FastQCFastQC Report
Fri 10 Feb 2017
ERR1631868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276030
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6470.23439481215809876No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6350.23004745860957143No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5690.20613701409267107No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5640.20432561678078467No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5360.1941817918342209No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5130.1858493641995435No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4990.18077745172626164No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4970.18005289280150708No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4960.1796906133391298No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4480.16230119914502047No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4250.15396877151034308No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4240.15360649204796578No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3610.1307828859181973No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3490.12643553236966998No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3410.12353729667065173No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3190.11556714849835163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3180.11520486903597435No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3150.11411803064884252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.10723472086367423No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2910.10542332355178785No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2830.10252508785276962No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2800.1014382494656378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGG200.001839514137.01
TTAGAAC303.592457E-430.8333343
TATGCGG250.005489229.5999985
CGTTAGA250.005489229.5999981
GGTATCA3600.028.7777791
AACACCT451.3196561E-424.66666836
CGCGCAA400.001927491423.12521
CATACTG400.001927491423.1255
TACTCAC400.001927491423.1252
TGCGGGT1250.022.221
CTTATAC2750.021.52727337
CACATAC450.00381855620.5555573
TTTATAA450.00381855620.5555572
CCGGTAT450.00381855620.55555723
ATACGGC450.00381855620.55555729
GCGGGTA1351.8189894E-1220.55555522
GTAACGT1351.8189894E-1220.55555526
TAATACG2100.020.26190627
GGTAACG1403.6379788E-1219.82142825
GCGGTAA2250.019.73333423
Produced by FastQC (version 0.11.5)