##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631868.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276030
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5580154331051	34.0	31.0	34.0	31.0	34.0
2	32.72896062022244	34.0	31.0	34.0	31.0	34.0
3	32.820432561678075	34.0	31.0	34.0	31.0	34.0
4	36.25312103756838	37.0	37.0	37.0	35.0	37.0
5	36.16761946165272	37.0	35.0	37.0	35.0	37.0
6	36.22670361917183	37.0	36.0	37.0	35.0	37.0
7	36.21735680904249	37.0	36.0	37.0	35.0	37.0
8	36.194127449914866	37.0	36.0	37.0	35.0	37.0
9	38.0276890193095	39.0	38.0	39.0	35.0	39.0
10	37.95604101003514	39.0	38.0	39.0	35.0	39.0
11	38.04215846103684	39.0	38.0	39.0	35.0	39.0
12	37.97728145491432	39.0	38.0	39.0	35.0	39.0
13	38.01662138173387	39.0	38.0	39.0	35.0	39.0
14	39.39418179183422	40.0	39.0	41.0	37.0	41.0
15	39.40800999891316	40.0	39.0	41.0	37.0	41.0
16	39.345915299061694	40.0	39.0	41.0	36.0	41.0
17	39.31664674129624	40.0	39.0	41.0	36.0	41.0
18	39.34261855595406	40.0	39.0	41.0	36.0	41.0
19	39.35765315364272	40.0	39.0	41.0	36.0	41.0
20	39.362898960257944	40.0	39.0	41.0	36.0	41.0
21	39.32230916929319	40.0	39.0	41.0	36.0	41.0
22	39.30731804514002	40.0	39.0	41.0	36.0	41.0
23	39.23271383545267	40.0	39.0	41.0	36.0	41.0
24	39.25006702170054	40.0	39.0	41.0	36.0	41.0
25	39.19635184581386	40.0	39.0	41.0	36.0	41.0
26	39.12083831467594	40.0	39.0	41.0	36.0	41.0
27	39.01459623953918	40.0	39.0	41.0	35.0	41.0
28	39.007187624533564	40.0	39.0	41.0	35.0	41.0
29	38.9915117921965	40.0	39.0	41.0	35.0	41.0
30	38.952606600731805	40.0	38.0	41.0	35.0	41.0
31	38.865579828279536	40.0	38.0	41.0	35.0	41.0
32	38.8177842988081	40.0	38.0	41.0	35.0	41.0
33	38.757910372061005	40.0	38.0	41.0	35.0	41.0
34	38.73519182697533	40.0	38.0	41.0	35.0	41.0
35	38.6734376698185	40.0	38.0	41.0	35.0	41.0
36	38.619610187298484	40.0	38.0	41.0	35.0	41.0
37	38.580962214252075	40.0	38.0	41.0	35.0	41.0
38	38.54378871861754	40.0	38.0	41.0	35.0	41.0
39	38.476027967974495	40.0	38.0	41.0	34.0	41.0
40	38.14980618048763	40.0	38.0	41.0	34.0	41.0
41	38.24128536753251	40.0	37.0	41.0	34.0	41.0
42	38.243299641343334	40.0	37.0	41.0	34.0	41.0
43	37.777245226968084	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	3.0
19	5.0
20	12.0
21	27.0
22	43.0
23	97.0
24	152.0
25	245.0
26	397.0
27	609.0
28	903.0
29	1384.0
30	1918.0
31	2702.0
32	3586.0
33	4803.0
34	7090.0
35	10696.0
36	16235.0
37	29375.0
38	67005.0
39	128740.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.40408651233562	16.461616490961127	13.221751258921133	27.912545737782125
2	20.476035213563744	19.646777524182156	32.912002318588556	26.965184943665545
3	20.804622685939933	20.089845306669567	29.359852189979353	29.74567981741115
4	15.620403579321088	16.246784769771402	33.82712024055357	34.305691410353944
5	16.576096801072346	33.10256131579901	33.73292758033547	16.588414302793176
6	33.682570735065035	33.587653515922185	15.980509364924103	16.74926638408869
7	30.205050175705537	27.712929754012244	20.454298445821106	21.62772162446111
8	26.933304350976346	31.123066333369557	20.949896750353222	20.993732565300874
9	28.101655617143063	13.209071477737927	18.072673260152882	40.61659964496613
10	19.0895917110459	24.051733507227475	30.170995906242077	26.68767887548455
11	38.28460674564359	19.901097706771004	19.233778937072056	22.58051661051335
12	22.467123138789262	24.021302032387783	26.857225663877117	26.654349164945838
13	34.258957359707274	17.869072202296852	21.763576422852587	26.108394015143283
14	23.910444516900338	20.53907184001739	23.440930333659384	32.10955330942289
15	29.11241531717567	24.42886642756222	20.377132920334745	26.081585334927365
16	25.305582726515237	24.44118392928305	22.71745824729196	27.535775096909752
17	26.69238850849545	24.30351773357968	22.07731043727131	26.926783320653552
18	26.18700865847915	21.886026881136107	23.78908089700395	28.137883563380793
19	27.755678730572765	22.704053907184	23.752128391841467	25.788138970401768
20	29.328333876752527	21.061841104227803	23.554323805383472	26.0555012136362
21	27.12096511248777	22.69789515632359	22.257363330072817	27.92377640111582
22	27.870521320146363	23.121399847842625	21.68713545629098	27.32094337572003
23	27.308263594536825	22.710212658044416	22.874325254501322	27.10719849291744
24	27.496286635510632	22.003043147483968	23.396732239249356	27.103937977756043
25	27.58576966271782	22.434517987175305	23.570988660652826	26.408723689454046
26	27.408252726152956	23.42535231677716	22.759482664927724	26.40691229214216
27	26.618845777632867	22.145056696735864	24.461109299713797	26.774988225917472
28	25.727638300184765	23.332608774408577	22.514944027823063	28.4248088975836
29	26.850704633554322	23.51737130022099	22.894250624932074	26.737673441292614
30	26.351121254936054	22.71600912944245	24.002825779806543	26.93004383581495
31	27.784661087562945	22.723254718689997	23.23370648117958	26.258377712567476
32	25.350867659312392	22.780494873745607	23.311234286128318	28.55740318081368
33	25.687063000398506	22.237800239104445	24.949824294460747	27.1253124660363
34	26.055863493098574	22.149766329746768	24.37452450820563	27.419845668949026
35	25.604825562438865	23.836177227112994	24.840778176285188	25.718219034162953
36	25.86385537803862	22.815635981596202	25.158134985327678	26.162373655037495
37	26.52682679418904	22.317864000289823	24.659638445096547	26.495670760424588
38	25.493243488026664	21.99760895554831	25.572220410824908	26.936927145600116
39	25.09220012317502	20.98467557874144	26.521030322791	27.40209397529254
40	24.61652718907365	22.068978009636634	27.364054631742928	25.95044016954679
41	23.2445748650509	21.170524942940986	28.276998876933668	27.30790131507445
42	22.14541897619824	21.40021012208818	28.794696228670798	27.659674673042783
43	21.470130058326994	22.264246639857987	28.58167590479296	27.683947397022063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	8.5
4	14.0
5	14.0
6	18.0
7	22.0
8	28.5
9	35.0
10	54.5
11	74.0
12	74.0
13	148.0
14	222.0
15	407.5
16	593.0
17	583.0
18	573.0
19	573.0
20	647.5
21	722.0
22	667.0
23	612.0
24	672.5
25	733.0
26	733.0
27	837.5
28	942.0
29	1372.0
30	1802.0
31	2095.5
32	2389.0
33	2389.0
34	3036.5
35	3684.0
36	4100.5
37	4517.0
38	5758.5
39	7000.0
40	7000.0
41	8386.5
42	9773.0
43	11039.0
44	12305.0
45	17084.0
46	21863.0
47	21863.0
48	24043.5
49	26224.0
50	29410.5
51	32597.0
52	33354.5
53	34112.0
54	34112.0
55	30352.0
56	26592.0
57	24746.5
58	22901.0
59	20630.5
60	18360.0
61	18360.0
62	16686.5
63	15013.0
64	12538.5
65	10064.0
66	8776.0
67	7488.0
68	7488.0
69	6343.0
70	5198.0
71	4432.5
72	3667.0
73	2959.0
74	2251.0
75	2251.0
76	1854.5
77	1458.0
78	1231.0
79	1004.0
80	770.0
81	536.0
82	536.0
83	447.0
84	358.0
85	266.5
86	175.0
87	131.5
88	88.0
89	88.0
90	68.5
91	49.0
92	33.0
93	17.0
94	8.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	276030.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.04655939913959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.45651816219647	45.862208261337464
2	6.3357387005517	6.721782786324866
3	2.0884747107866852	3.323591933980404
4	1.1019970881696595	2.3382861598108287
5	0.7075185658596297	1.876571281493345
6	0.4963571032683774	1.5798021937026507
7	0.35914206564151996	1.3335875642447805
8	0.28321934717406694	1.2019049538283744
9	0.21903145206150115	1.0456978438854199
>10	1.6784097102828541	17.86717208356772
>50	0.17261609173042325	6.276132695023266
>100	0.0968833400226091	9.319555786681802
>500	0.0040936622544764404	1.2537064561190585
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	647	0.23439481215809876	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	635	0.23004745860957143	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	569	0.20613701409267107	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	564	0.20432561678078467	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	536	0.1941817918342209	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	513	0.1858493641995435	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	499	0.18077745172626164	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	497	0.18005289280150708	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	496	0.1796906133391298	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	448	0.16230119914502047	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	425	0.15396877151034308	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	424	0.15360649204796578	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	361	0.1307828859181973	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	349	0.12643553236966998	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	341	0.12353729667065173	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	319	0.11556714849835163	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	318	0.11520486903597435	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	315	0.11411803064884252	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	296	0.10723472086367423	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	291	0.10542332355178785	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	283	0.10252508785276962	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	280	0.1014382494656378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.622794623772778E-4	0.0	0.0	0.0	0.0
9	3.622794623772778E-4	0.0	0.0	0.0	0.0
10	3.622794623772778E-4	0.0	0.0	0.0	0.0
11	3.622794623772778E-4	0.0	0.0	0.0	0.0
12	3.622794623772778E-4	0.0	0.0	0.0	0.0
13	3.622794623772778E-4	0.0	0.0	0.0	0.0
14	3.622794623772778E-4	0.0	0.0	0.0	0.0
15	3.622794623772778E-4	0.0	0.0	3.622794623772778E-4	0.0
16	3.622794623772778E-4	0.0	0.0	3.622794623772778E-4	0.0
17	3.622794623772778E-4	0.0	0.0	7.245589247545556E-4	0.0
18	3.622794623772778E-4	0.0	0.0	0.0010868383871318334	0.0
19	3.622794623772778E-4	0.0	0.0	0.001811397311886389	0.0
20	3.622794623772778E-4	0.0	0.0	0.001811397311886389	0.0
21	7.245589247545556E-4	0.0	0.0	0.004709633010904612	0.0
22	7.245589247545556E-4	0.0	0.0	0.006521030322791001	0.0
23	7.245589247545556E-4	0.0	0.0	0.008694707097054668	0.0
24	7.245589247545556E-4	0.0	0.0	0.012679781183204723	0.0
25	7.245589247545556E-4	0.0	0.0	0.014128899032713835	0.0
26	7.245589247545556E-4	0.0	0.0	0.015578016882222948	0.0
27	7.245589247545556E-4	0.0	0.0	0.030431474839691337	0.0
28	7.245589247545556E-4	0.0	0.0	0.10578560301416513	0.0
29	7.245589247545556E-4	0.0	0.0	0.21664311850161214	0.0
30	7.245589247545556E-4	0.0	0.0	0.3923486577545919	0.0
31	7.245589247545556E-4	0.0	0.0	0.9031626997065536	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATGG	20	0.0018395141	37.0	1
TTAGAAC	30	3.592457E-4	30.833334	3
TATGCGG	25	0.0054892	29.599998	5
CGTTAGA	25	0.0054892	29.599998	1
GGTATCA	360	0.0	28.777779	1
AACACCT	45	1.3196561E-4	24.666668	36
CGCGCAA	40	0.0019274914	23.125	21
CATACTG	40	0.0019274914	23.125	5
TACTCAC	40	0.0019274914	23.125	2
TGCGGGT	125	0.0	22.2	21
CTTATAC	275	0.0	21.527273	37
CACATAC	45	0.003818556	20.555557	3
TTTATAA	45	0.003818556	20.555557	2
CCGGTAT	45	0.003818556	20.555557	23
ATACGGC	45	0.003818556	20.555557	29
GCGGGTA	135	1.8189894E-12	20.555555	22
GTAACGT	135	1.8189894E-12	20.555555	26
TAATACG	210	0.0	20.261906	27
GGTAACG	140	3.6379788E-12	19.821428	25
GCGGTAA	225	0.0	19.733334	23
>>END_MODULE