##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631867.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	147716
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919528013214546	31.0	31.0	34.0	30.0	34.0
2	32.101045248991305	33.0	31.0	34.0	30.0	34.0
3	32.18913320154892	34.0	31.0	34.0	30.0	34.0
4	35.85172222372661	37.0	35.0	37.0	35.0	37.0
5	35.56624874759674	37.0	35.0	37.0	33.0	37.0
6	35.59699017032684	37.0	35.0	37.0	33.0	37.0
7	35.614442579002954	37.0	35.0	37.0	33.0	37.0
8	35.55814536001517	37.0	35.0	37.0	33.0	37.0
9	37.290171680792874	39.0	37.0	39.0	34.0	39.0
10	37.13599068482764	39.0	37.0	39.0	34.0	39.0
11	37.22313764250318	39.0	37.0	39.0	34.0	39.0
12	37.13442010344174	39.0	37.0	39.0	34.0	39.0
13	37.22131658046521	39.0	37.0	39.0	34.0	39.0
14	38.37584960329281	40.0	38.0	41.0	34.0	41.0
15	38.343442822713854	40.0	38.0	41.0	34.0	41.0
16	38.267073302824336	40.0	38.0	41.0	34.0	41.0
17	38.23712394053454	40.0	38.0	41.0	34.0	41.0
18	38.28183812180129	40.0	38.0	41.0	34.0	41.0
19	38.309167591865474	40.0	38.0	41.0	34.0	41.0
20	38.28696959029489	40.0	38.0	41.0	34.0	41.0
21	38.22332042568171	40.0	38.0	41.0	34.0	41.0
22	38.196640851363426	40.0	38.0	41.0	34.0	41.0
23	38.12686506539576	40.0	38.0	41.0	34.0	41.0
24	38.114618592434134	40.0	38.0	41.0	34.0	41.0
25	38.06829998104471	40.0	38.0	41.0	34.0	41.0
26	37.961608762760974	40.0	37.0	41.0	33.0	41.0
27	37.81383871753906	40.0	37.0	41.0	33.0	41.0
28	37.830079341438974	40.0	37.0	41.0	33.0	41.0
29	37.78907498172168	40.0	37.0	41.0	33.0	41.0
30	37.7274161228303	39.0	37.0	41.0	33.0	41.0
31	37.6342237807685	39.0	37.0	40.0	33.0	41.0
32	37.60391562186899	39.0	37.0	40.0	33.0	41.0
33	37.489967234422814	39.0	37.0	40.0	33.0	41.0
34	37.47074792168756	39.0	37.0	40.0	32.0	41.0
35	37.36282460938558	39.0	36.0	40.0	32.0	41.0
36	37.32768962062336	39.0	36.0	40.0	32.0	41.0
37	37.278060602778304	39.0	36.0	40.0	32.0	41.0
38	37.23515394405481	39.0	36.0	40.0	32.0	41.0
39	37.15219069024344	39.0	36.0	40.0	32.0	41.0
40	36.81859108018089	39.0	35.0	40.0	31.0	41.0
41	36.900884129004304	39.0	35.0	40.0	31.0	41.0
42	36.863508353868234	39.0	35.0	40.0	31.0	41.0
43	36.297591323891794	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	2.0
18	5.0
19	9.0
20	20.0
21	43.0
22	66.0
23	105.0
24	181.0
25	304.0
26	428.0
27	674.0
28	921.0
29	1350.0
30	1871.0
31	2546.0
32	3554.0
33	4707.0
34	6733.0
35	10283.0
36	16325.0
37	28333.0
38	44518.0
39	24736.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.18974247881069	17.24863928078204	13.475182106203794	27.086436134203474
2	20.173170137290477	20.624035311002192	33.276693113812996	25.92610143789434
3	20.565815483766144	20.650437325679004	30.419859730834848	28.363887459720004
4	15.321292209374745	16.818760323864712	34.88383113542203	32.97611633133851
5	15.541308998348184	34.13848195185356	34.40250209862168	15.917706951176584
6	32.62476644371632	35.31641799128057	16.35706355438815	15.701752010614964
7	29.061171437081967	29.03138454872864	20.665330769855668	21.242113244333723
8	26.416231146253622	32.12515908906279	21.08640905521406	20.372200709469524
9	28.535839042486934	13.16309675322917	17.746215711229656	40.55484849305424
10	18.529475479975087	24.35078122884454	30.263478566979877	26.85626472420049
11	39.030978363887456	19.81437352757995	19.34184516233854	21.812802946194047
12	21.959029489019468	24.830079341438978	27.483820303826263	25.72707086571529
13	34.70714072950798	18.014297706409597	21.79452462834087	25.48403693574156
14	23.279130222860083	20.49608708602995	24.51731701372905	31.70746567738092
15	29.168133445259826	24.955996642205314	21.00652603644832	24.869343876086543
16	24.331148961520757	25.13201007338406	23.133580654769965	27.403260310325216
17	26.416908120988925	24.666928430230982	21.969184110049014	26.94697933873108
18	26.150857050014892	21.85139050610631	24.03801890113461	27.959733542744186
19	27.60567575618078	22.824203200736548	23.975737225486746	25.59438381759593
20	29.61832164423624	20.97335427441848	23.926995044544938	25.48132903680035
21	26.515069457607844	22.976522516179696	22.566275826586153	27.942132199626307
22	27.76137994530044	23.377291559478998	21.925857726989626	26.93547076823093
23	27.253648893823286	22.880392103766685	23.310271060684016	26.555687941726013
24	27.5867204635923	22.098486284491862	23.76993690595467	26.544856345961172
25	27.251617969617374	22.554767256086002	24.308131820520458	25.88548295377617
26	27.435755097619758	23.3305803027431	23.11665628638739	26.11700831324975
27	25.981613366189173	22.524303392997375	25.461019794741258	26.03306344607219
28	25.354057786563406	23.51607138021609	23.016464025562566	28.113406807657938
29	26.49069836713694	24.020417558016735	23.141027376858293	26.347856697988032
30	25.977551517777357	23.072652928592703	24.13956511143004	26.810230442199895
31	27.484497278561566	22.865498659590024	23.6358959083647	26.01410815348371
32	24.95058084432289	23.082130574886943	23.336673075360828	28.630615505429336
33	25.06431259985377	22.565598851850847	25.621462807008044	26.748625741287334
34	25.572043651330933	22.560183053968426	24.473313655934362	27.394459638766282
35	25.000676974735303	24.470605756993148	25.228817460532373	25.299899807739173
36	25.354734761298708	23.46191340139186	25.47320533997671	25.71014649733272
37	26.43721736304801	23.229711067182972	24.69942321752552	25.633648352243494
38	25.427171057976118	22.575753472880393	25.52600936933034	26.471066099813157
39	25.14351864388421	21.49327087113109	26.23006309404533	27.13314739093937
40	24.61750927455387	22.728072788323537	27.185274445557695	25.469143491564893
41	23.16065964418208	21.95158276693114	27.714668688564544	27.17308890032224
42	21.994909149990523	21.896747813371604	29.041539169758185	27.06680386687969
43	21.124319640391022	22.93251915838501	28.596766768664196	27.34639443255978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	4.0
4	6.0
5	6.0
6	12.5
7	19.0
8	20.5
9	22.0
10	35.0
11	48.0
12	48.0
13	93.5
14	139.0
15	307.0
16	475.0
17	463.5
18	452.0
19	452.0
20	485.5
21	519.0
22	449.5
23	380.0
24	414.0
25	448.0
26	448.0
27	511.0
28	574.0
29	829.0
30	1084.0
31	1250.0
32	1416.0
33	1416.0
34	1725.0
35	2034.0
36	2259.0
37	2484.0
38	3215.0
39	3946.0
40	3946.0
41	4735.0
42	5524.0
43	6272.5
44	7021.0
45	9880.5
46	12740.0
47	12740.0
48	13806.5
49	14873.0
50	16589.0
51	18305.0
52	18387.5
53	18470.0
54	18470.0
55	16059.5
56	13649.0
57	12688.5
58	11728.0
59	10578.5
60	9429.0
61	9429.0
62	8482.0
63	7535.0
64	6120.0
65	4705.0
66	4030.0
67	3355.0
68	3355.0
69	2822.0
70	2289.0
71	1941.5
72	1594.0
73	1255.0
74	916.0
75	916.0
76	738.5
77	561.0
78	468.0
79	375.0
80	313.5
81	252.0
82	252.0
83	214.0
84	176.0
85	134.0
86	92.0
87	71.5
88	51.0
89	51.0
90	36.0
91	21.0
92	13.0
93	5.0
94	3.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	147716.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.173657559099894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0756754004023	45.769584879092314
2	6.015583225116492	6.397411248612202
3	2.2483640159906297	3.5866121476346504
4	1.2374914063096785	2.6320777708575918
5	0.7689761413693886	2.0444637006146933
6	0.5983754742443917	1.9090687535541173
7	0.4099508568227535	1.5259010533726882
8	0.3386550556361877	1.4406022367245255
9	0.27881750821174855	1.3343172032819735
>10	1.7620248007536983	17.867394188848873
>50	0.16932752781809385	6.155731268109074
>100	0.09675858732462506	9.3368355492973
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	476	0.32223997400417015	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	470	0.31817812559235287	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	357	0.24167998050312764	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	353	0.2389720815619161	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	344	0.23287930894419023	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	318	0.21527796582631537	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	306	0.20715426900268083	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	295	0.19970754691434914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	284	0.1922608248260175	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	283	0.1915838500907146	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	282	0.19090687535541173	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	277	0.18752200167889732	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	272	0.18413712800238294	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	256	0.1733055322375369	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	240	0.16247393647269084	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	227	0.1536732649137534	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.15028839123723903	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	217	0.14690351756072462	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	213	0.14419561861951313	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	212	0.14351864388421023	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	209	0.1414877196783016	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	206	0.13945679547239298	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	202	0.13674889653118144	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	195	0.13201007338406132	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	194	0.13133309864875842	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	190	0.1286251997075469	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	183	0.12388637656042677	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	180	0.12185545235451814	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	180	0.12185545235451814	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	179	0.12117847761921524	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	175	0.11847057867800373	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	170	0.11508570500148935	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	169	0.11440873026618648	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	164	0.11102385658967207	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	163	0.11034688185436921	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	161	0.10899293238376344	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	159	0.10763898291315768	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	159	0.10763898291315768	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	157	0.10628503344255194	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	154	0.1042541092366433	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	151	0.10222318503073466	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.001353949470605757	0.0
16	0.0	0.0	0.0	0.002707898941211514	0.0
17	0.0	0.0	0.0	0.005415797882423028	0.0
18	0.0	0.0	0.0	0.005415797882423028	0.0
19	0.0	0.0	0.0	0.007446722088331663	0.0
20	0.0	0.0	0.0	0.008123696823634542	0.0
21	0.0	0.0	0.0	0.010154621029543178	0.0
22	0.0	0.0	0.0	0.016247393647269084	0.0
23	0.0	0.0	0.0	0.020309242059086356	0.0
24	0.0	0.0	0.0	0.02707898941211514	0.0
25	0.0	0.0	0.0	0.03046386308862953	0.0
26	0.0	0.0	0.0	0.0345257115004468	0.0
27	0.0	0.0	0.0	0.04671125673589862	0.0
28	0.0	0.0	0.0	0.11170083132497495	0.0
29	0.0	0.0	0.0	0.23220233420888733	0.0
30	0.0	0.0	0.0	0.37978282650491485	0.0
31	0.0	0.0	0.0	0.8401256465108722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	220	0.0	34.477272	1
CTGTAGA	40	5.905681E-5	27.75	1
AACGACC	35	8.8326976E-4	26.42857	37
TGCGGGT	105	1.8189894E-12	24.666666	21
TGTAGAA	45	1.3156004E-4	24.666666	2
TTCTGCG	105	1.8189894E-12	24.666666	18
CGGGTAA	110	3.6379788E-12	23.545454	23
GAACAGA	55	1.8881457E-5	23.545454	6
GCAGTCG	40	0.0019232726	23.125	9
AACAGAG	65	2.6566559E-6	22.76923	7
GTAACGT	115	5.456968E-12	22.52174	26
AGAACAG	50	2.6866843E-4	22.199999	5
GTAGAAC	50	2.6866843E-4	22.199999	3
AGACTGA	60	3.6980637E-5	21.583332	6
GGTAACG	120	9.094947E-12	21.583332	25
CTGCGGG	120	9.094947E-12	21.583332	20
GCGGGTA	120	9.094947E-12	21.583332	22
ACGGGTC	45	0.0038102786	20.555555	17
CGCCTTA	45	0.0038102786	20.555555	25
GTATCAA	365	0.0	20.273972	2
>>END_MODULE