FastQCFastQC Report
Fri 10 Feb 2017
ERR1631864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131628
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4220.32060048014100345No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4190.31832132980824746No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4150.3152824626979062No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3770.2864132251496642No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3560.2704591728203726No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3400.2583037043790075No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3180.24158993527213052No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3120.23703163460661864No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2980.22639559972042422No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2890.21955814872215637No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2740.20816239705837664No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2600.19752636217218222No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2380.18081259306530525No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2230.1694168414015255No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2120.16105995684808702No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2040.1549822226274045No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1940.14738505485155134No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1910.14510590451879538No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1890.14358647096362476No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1880.14282675418603943No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1850.1405476038532835No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1800.1367490199653569No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1770.13446986963260096No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1770.13446986963260096No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1740.13219071929984502No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1740.13219071929984502No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1610.12231440119123592No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1540.1169963837481387No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1520.11547695019296807No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1510.11471723341538276No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1490.11319779986021211No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1490.11319779986021211No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1480.11243808308262679No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1470.11167836630504148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.11015893274987085No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1430.10863949919470023No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1420.10787978241711489No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1410.10712006563952958No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1410.10712006563952958No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1390.10560063208435895No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1350.1025617649740177No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1330.10104233141884705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1330.10104233141884705No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1330.10104233141884705No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1330.10104233141884705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT507.228664E-933.30000330
ATACGGC451.0676558E-732.8888929
AGTCGGT303.582335E-430.83333411
GGTATCA2050.030.6829281
CTATGCC250.00547889429.64
GGCCGTT250.00547889429.614
TTCGTGT250.00547889429.616
AAGTTCG250.00547889429.628
ACGAAAG250.00547889429.619
CTCTATG652.9394869E-928.4615381
CAGTCGG358.827555E-426.4285710
TCTATGG706.4792403E-926.428572
AGCCGCG2750.024.21818419
GCGGTAA2750.024.21818423
AACTCCG2750.024.2181845
CGTGCCA2800.023.78571310
GCCAGCA2800.023.78571313
GTGCCAG2800.023.78571311
ACGGTGT551.8861148E-523.5454548
GGTAATA2850.023.36842225
Produced by FastQC (version 0.11.5)