FastQCFastQC Report
Fri 10 Feb 2017
ERR1631867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147716
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4760.32223997400417015No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4700.31817812559235287No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3570.24167998050312764No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3530.2389720815619161No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3440.23287930894419023No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3180.21527796582631537No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3060.20715426900268083No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2950.19970754691434914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.1922608248260175No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2830.1915838500907146No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2820.19090687535541173No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2770.18752200167889732No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2720.18413712800238294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2560.1733055322375369No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2400.16247393647269084No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2270.1536732649137534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2220.15028839123723903No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2170.14690351756072462No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2130.14419561861951313No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2120.14351864388421023No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2090.1414877196783016No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2060.13945679547239298No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2020.13674889653118144No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1950.13201007338406132No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1940.13133309864875842No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1900.1286251997075469No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1830.12388637656042677No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1800.12185545235451814No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1800.12185545235451814No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1790.12117847761921524No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1750.11847057867800373No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1700.11508570500148935No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1690.11440873026618648No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1640.11102385658967207No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1630.11034688185436921No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1610.10899293238376344No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1590.10763898291315768No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1590.10763898291315768No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1570.10628503344255194No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1540.1042541092366433No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1510.10222318503073466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2200.034.4772721
CTGTAGA405.905681E-527.751
AACGACC358.8326976E-426.4285737
TGCGGGT1051.8189894E-1224.66666621
TGTAGAA451.3156004E-424.6666662
TTCTGCG1051.8189894E-1224.66666618
CGGGTAA1103.6379788E-1223.54545423
GAACAGA551.8881457E-523.5454546
GCAGTCG400.001923272623.1259
AACAGAG652.6566559E-622.769237
GTAACGT1155.456968E-1222.5217426
AGAACAG502.6866843E-422.1999995
GTAGAAC502.6866843E-422.1999993
AGACTGA603.6980637E-521.5833326
GGTAACG1209.094947E-1221.58333225
CTGCGGG1209.094947E-1221.58333220
GCGGGTA1209.094947E-1221.58333222
ACGGGTC450.003810278620.55555517
CGCCTTA450.003810278620.55555525
GTATCAA3650.020.2739722
Produced by FastQC (version 0.11.5)