FastQCFastQC Report
Fri 10 Feb 2017
ERR1631865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300298
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6310.2101246095545092No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4970.16550226774737095No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT4910.16350425244257372No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4880.16250524479017509No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4520.15051715296139168No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4140.1378630560310092No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3750.12487595654982718No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3620.12054692338943317No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3330.11088984941624654No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG3280.10922483666224883No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3180.10589481115425345No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3100.10323079074785714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG250.005489958529.625
GGTATCA3400.028.8382361
ATCAGAA2150.027.53488317
ACAGGCT2300.026.5434788
TGCGGGT856.91216E-1126.11764721
CATCAGA2300.025.7391316
GGACAGG2550.025.3921576
TTCTGCG901.4188117E-1024.66666818
GACAGGC2550.023.9411757
AGAAGAG2400.023.89583220
TGCATCA2550.023.21568714
TCAGAAG2550.023.21568718
ACCCATT400.001927883423.12500219
ATACACA652.671899E-622.7692337
TTCTCGT652.671899E-622.7692311
ATCAAGC2450.022.6530630
GATGCTA903.799869E-922.61111314
CTGCGGG1005.293259E-1022.220
AGGCCAT2500.022.19999925
TATACAC1250.022.19999937
Produced by FastQC (version 0.11.5)