##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631869.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230867
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.523366267158146	33.0	31.0	34.0	31.0	34.0
2	32.69751848466866	34.0	31.0	34.0	31.0	34.0
3	32.77504797134281	34.0	31.0	34.0	31.0	34.0
4	36.230379395929255	37.0	37.0	37.0	35.0	37.0
5	36.145928175096486	37.0	35.0	37.0	35.0	37.0
6	36.206889681071786	37.0	36.0	37.0	35.0	37.0
7	36.19961709555718	37.0	36.0	37.0	35.0	37.0
8	36.17908579398528	37.0	36.0	37.0	35.0	37.0
9	38.013046472644426	39.0	38.0	39.0	35.0	39.0
10	37.9349885431872	39.0	38.0	39.0	35.0	39.0
11	38.01873806130803	39.0	38.0	39.0	35.0	39.0
12	37.94039858446638	39.0	38.0	39.0	35.0	39.0
13	38.003984978364166	39.0	38.0	39.0	35.0	39.0
14	39.34061602567712	40.0	39.0	41.0	36.0	41.0
15	39.361810046476975	40.0	39.0	41.0	36.0	41.0
16	39.29145352085833	40.0	39.0	41.0	36.0	41.0
17	39.28036055391199	40.0	39.0	41.0	36.0	41.0
18	39.313098017473266	40.0	39.0	41.0	36.0	41.0
19	39.33043700485561	40.0	39.0	41.0	36.0	41.0
20	39.32960968869522	40.0	39.0	41.0	36.0	41.0
21	39.27752775407486	40.0	39.0	41.0	36.0	41.0
22	39.250941017988715	40.0	39.0	41.0	36.0	41.0
23	39.179908778647444	40.0	39.0	41.0	36.0	41.0
24	39.19030870587827	40.0	39.0	41.0	36.0	41.0
25	39.15230414047915	40.0	39.0	41.0	36.0	41.0
26	39.09155487791672	40.0	39.0	41.0	36.0	41.0
27	38.97100061940424	40.0	39.0	41.0	35.0	41.0
28	38.97445715498534	40.0	39.0	41.0	35.0	41.0
29	38.96734916640317	40.0	38.0	41.0	35.0	41.0
30	38.912707316333645	40.0	38.0	41.0	35.0	41.0
31	38.83004500426652	40.0	38.0	41.0	35.0	41.0
32	38.78831968189477	40.0	38.0	41.0	35.0	41.0
33	38.72190048815985	40.0	38.0	41.0	35.0	41.0
34	38.691870210987275	40.0	38.0	41.0	35.0	41.0
35	38.62256623943656	40.0	38.0	41.0	35.0	41.0
36	38.56949239172337	40.0	38.0	41.0	35.0	41.0
37	38.52214478465957	40.0	38.0	41.0	35.0	41.0
38	38.48339953306449	40.0	38.0	41.0	34.0	41.0
39	38.41283076403297	40.0	38.0	41.0	34.0	41.0
40	38.094517622700515	40.0	37.0	41.0	34.0	41.0
41	38.18758852499491	40.0	37.0	41.0	34.0	41.0
42	38.16178579008693	40.0	37.0	41.0	34.0	41.0
43	37.69434782797022	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	1.0
19	7.0
20	8.0
21	13.0
22	43.0
23	58.0
24	131.0
25	201.0
26	325.0
27	512.0
28	799.0
29	1174.0
30	1596.0
31	2226.0
32	3056.0
33	4212.0
34	6124.0
35	9144.0
36	14448.0
37	25044.0
38	57966.0
39	103775.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.32090337726916	16.358336184902996	13.636855852070672	27.68390458575717
2	20.640888476915283	19.826566811194326	32.759121052380806	26.77342365950959
3	20.943660202627488	19.7087500595581	30.229959240601733	29.117630497212684
4	15.213088055027354	16.79321860638376	34.98724373773644	33.00644960085244
5	16.44366669987482	32.68158723420844	34.15992757734973	16.71481848856701
6	32.18346493868764	34.496051839370715	16.535061312357332	16.785421909584304
7	29.282660579467834	28.31327127740214	20.711491897932575	21.69257624519745
8	26.624853270497734	30.64751566919482	21.419691857216492	21.307939203090957
9	28.589187714138443	12.816036939016836	18.30404518618945	40.29073016065527
10	19.39385013882452	23.481485010850403	29.8691454387158	27.25551941160928
11	38.51091754126835	19.55238297374679	19.182906175417013	22.753793309567847
12	22.200227836806473	24.448708563805134	26.950581936786115	26.400481662602278
13	35.34242659193389	17.81631848640126	21.216544590608446	25.62471033105641
14	23.633520598439794	21.31530275006822	24.32872606305795	30.722450588434036
15	29.931085863289255	24.43701351860595	20.588477348430047	25.043423269674747
16	24.16196338151403	24.845906950755197	23.3840263008572	27.608103366873564
17	26.354567781449926	24.252058544529966	22.28815725071145	27.105216423308658
18	26.450727041976553	21.259859572827644	23.777326339407537	28.51208704578827
19	27.737615163708973	22.675826341573288	23.65821013830474	25.928348356413
20	30.23602333811242	20.46979429714944	23.43340538058709	25.860776984151045
21	27.312695188138626	22.41420384896932	22.15604655494289	28.117054407949166
22	28.256095500872796	23.099880017499252	21.68131434982046	26.962710131807494
23	27.954190074804973	22.133955914011096	22.889369203913944	27.022484807269986
24	27.301866442583826	21.664421506754973	23.83840046433661	27.195311586324593
25	27.349512923024943	22.17770404605249	24.115183200717297	26.35759983020527
26	27.482923068260078	23.241953159178227	22.946111830621092	26.3290119419406
27	26.144490117686807	22.09107408161409	25.393408326005883	26.37102747469322
28	25.4250282630259	23.421277185565714	22.660232947974375	28.49346160343401
29	26.854855826081685	23.790753983895492	22.927053238444646	26.42733695157818
30	26.547319452325365	22.301151745377208	24.180588823868288	26.970939978429136
31	27.633659206382895	22.60565607037818	23.53476243898002	26.2259222842589
32	25.26086448041513	22.632511359354087	23.125869006830772	28.98075515340001
33	25.140015680023563	21.953765587979227	25.9231505585467	26.983068173450516
34	25.548909112172808	21.953765587979227	24.793062672447775	27.70426262740019
35	24.871029640442334	24.262454140262577	25.63164072821148	25.234875491083606
36	25.495631684043197	22.889802353736133	25.513390826753064	26.101175135467606
37	26.223323385325752	22.894567001780246	24.981915994923483	25.90019361797052
38	25.450150952713035	21.99578112073185	25.79017356313375	26.763894363421365
39	24.88575673439686	20.672941563757487	26.84489338017127	27.59640832167438
40	24.38676813923168	22.127025516856026	27.74671130997501	25.73949503393729
41	22.975132868707956	20.953622648537902	28.691844221997947	27.379400260756192
42	21.320500547934525	21.311404401668494	29.82669675614098	27.541398294256
43	20.58457900003032	22.12572606738945	29.3818518887498	27.907843043830432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	8.5
4	14.0
5	14.0
6	18.0
7	22.0
8	24.5
9	27.0
10	49.0
11	71.0
12	71.0
13	145.0
14	219.0
15	433.0
16	647.0
17	601.0
18	555.0
19	555.0
20	666.0
21	777.0
22	637.5
23	498.0
24	566.0
25	634.0
26	634.0
27	705.0
28	776.0
29	1071.0
30	1366.0
31	1553.0
32	1740.0
33	1740.0
34	2173.5
35	2607.0
36	2898.0
37	3189.0
38	4004.0
39	4819.0
40	4819.0
41	5847.0
42	6875.0
43	8351.5
44	9828.0
45	14820.0
46	19812.0
47	19812.0
48	22079.0
49	24346.0
50	27059.0
51	29772.0
52	29687.0
53	29602.0
54	29602.0
55	26104.0
56	22606.0
57	21164.0
58	19722.0
59	17778.0
60	15834.0
61	15834.0
62	13973.0
63	12112.0
64	9799.0
65	7486.0
66	6377.5
67	5269.0
68	5269.0
69	4451.0
70	3633.0
71	3070.5
72	2508.0
73	1903.5
74	1299.0
75	1299.0
76	1064.0
77	829.0
78	679.0
79	529.0
80	446.5
81	364.0
82	364.0
83	291.5
84	219.0
85	174.5
86	130.0
87	101.0
88	72.0
89	72.0
90	55.0
91	38.0
92	27.0
93	16.0
94	9.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	230867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.31039411648241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.78564778104476	38.801463700449624
2	7.145465384834384	6.6181863623472585
3	2.5170868181293287	3.4970184771891573
4	1.4273095345643754	2.6439706828755716
5	0.9135364007716952	2.1153115379745016
6	0.6599017432463996	1.8336185884736727
7	0.4452548576889478	1.443394955929742
8	0.39633617890084716	1.468358771801513
9	0.30948158781780405	1.2898992873273543
>10	2.032595131543692	18.19636356935912
>50	0.21264379307202214	6.798554961693775
>100	0.14575583938270065	12.79080731521387
>500	0.00898494900304319	2.5030517893648483
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	771	0.3339585129100305	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	695	0.3010391264234386	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	629	0.27245123815876676	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	602	0.2607561929595828	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	567	0.24559594918286287	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	547	0.2369329527390229	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	540	0.2339009039836789	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	536	0.23216830469491093	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	527	0.22826995629518293	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	477	0.20661246518558304	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	464	0.20098151749708706	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	446	0.1931848206976311	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.16546323207734323	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	368	0.15939913456665525	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	362	0.15680023563350326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.1550676363447353	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	346	0.1498698384784313	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	337	0.14597149007870333	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	334	0.14467204061212732	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	332	0.14380574096774335	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	332	0.14380574096774335	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	332	0.14380574096774335	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	329	0.14250629150116734	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	326	0.14120684203459136	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	322	0.13947424274582335	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	317	0.13730849363486336	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	316	0.13687534381267136	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	314	0.13600904416828735	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	288	0.12474714879129542	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	288	0.12474714879129542	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	286	0.12388084914691141	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	282	0.12214824985814343	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	278	0.12041565056937545	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	277	0.11998250074718345	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	269	0.11651730216964745	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	268	0.11608415234745546	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	264	0.11435155305868747	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	253	0.10958690501457549	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	251	0.1087206053701915	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	249	0.1078543057258075	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	248	0.1074211559036155	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	244	0.10568855661484751	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	236	0.10222335803731153	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	236	0.10222335803731153	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.33149822191998E-4	0.0	0.0	0.0	0.0
6	4.33149822191998E-4	0.0	0.0	0.0	0.0
7	4.33149822191998E-4	0.0	0.0	0.0	0.0
8	8.66299644383996E-4	0.0	0.0	0.0	0.0
9	8.66299644383996E-4	0.0	0.0	0.0	0.0
10	8.66299644383996E-4	0.0	0.0	0.0	0.0
11	8.66299644383996E-4	0.0	0.0	0.0	0.0
12	0.001732599288767992	0.0	0.0	0.0	0.0
13	0.001732599288767992	0.0	0.0	0.0	0.0
14	0.001732599288767992	0.0	0.0	4.33149822191998E-4	0.0
15	0.00216574911095999	0.0	0.0	4.33149822191998E-4	0.0
16	0.002598898933151988	0.0	0.0	0.001732599288767992	0.0
17	0.002598898933151988	0.0	0.0	0.00216574911095999	0.0
18	0.002598898933151988	0.0	0.0	0.002598898933151988	0.0
19	0.002598898933151988	0.0	0.0	0.003465198577535984	0.0
20	0.002598898933151988	0.0	0.0	0.0064972473328799695	0.0
21	0.002598898933151988	0.0	0.0	0.011261895376991947	0.0
22	0.002598898933151988	0.0	0.0	0.01949174199863991	0.0
23	0.002598898933151988	0.0	0.0	0.0220906409317919	0.0
24	0.002598898933151988	0.0	0.0	0.026422139153711877	0.0
25	0.002598898933151988	0.0	0.0	0.029021038086863866	0.0
26	0.002598898933151988	0.0	0.0	0.033785686130975844	0.0
27	0.002598898933151988	0.0	0.0	0.05154482884084776	0.0
28	0.002598898933151988	0.0	0.0	0.14250629150116734	0.0
29	0.002598898933151988	0.0	0.0	0.29064353069083065	0.0
30	0.002598898933151988	0.0	0.0	0.5076515916090216	0.0
31	0.002598898933151988	0.0	0.0	1.0477894198824431	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAACC	20	0.0018388912	37.0	4
GGTATCA	410	0.0	30.231707	1
TATGGCT	25	0.005487361	29.6	34
ATCCTCT	35	8.847853E-4	26.42857	6
GGGTACT	95	7.070412E-9	21.421053	1
AGAACAG	45	0.0038166912	20.555555	5
CCTCCAC	45	0.0038166912	20.555555	3
CTACGCC	45	0.0038166912	20.555555	3
CACTTCC	55	5.1260006E-4	20.181818	30
GTATCAA	620	0.0	19.395163	2
GCGGTAA	205	0.0	18.951218	23
CGCGGTA	215	0.0	18.930233	22
CGGTTGG	50	0.007018279	18.5	22
ATCAATT	50	0.007018279	18.5	28
AGGGGCG	50	0.007018279	18.5	17
GGTAATA	215	0.0	18.069767	25
GGTACTT	105	4.756348E-7	17.619047	2
TAATACG	200	0.0	17.575	27
ATCTAAG	85	2.707884E-5	17.411764	32
CATCTAA	85	2.707884E-5	17.411764	31
>>END_MODULE