FastQCFastQC Report
Fri 10 Feb 2017
ERR1631869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230867
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7710.3339585129100305No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6950.3010391264234386No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6290.27245123815876676No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6020.2607561929595828No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5670.24559594918286287No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5470.2369329527390229No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5400.2339009039836789No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5360.23216830469491093No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5270.22826995629518293No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4770.20661246518558304No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4640.20098151749708706No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4460.1931848206976311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.16546323207734323No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3680.15939913456665525No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3620.15680023563350326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3580.1550676363447353No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3460.1498698384784313No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3370.14597149007870333No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3340.14467204061212732No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3320.14380574096774335No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3320.14380574096774335No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3320.14380574096774335No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3290.14250629150116734No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3260.14120684203459136No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3220.13947424274582335No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3170.13730849363486336No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3160.13687534381267136No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3140.13600904416828735No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2880.12474714879129542No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2880.12474714879129542No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2860.12388084914691141No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2820.12214824985814343No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2780.12041565056937545No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2770.11998250074718345No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2690.11651730216964745No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2680.11608415234745546No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2640.11435155305868747No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2530.10958690501457549No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2510.1087206053701915No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2490.1078543057258075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2480.1074211559036155No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2440.10568855661484751No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2360.10222335803731153No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2360.10222335803731153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACC200.001838891237.04
GGTATCA4100.030.2317071
TATGGCT250.00548736129.634
ATCCTCT358.847853E-426.428576
GGGTACT957.070412E-921.4210531
AGAACAG450.003816691220.5555555
CCTCCAC450.003816691220.5555553
CTACGCC450.003816691220.5555553
CACTTCC555.1260006E-420.18181830
GTATCAA6200.019.3951632
GCGGTAA2050.018.95121823
CGCGGTA2150.018.93023322
CGGTTGG500.00701827918.522
ATCAATT500.00701827918.528
AGGGGCG500.00701827918.517
GGTAATA2150.018.06976725
GGTACTT1054.756348E-717.6190472
TAATACG2000.017.57527
ATCTAAG852.707884E-517.41176432
CATCTAA852.707884E-517.41176431
Produced by FastQC (version 0.11.5)