57	39067.5
58	37082.0
59	33858.5
60	30635.0
61	30635.0
62	27832.0
63	25029.0
64	20447.5
65	15866.0
66	13623.0
67	11380.0
68	11380.0
69	9697.0
70	8014.0
71	6736.5
72	5459.0
73	4340.0
74	3221.0
75	3221.0
76	2568.5
77	1916.0
78	1612.5
79	1309.0
80	1011.0
81	713.0
82	713.0
83	597.5
84	482.0
85	375.0
86	268.0
87	214.0
88	160.0
89	160.0
90	130.5
91	101.0
92	60.5
93	20.0
94	11.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	409201.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.0842374678453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.47575886198295	44.6863243838551
2	5.717829684795601	5.84181938837587
3	1.9390981338682678	2.9717204863194677
4	0.995202603426951	2.0335666448832095
5	0.6738200334792067	1.721079130042163
6	0.4389304545222187	1.3453456542409377
7	0.33258321454940154	1.189283193690268
8	0.25163543603556227	1.0283683495812368
9	0.1972792217386768	0.9070072749693245
>10	1.6741178842301732	17.268230956320775
>50	0.1725383943389281	6.121673655317161
>100	0.1235163435796757	12.182214049217025
>500	0.007209125111654224	2.4244616726660784
>1k	4.806083407769483E-4	0.27890516052138375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1136	0.27761417982849507	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	992	0.24242364999108018	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	865	0.21138755770391568	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	775	0.1893934765555314	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	708	0.1730201050339564	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	642	0.15689111219180796	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	626	0.15298105332098405	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	622	0.1520035386032781	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	619	0.15127040256499863	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	610	0.1490709944501602	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	608	0.14858223709130722	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	607	0.1483378584118807	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	556	0.13587454576112962	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	554	0.13538578840227664	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	549	0.13416389500514417	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	542	0.13245324424915872	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	485	0.11852365952184868	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	481	0.11754614480414272	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	455	0.11119229913905392	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	430	0.10508283215339159	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	426	0.10410531743568563	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	418	0.1021502880002737	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	414	0.10117277328256774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.4437867942649213E-4	0.0	0.0	0.0	0.0
9	2.4437867942649213E-4	0.0	0.0	0.0	0.0
10	2.4437867942649213E-4	0.0	0.0	0.0	0.0
11	2.4437867942649213E-4	0.0	0.0	0.0	0.0
12	2.4437867942649213E-4	0.0	0.0	0.0	0.0
13	2.4437867942649213E-4	0.0	0.0	0.0	0.0
14	2.4437867942649213E-4	0.0	0.0	0.0	0.0
15	4.887573588529843E-4	0.0	0.0	4.887573588529843E-4	0.0
16	7.331360382794764E-4	0.0	0.0	9.775147177059685E-4	0.0
17	7.331360382794764E-4	0.0	0.0	0.0014662720765589528	0.0
18	7.331360382794764E-4	0.0	0.0	0.0017106507559854448	0.0
19	7.331360382794764E-4	0.0	0.0	0.001955029435411937	0.0
20	7.331360382794764E-4	0.0	0.0	0.0036656801913973816	0.0
21	7.331360382794764E-4	0.0	0.0	0.005865088306235811	0.0
22	7.331360382794764E-4	0.0	0.0	0.009530768497633192	0.0
23	7.331360382794764E-4	0.0	0.0	0.01026390453591267	0.0
24	7.331360382794764E-4	0.0	0.0	0.01270769133017759	0.0
25	7.331360382794764E-4	0.0	0.0	0.014418342086163034	0.0
26	7.331360382794764E-4	0.0	0.0	0.015884614162721987	0.0
27	7.331360382794764E-4	0.0	0.0	0.028103548134046592	0.0
28	7.331360382794764E-4	0.0	0.0	0.09090886874665506	0.0
29	7.331360382794764E-4	0.0	0.0	0.19208164202922282	0.0
30	7.331360382794764E-4	0.0	0.0	0.3347987908142942	0.0
31	7.331360382794764E-4	0.0	0.0	0.6903697693798402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGTC	30	3.5954633E-4	30.833334	5
TAGGACC	30	3.5954633E-4	30.833334	4
GGTATCA	490	0.0	29.448978	1
TTCGTAC	45	1.3211789E-4	24.666666	1
CTTAGGA	80	6.937589E-7	20.8125	2
TAGAAGT	45	0.0038216624	20.555555	4
TAGGACA	45	0.0038216624	20.555555	4
CTTATAC	240	0.0	20.041668	37
GTTTCCG	65	6.8882546E-5	19.923077	16
TTCTGCG	195	0.0	19.923075	18
ATACGGC	85	1.241302E-6	19.588236	29
GCAGTCG	95	1.6695412E-7	19.473684	9
TCTTATA	495	0.0	19.060606	37
AGGACAG	80	1.6125277E-5	18.5	5
TGCGGGT	200	0.0	18.5	21
CGTTAGA	60	9.222963E-4	18.5	1
TAGATAC	50	0.00702733	18.5	5
TAAGGTA	50	0.00702733	18.5	5
GGCGACT	50	0.00702733	18.5	12
GTAACGT	200	0.0	18.5	26
>>END_MODULE