##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631866.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468228
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.606967118583256	34.0	31.0	34.0	31.0	34.0
2	32.779442920970126	34.0	31.0	34.0	31.0	34.0
3	32.855459306149996	34.0	31.0	34.0	31.0	34.0
4	36.2799704417506	37.0	37.0	37.0	35.0	37.0
5	36.20553234748883	37.0	35.0	37.0	35.0	37.0
6	36.25657372049514	37.0	37.0	37.0	35.0	37.0
7	36.25832286834619	37.0	37.0	37.0	35.0	37.0
8	36.22872404042475	37.0	37.0	37.0	35.0	37.0
9	38.0732442314428	39.0	38.0	39.0	37.0	39.0
10	38.011197536243024	39.0	38.0	39.0	35.0	39.0
11	38.08928342602322	39.0	38.0	39.0	37.0	39.0
12	38.01508026004425	39.0	38.0	39.0	35.0	39.0
13	38.080454394013174	39.0	38.0	39.0	37.0	39.0
14	39.4250151635528	40.0	39.0	41.0	37.0	41.0
15	39.447262871934186	40.0	39.0	41.0	37.0	41.0
16	39.37764721460485	40.0	39.0	41.0	36.0	41.0
17	39.34992567723417	40.0	39.0	41.0	36.0	41.0
18	39.39907694541975	40.0	39.0	41.0	37.0	41.0
19	39.4051017880178	40.0	39.0	41.0	37.0	41.0
20	39.43760304808768	40.0	39.0	41.0	37.0	41.0
21	39.37900766293344	40.0	39.0	41.0	37.0	41.0
22	39.36516825136472	40.0	39.0	41.0	36.0	41.0
23	39.30531279632999	40.0	39.0	41.0	36.0	41.0
24	39.30294642780868	40.0	39.0	41.0	36.0	41.0
25	39.27043448918049	40.0	39.0	41.0	36.0	41.0
26	39.20924635006877	40.0	39.0	41.0	36.0	41.0
27	39.103430380071245	40.0	39.0	41.0	36.0	41.0
28	39.10318263751847	40.0	39.0	41.0	35.0	41.0
29	39.10049164082456	40.0	39.0	41.0	35.0	41.0
30	39.054430747413655	40.0	39.0	41.0	35.0	41.0
31	38.96052777706588	40.0	38.0	41.0	35.0	41.0
32	38.92435309293763	40.0	38.0	41.0	35.0	41.0
33	38.84708304501226	40.0	38.0	41.0	35.0	41.0
34	38.84150883757486	40.0	38.0	41.0	35.0	41.0
35	38.77241642960267	40.0	38.0	41.0	35.0	41.0
36	38.733021092288375	40.0	38.0	41.0	35.0	41.0
37	38.69031967332155	40.0	38.0	41.0	35.0	41.0
38	38.66058415985375	40.0	38.0	41.0	35.0	41.0
39	38.596380395875514	40.0	38.0	41.0	35.0	41.0
40	38.27889404307303	40.0	38.0	41.0	34.0	41.0
41	38.364894880272004	40.0	38.0	41.0	34.0	41.0
42	38.357061089896376	40.0	38.0	41.0	34.0	41.0
43	37.91106469497766	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	0.0
15	1.0
16	1.0
17	0.0
18	4.0
19	8.0
20	12.0
21	19.0
22	67.0
23	102.0
24	190.0
25	305.0
26	493.0
27	878.0
28	1330.0
29	2036.0
30	3060.0
31	4259.0
32	5771.0
33	7778.0
34	11640.0
35	17621.0
36	26690.0
37	49215.0
38	110327.0
39	226418.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.76432848953929	16.33221422042253	14.342158093920057	28.561299196118128
2	19.85613846245846	19.768574284323023	33.01831586321194	27.356971390006578
3	19.8862519968904	19.738033607558712	31.135686033300015	29.240028362250868
4	14.560641396926282	17.43530929376287	35.75352178853037	32.25052752078047
5	15.847621244351043	32.689416267288586	35.170045362515694	16.292917125844674
6	30.74186080285673	35.30053734505412	17.43637714959379	16.521224702495367
7	28.577530604748112	28.431447927078263	21.56812493058937	21.422896537584254
8	26.79912350393398	30.345045576086864	22.117216398848424	20.73861452113073
9	29.630222882869028	12.250228521147816	18.397660968587953	39.721887627395205
10	19.039228751804675	23.640619527238865	28.929282315453154	28.3908694055033
11	39.014112782661435	19.36940977472513	18.353451737187864	23.263025705425562
12	21.725954022399343	25.16893479245154	26.642575839121115	26.462535346028005
13	35.991653638825525	17.75353033137702	20.92079072588568	25.334025303911766
14	23.099003049796252	22.50826520413132	24.51263059876812	29.880101147304305
15	30.574421008568475	24.58353622594121	20.292464354972363	24.549578410517952
16	23.097294480466783	25.55272217808418	23.604739571319953	27.74524377012908
17	26.49243530929376	24.903465832884834	21.54569995814005	27.05839889968135
18	26.893735530553492	20.78495946419266	23.351871310558103	28.969433694695745
19	27.17372732942071	22.620176495211737	24.00262265392074	26.203473521446817
20	31.44856779175957	19.72265648359346	23.296769949682634	25.532005774964333
21	26.771999965828613	22.50442092314001	22.03413721520285	28.689441895828526
22	29.22144767079286	23.02724313795843	21.407092271286636	26.344216919962072
23	27.919090699402854	22.036700069197057	22.73913563477622	27.30507359662387
24	26.882202687579554	21.511101429218243	24.87442015428381	26.732275728918392
25	26.536858111860035	21.939738759749524	24.625396174513273	26.898006953877168
26	27.008850389126664	23.828775724646967	22.85510477801413	26.30726910821224
27	25.285758220354186	21.79087965691928	26.60456017154036	26.31880195118617
28	24.882535858598803	23.008662446500423	22.465550970894522	29.643250724006254
29	26.824324901543694	23.841376423451823	23.192120078252476	26.142178596752007
30	25.89101890531963	21.759057553157863	24.29329301109716	28.05663053042535
31	27.957960651648346	22.881587602620947	23.43879477519499	25.721656970535722
32	25.22360901099464	22.706459246350068	22.70005211136455	29.36987963129074
33	24.588875505095807	21.610625592659986	26.881775545247187	26.91872335699702
34	25.44444159682889	21.75777612616076	25.09290345729004	27.704878819720307
35	23.995147663104298	24.77767241600246	26.573165210111316	24.654014710781926
36	24.89406870157274	23.20899220038101	26.306628394713687	25.590310703332563
37	25.45661515330138	23.75722938397533	25.595222840154797	25.19093262256849
38	25.19712618638783	22.25390194520618	25.971321663804815	26.577650204601177
39	24.863741595974613	20.362942839813083	27.301229315632554	27.472086248579753
40	24.09509896887841	22.32822471103821	28.037409125468788	25.539267194614588
41	21.880365975550372	20.759117353084395	29.44740596461553	27.913110706749706
42	20.10302673056716	21.04231271944437	31.130560325311603	27.724100224676867
43	19.06464372058057	22.45060098926164	30.743782943352382	27.740972346805403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	8.5
2	8.0
3	26.0
4	44.0
5	44.0
6	48.5
7	53.0
8	65.0
9	77.0
10	120.5
11	164.0
12	164.0
13	345.5
14	527.0
15	1094.5
16	1662.0
17	1645.0
18	1628.0
19	1628.0
20	1773.5
21	1919.0
22	1460.0
23	1001.0
24	1021.0
25	1041.0
26	1041.0
27	1202.5
28	1364.0
29	1689.5
30	2015.0
31	2285.5
32	2556.0
33	2556.0
34	3280.5
35	4005.0
36	4439.5
37	4874.0
38	6458.0
39	8042.0
40	8042.0
41	9931.5
42	11821.0
43	14868.0
44	17915.0
45	31347.5
46	44780.0
47	44780.0
48	50495.5
49	56211.0
50	62589.0
51	68967.0
52	67513.5
53	66060.0
54	66060.0
55	55959.5
56	45859.0
57	42240.5
58	38622.0
59	34332.0
60	30042.0
61	30042.0
62	25724.0
63	21406.0
64	16973.0
65	12540.0
66	10532.5
67	8525.0
68	8525.0
69	7005.0
70	5485.0
71	4631.5
72	3778.0
73	2704.5
74	1631.0
75	1631.0
76	1369.0
77	1107.0
78	972.5
79	838.0
80	755.0
81	672.0
82	672.0
83	565.0
84	458.0
85	351.0
86	244.0
87	209.5
88	175.0
89	175.0
90	125.0
91	75.0
92	48.5
93	22.0
94	14.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	468228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.262110164300644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1823218611191	29.438422987216136
2	7.942558220844822	5.760278423812934
3	2.913716835567191	3.1697256263674487
4	1.6827142661867305	2.4407508038201415
5	1.0855762422335489	1.9682642643810235
6	0.7294693914773785	1.587125966114283
7	0.5431460314185433	1.3786934858621342
8	0.43550057483899685	1.263373585714237
9	0.3692180581071747	1.204976330795837
>10	2.5596945468687693	18.37314380328041
>50	0.30079356154187875	7.659187071943488
>100	0.22515195863940232	15.511021706353462
>500	0.02304705088434827	6.057576524808306
>1k	0.00709140027210716	4.18745941953018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2352	0.5023193828647582	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2347	0.5012515270338382	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2053	0.4384616041757434	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1922	0.41048378140563996	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1655	0.3534602800345131	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1586	0.33872386956781736	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1527	0.3261231707629616	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1512	0.3229196032702017	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1380	0.29472820933391425	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1104	0.23578256746713142	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1066	0.22766686315213955	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1037	0.22147329933280366	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.21293045268544383	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	983	0.20994045635886793	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	942	0.2011840385453241	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	919	0.19627190172309214	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	912	0.19477690355980418	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	894	0.19093262256849228	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	892	0.19050548023612426	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	883	0.18858333974046831	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	875	0.18687477041099634	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	873	0.18644762807862836	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	863	0.1843119164167884	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	861	0.18388477408442042	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	852	0.18196263358876444	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	843	0.1800404930931085	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	787	0.16808050778680472	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	762	0.16274122863220483	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	761	0.16252765746602083	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG	739	0.15782909180997293	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	712	0.15206267032300502	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	699	0.14928624516261307	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	697	0.1488591028302451	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	696	0.14864553166406108	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	650	0.1388212580195973	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	648	0.1383941156872293	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	630	0.13454983469591736	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	622	0.13284126536644542	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	621	0.1326276942002614	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	593	0.12664770154710953	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	591	0.12622055921474154	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	583	0.12451198988526957	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	581	0.12408484755290158	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	570	0.12173556472487763	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	569	0.12152199355869363	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	542	0.11575557207172574	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	539	0.11511485857317375	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	534	0.11404700274225378	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	527	0.11255200457896579	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	514	0.10977557941857385	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	512	0.10934843708620588	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	500	0.10678558309199793	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	499	0.10657201192581392	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.271423323679917E-4	0.0	0.0	0.0	0.0
9	4.271423323679917E-4	0.0	0.0	0.0	0.0
10	6.407134985519875E-4	0.0	0.0	0.0	0.0
11	8.542846647359833E-4	0.0	0.0	0.0	0.0
12	0.0010678558309199792	0.0	0.0	0.0	0.0
13	0.0010678558309199792	0.0	0.0	0.0	0.0
14	0.0010678558309199792	0.0	0.0	0.0	0.0
15	0.001281426997103975	0.0	0.0	2.1357116618399584E-4	0.0
16	0.001281426997103975	0.0	0.0	0.0017085693294719667	0.0
17	0.001281426997103975	0.0	0.0	0.0032035674927599375	0.0
18	0.0014949981632879708	0.0	0.0	0.004057852157495921	0.0
19	0.0014949981632879708	0.0	0.0	0.006407134985519875	0.0
20	0.0014949981632879708	0.0	0.0	0.009610702478279812	0.0
21	0.002349282828023954	0.0	0.0	0.018153549125639646	0.0
22	0.002349282828023954	0.0	0.0	0.0256285399420795	0.0
23	0.002349282828023954	0.0	0.0	0.028832107434839438	0.0
24	0.002349282828023954	0.0	0.0	0.035025671254175314	2.1357116618399584E-4
25	0.002349282828023954	0.0	0.0	0.03993780807640722	2.1357116618399584E-4
26	0.002349282828023954	0.0	0.0	0.04570422956337511	2.1357116618399584E-4
27	0.002349282828023954	0.0	0.0	0.06770205968032668	2.1357116618399584E-4
28	0.002349282828023954	0.0	0.0	0.17427407160614058	2.1357116618399584E-4
29	0.00256285399420795	0.0	0.0	0.3784481064780406	2.1357116618399584E-4
30	0.00256285399420795	0.0	0.0	0.6370827887268595	2.1357116618399584E-4
31	0.00256285399420795	0.0	0.0	1.3365283579794458	2.1357116618399584E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCATG	20	0.001840822	37.0	7
CGAGCAA	30	3.5962483E-4	30.833334	10
ATGCCCC	80	3.274181E-11	27.750002	37
GGTATCA	1120	0.0	27.58482	1
ATTGCCG	35	8.861514E-4	26.42857	11
TACACCA	45	1.3215766E-4	24.666666	5
GAAATAC	40	0.0019294871	23.125002	17
TAGAACA	80	2.7170245E-8	23.125002	4
CCGTGTC	40	0.0019294871	23.125002	9
CCCTTTA	40	0.0019294871	23.125002	26
CTACCGT	40	0.0019294871	23.125002	8
GAGCAAT	40	0.0019294871	23.125002	11
TTTTGAC	40	0.0019294871	23.125002	2
GCGGTAA	435	0.0	21.689655	23
TAAACCA	60	3.720216E-5	21.583334	5
CGCGGTA	440	0.0	21.443182	22
ATACGGC	95	7.1177055E-9	21.421053	29
ATACACA	95	7.1177055E-9	21.421053	37
TATACAC	235	0.0	21.255318	37
CCGCGGT	455	0.0	21.142857	21
>>END_MODULE