##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631865.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	300298
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.444834797434545	33.0	31.0	34.0	31.0	34.0
2	32.625348820171965	34.0	31.0	34.0	31.0	34.0
3	32.70673131356186	34.0	31.0	34.0	31.0	34.0
4	36.191836109464596	37.0	35.0	37.0	35.0	37.0
5	36.090606664047044	37.0	35.0	37.0	35.0	37.0
6	36.139444818147304	37.0	35.0	37.0	35.0	37.0
7	36.130716821290854	37.0	35.0	37.0	35.0	37.0
8	36.10894178449407	37.0	35.0	37.0	35.0	37.0
9	37.93884408154567	39.0	38.0	39.0	35.0	39.0
10	37.83943283005548	39.0	38.0	39.0	35.0	39.0
11	37.92484798433556	39.0	38.0	39.0	35.0	39.0
12	37.86107799585745	39.0	38.0	39.0	35.0	39.0
13	37.89690574029797	39.0	38.0	39.0	35.0	39.0
14	39.263774650513824	40.0	39.0	41.0	36.0	41.0
15	39.255432936616295	40.0	39.0	41.0	36.0	41.0
16	39.20616853925101	40.0	39.0	41.0	36.0	41.0
17	39.15367401714297	40.0	39.0	41.0	36.0	41.0
18	39.1572671146661	40.0	39.0	41.0	36.0	41.0
19	39.17480968904222	40.0	39.0	41.0	36.0	41.0
20	39.15870901571106	40.0	39.0	41.0	36.0	41.0
21	39.132225322845976	40.0	39.0	41.0	36.0	41.0
22	39.09369692771847	40.0	39.0	41.0	36.0	41.0
23	39.04332363185902	40.0	39.0	41.0	36.0	41.0
24	39.05322046766878	40.0	39.0	41.0	36.0	41.0
25	38.98689967965154	40.0	38.0	41.0	35.0	41.0
26	38.91654290071862	40.0	38.0	41.0	35.0	41.0
27	38.82663554202825	40.0	38.0	41.0	35.0	41.0
28	38.79354840858081	40.0	38.0	41.0	35.0	41.0
29	38.7717633817075	40.0	38.0	41.0	35.0	41.0
30	38.716691419856275	40.0	38.0	41.0	35.0	41.0
31	38.66034405823549	40.0	38.0	41.0	35.0	41.0
32	38.62771979833366	40.0	38.0	41.0	35.0	41.0
33	38.56073300521482	40.0	38.0	41.0	35.0	41.0
34	38.527805712991764	40.0	38.0	41.0	34.0	41.0
35	38.431518025428076	40.0	38.0	41.0	34.0	41.0
36	38.41543067219895	40.0	38.0	41.0	34.0	41.0
37	38.37822762722362	40.0	38.0	41.0	34.0	41.0
38	38.337045201766244	40.0	38.0	41.0	34.0	41.0
39	38.293658299422574	40.0	38.0	41.0	34.0	41.0
40	37.951354987379204	40.0	37.0	41.0	33.0	41.0
41	38.04621409399996	40.0	37.0	41.0	33.0	41.0
42	38.04097263385037	40.0	37.0	41.0	33.0	41.0
43	37.552837514735366	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	4.0
19	3.0
20	7.0
21	16.0
22	48.0
23	97.0
24	171.0
25	289.0
26	486.0
27	777.0
28	1128.0
29	1705.0
30	2328.0
31	3224.0
32	4352.0
33	6004.0
34	8935.0
35	13481.0
36	19960.0
37	34624.0
38	78438.0
39	124218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.175445723914244	16.265509593803486	12.354727637213701	28.204317045068567
2	20.709428634223336	18.219568561895183	33.07381334541022	27.99718945847125
3	20.899240088179074	19.874924241919693	28.72713104982384	30.498704620077387
4	15.804301060946127	14.96346962017729	33.35120447022624	35.88102484865034
5	17.06005368001119	32.850368633823734	33.39049877122059	16.699078914944486
6	36.27463386369539	33.00687983269952	14.505924115378724	16.21256218822636
7	31.72515301467209	27.24660170896909	19.694769861937143	21.333475414421674
8	28.644879419776352	30.87399849482847	19.178282905647055	21.30283917974812
9	28.804054639058535	12.56485224676821	17.01576434075485	41.615328773418405
10	19.52327354827538	23.28653537486097	29.79040819452677	27.399782882336876
11	39.7365283818074	19.099028298556767	18.427695156144896	22.736748163490933
12	24.716781330544993	23.08340381887325	25.459044016277165	26.740770834304588
13	33.44078215639132	17.336778799725604	21.159981085455115	28.06245795842796
14	24.257903815543227	18.888903689002255	22.94887078835024	33.90432170710428
15	29.789742189425173	23.238583007545838	20.18128658865527	26.790388214373724
16	26.82268946180128	23.32349865799972	21.06274434062165	28.79106753957735
17	26.953559464265496	23.640517086360884	21.535607962756995	27.870315486616626
18	26.751426915930175	21.312496253721303	23.349472856962084	28.58660397338644
19	28.475714123970192	21.551592085195374	22.65116650793545	27.321527282898987
20	28.80105761610134	20.781690187746836	23.055431604606092	27.361820591545733
21	28.64454641722556	21.73241247027952	21.541935011222186	28.081106101272734
22	29.373822003476548	22.72342806145895	20.623180973566257	27.279568961498246
23	28.433089797467847	22.36345230404465	21.548928064788978	27.654529833698525
24	28.850341993619672	21.710101299375953	21.317491291983295	28.12206541502108
25	28.099088239015913	21.334141419523274	22.74540622981172	27.821364111649093
26	28.577612904514847	21.995817487961958	22.53061958454602	26.89595002297718
27	28.658199521808335	21.317824294534095	22.55925780391478	27.464718379742788
28	26.67750034965268	21.79268593197424	22.68146974005821	28.848343978314873
29	28.33285602967719	22.209938128126062	22.68080373495661	26.776402107240145
30	27.033480076457383	21.22824660836902	23.818007445937038	27.92026586923656
31	27.153027992194424	22.18829296232409	22.93355267101346	27.72512637446803
32	25.60723015138296	21.989157436945966	23.073413742349267	29.330198669321806
33	26.617892893059558	21.91589687577007	24.097396586057847	27.36881364511252
34	26.93790834437792	21.53760597806179	24.10339063197224	27.42109504558805
35	26.798047273042116	22.79469060733005	24.2039574023137	26.203304717314136
36	26.584259635428808	21.79268593197424	24.04678019833632	27.576274234260634
37	25.5056643733891	21.68379409786279	24.731433442780173	28.079108085967942
38	25.87629621242899	20.640830108758635	25.761410332403145	27.72146334640923
39	24.73809349379616	20.321813665092677	26.78572617866253	28.15436666244863
40	24.683814078015835	20.83830062138276	27.92392889729535	26.55395640330605
41	23.174313515241526	19.561568841617326	28.94691273335154	28.317204909789613
42	21.606204503526495	20.698106547496153	29.615248852806214	28.080440096171138
43	21.420056077629553	19.83962597153494	29.880651885793448	28.859666065042056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	4.0
2	2.0
3	9.5
4	17.0
5	17.0
6	18.0
7	19.0
8	18.0
9	17.0
10	32.5
11	48.0
12	48.0
13	93.0
14	138.0
15	269.5
16	401.0
17	407.5
18	414.0
19	414.0
20	443.0
21	472.0
22	403.0
23	334.0
24	373.0
25	412.0
26	412.0
27	518.0
28	624.0
29	831.5
30	1039.0
31	1557.5
32	2076.0
33	2076.0
34	2931.0
35	3786.0
36	4588.0
37	5390.0
38	6880.0
39	8370.0
40	8370.0
41	9425.0
42	10480.0
43	12607.0
44	14734.0
45	17368.5
46	20003.0
47	20003.0
48	21826.0
49	23649.0
50	27085.0
51	30521.0
52	31475.0
53	32429.0
54	32429.0
55	30888.5
56	29348.0
57	27595.5
58	25843.0
59	23808.5
60	21774.0
61	21774.0
62	20646.0
63	19518.0
64	16174.0
65	12830.0
66	11246.5
67	9663.0
68	9663.0
69	8254.5
70	6846.0
71	6364.0
72	5882.0
73	6091.0
74	6300.0
75	6300.0
76	5290.5
77	4281.0
78	2872.0
79	1463.0
80	990.0
81	517.0
82	517.0
83	421.0
84	325.0
85	247.5
86	170.0
87	129.0
88	88.0
89	88.0
90	68.0
91	48.0
92	33.5
93	19.0
94	10.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	300298.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.03089845380704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.02700363087654	43.900352865725495
2	6.151046368340342	6.277868452148691
3	2.2538200726948925	3.4504338978853513
4	1.1770204574825942	2.402576457753911
5	0.7897366156448924	2.015048451911387
6	0.5106542296399941	1.5635486482659358
7	0.37773944037835283	1.3493468116761935
8	0.3220060500909511	1.3145806434962262
9	0.24435472178685047	1.1222676894791732
>10	1.8039453067153737	18.17954809611138
>50	0.21563649848211808	7.649362387575495
>100	0.12638513808317495	10.56528909023205
>500	6.514697839338915E-4	0.20977650773871692
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	631	0.2101246095545092	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	497	0.16550226774737095	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	491	0.16350425244257372	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	488	0.16250524479017509	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	452	0.15051715296139168	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	414	0.1378630560310092	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	375	0.12487595654982718	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	362	0.12054692338943317	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	333	0.11088984941624654	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	328	0.10922483666224883	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	318	0.10589481115425345	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	310	0.10323079074785714	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.330025507995391E-4	0.0
16	0.0	0.0	0.0	3.330025507995391E-4	0.0
17	0.0	0.0	0.0	0.0013320102031981564	0.0
18	0.0	0.0	0.0	0.001998015304797235	0.0
19	0.0	0.0	0.0	0.002331017855596774	0.0
20	0.0	0.0	0.0	0.002664020406396313	0.0
21	0.0	0.0	0.0	0.005994045914391705	0.0
22	3.330025507995391E-4	0.0	0.0	0.01198809182878341	0.0
23	3.330025507995391E-4	0.0	0.0	0.014319109684380183	0.0
24	3.330025507995391E-4	0.0	0.0	0.016650127539976956	0.0
25	3.330025507995391E-4	0.0	0.0	0.01864814284477419	0.0
26	3.330025507995391E-4	0.0	0.0	0.024309186208366355	0.0
27	3.330025507995391E-4	0.0	0.0	0.052281400475527644	0.0
28	3.330025507995391E-4	0.0	0.0	0.17549234427135713	0.0
29	3.330025507995391E-4	0.0	0.0	0.36297278037149766	0.0
30	3.330025507995391E-4	0.0	0.0	0.5797574409419977	0.0
31	6.660051015990782E-4	0.0	0.0	1.1881531012527555	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAACG	25	0.0054899585	29.6	25
GGTATCA	340	0.0	28.838236	1
ATCAGAA	215	0.0	27.534883	17
ACAGGCT	230	0.0	26.543478	8
TGCGGGT	85	6.91216E-11	26.117647	21
CATCAGA	230	0.0	25.73913	16
GGACAGG	255	0.0	25.392157	6
TTCTGCG	90	1.4188117E-10	24.666668	18
GACAGGC	255	0.0	23.941175	7
AGAAGAG	240	0.0	23.895832	20
TGCATCA	255	0.0	23.215687	14
TCAGAAG	255	0.0	23.215687	18
ACCCATT	40	0.0019278834	23.125002	19
ATACACA	65	2.671899E-6	22.76923	37
TTCTCGT	65	2.671899E-6	22.76923	11
ATCAAGC	245	0.0	22.65306	30
GATGCTA	90	3.799869E-9	22.611113	14
CTGCGGG	100	5.293259E-10	22.2	20
AGGCCAT	250	0.0	22.199999	25
TATACAC	125	0.0	22.199999	37
>>END_MODULE