FastQCFastQC Report
Fri 10 Feb 2017
ERR1631866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468228
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA23520.5023193828647582No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA23470.5012515270338382No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20530.4384616041757434No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19220.41048378140563996No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16550.3534602800345131No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15860.33872386956781736No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC15270.3261231707629616No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT15120.3229196032702017No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13800.29472820933391425No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA11040.23578256746713142No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10660.22766686315213955No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10370.22147329933280366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.21293045268544383No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9830.20994045635886793No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT9420.2011840385453241No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA9190.19627190172309214No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9120.19477690355980418No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG8940.19093262256849228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8920.19050548023612426No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG8830.18858333974046831No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG8750.18687477041099634No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC8730.18644762807862836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8630.1843119164167884No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT8610.18388477408442042No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC8520.18196263358876444No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA8430.1800404930931085No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG7870.16808050778680472No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC7620.16274122863220483No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7610.16252765746602083No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG7390.15782909180997293No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7120.15206267032300502No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6990.14928624516261307No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6970.1488591028302451No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6960.14864553166406108No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6500.1388212580195973No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA6480.1383941156872293No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6300.13454983469591736No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6220.13284126536644542No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT6210.1326276942002614No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5930.12664770154710953No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5910.12622055921474154No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG5830.12451198988526957No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5810.12408484755290158No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5700.12173556472487763No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT5690.12152199355869363No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5420.11575557207172574No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5390.11511485857317375No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5340.11404700274225378No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5270.11255200457896579No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5140.10977557941857385No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG5120.10934843708620588No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5000.10678558309199793No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4990.10657201192581392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCATG200.00184082237.07
CGAGCAA303.5962483E-430.83333410
ATGCCCC803.274181E-1127.75000237
GGTATCA11200.027.584821
ATTGCCG358.861514E-426.4285711
TACACCA451.3215766E-424.6666665
GAAATAC400.001929487123.12500217
TAGAACA802.7170245E-823.1250024
CCGTGTC400.001929487123.1250029
CCCTTTA400.001929487123.12500226
CTACCGT400.001929487123.1250028
GAGCAAT400.001929487123.12500211
TTTTGAC400.001929487123.1250022
GCGGTAA4350.021.68965523
TAAACCA603.720216E-521.5833345
CGCGGTA4400.021.44318222
ATACGGC957.1177055E-921.42105329
ATACACA957.1177055E-921.42105337
TATACAC2350.021.25531837
CCGCGGT4550.021.14285721
Produced by FastQC (version 0.11.5)