##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631864.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131628
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46668641930288	33.0	31.0	34.0	31.0	34.0
2	32.64098823958428	34.0	31.0	34.0	31.0	34.0
3	32.71618500622968	34.0	31.0	34.0	31.0	34.0
4	36.18832619199562	37.0	35.0	37.0	35.0	37.0
5	36.09077855775367	37.0	35.0	37.0	35.0	37.0
6	36.156721974048075	37.0	35.0	37.0	35.0	37.0
7	36.15374388427994	37.0	36.0	37.0	35.0	37.0
8	36.134279940438205	37.0	35.0	37.0	35.0	37.0
9	37.9538775944328	39.0	38.0	39.0	35.0	39.0
10	37.87295256328441	39.0	38.0	39.0	35.0	39.0
11	37.959704622116874	39.0	38.0	39.0	35.0	39.0
12	37.87208648615796	39.0	38.0	39.0	35.0	39.0
13	37.94267177196341	39.0	38.0	39.0	35.0	39.0
14	39.28301729115386	40.0	39.0	41.0	36.0	41.0
15	39.298287598383325	40.0	39.0	41.0	36.0	41.0
16	39.235724921749174	40.0	39.0	41.0	36.0	41.0
17	39.215432886619865	40.0	39.0	41.0	36.0	41.0
18	39.240100890388064	40.0	39.0	41.0	36.0	41.0
19	39.243869085604885	40.0	39.0	41.0	36.0	41.0
20	39.24927826906129	40.0	39.0	41.0	36.0	41.0
21	39.21111009815541	40.0	39.0	41.0	36.0	41.0
22	39.18424651289999	40.0	39.0	41.0	36.0	41.0
23	39.12147871273589	40.0	39.0	41.0	36.0	41.0
24	39.13250980034643	40.0	39.0	41.0	36.0	41.0
25	39.0816467620871	40.0	39.0	41.0	36.0	41.0
26	39.01171483271037	40.0	39.0	41.0	36.0	41.0
27	38.87252712188896	40.0	38.0	41.0	35.0	41.0
28	38.884241954599325	40.0	38.0	41.0	35.0	41.0
29	38.86259762360592	40.0	38.0	41.0	35.0	41.0
30	38.82081320083873	40.0	38.0	41.0	35.0	41.0
31	38.738034460753035	40.0	38.0	41.0	35.0	41.0
32	38.70193271948218	40.0	38.0	41.0	35.0	41.0
33	38.64001580210898	40.0	38.0	41.0	35.0	41.0
34	38.62214726350017	40.0	38.0	41.0	35.0	41.0
35	38.545932476372805	40.0	38.0	41.0	35.0	41.0
36	38.501633391071806	40.0	38.0	41.0	34.0	41.0
37	38.45833713191722	40.0	38.0	41.0	34.0	41.0
38	38.41480536056158	40.0	38.0	41.0	34.0	41.0
39	38.31886072872033	40.0	38.0	41.0	34.0	41.0
40	38.004398760142216	40.0	37.0	41.0	33.0	41.0
41	38.100039505272434	40.0	37.0	41.0	34.0	41.0
42	38.09770717476525	40.0	37.0	41.0	34.0	41.0
43	37.60134621812988	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	13.0
22	22.0
23	46.0
24	68.0
25	131.0
26	198.0
27	293.0
28	493.0
29	725.0
30	940.0
31	1316.0
32	1812.0
33	2577.0
34	3604.0
35	5475.0
36	8365.0
37	15151.0
38	34781.0
39	55610.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.33369799738658	16.414440696508343	13.185644391770749	28.066216914334337
2	21.08745859543562	19.94864314583523	31.978758318898713	26.985139939830432
3	21.223447898623395	19.813413559425047	29.86598596043395	29.09715258151761
4	15.410095116540553	16.562585468137478	35.25921536451211	32.76810405080986
5	16.126508037803507	33.2885100434558	34.179657823563375	16.405324095177317
6	32.162609779074366	34.87631810860911	16.258698757103353	16.702373355213176
7	28.956604977664323	28.140669158537694	20.559455435013827	22.343270428784148
8	26.884097608411583	30.423618075181576	21.31005561126812	21.382228705138726
9	28.493177743337284	12.49582155772328	17.674051113744795	41.33694958519464
10	19.368979244537638	23.19795180356763	29.317470447017353	28.115598504877383
11	39.24772844683502	19.50952684839092	18.673078676269487	22.569666028504574
12	22.14194548272404	24.320813200838728	26.77621782599447	26.761023490442764
13	35.66566384052025	17.514510590451877	20.559455435013827	26.26037013401404
14	23.88701492083751	20.61567447655514	23.474488710608686	32.02282189199867
15	29.92448415230802	24.128604856109643	20.021575956483424	25.925335035098918
16	24.68319810374692	24.734858844622725	22.89482480931109	27.687118242319265
17	26.9403166499529	24.17570729631993	21.680797398729755	27.203178654997416
18	26.494362901510314	21.041875588780503	23.401555899960496	29.062205609748688
19	27.926429027258635	22.470902847418483	23.755583918315253	25.847084207007626
20	30.69939526544504	20.27987966086243	22.76643267389917	26.254292399793357
21	27.161394232230222	22.303765156349716	21.76436624426414	28.770474367155924
22	28.529644148661383	23.080195703041905	21.00844805056675	27.381712097729967
23	28.04342541100678	22.26046130002735	22.185249339046404	27.510863949919468
24	27.701552861093386	21.302458443492267	23.223022457227945	27.772966238186402
25	28.063178047223996	21.882122344789863	23.43042513750874	26.624274470477406
26	27.972771750691344	22.882669340869725	22.44659191053575	26.697966997903183
27	26.934238915732216	21.6526878779591	24.769046099614066	26.644027106694622
28	26.002826146412616	23.14325219558149	21.98316467620871	28.870756981797186
29	27.191023186556052	23.697845443218768	22.452669644756433	26.658461725468747
30	26.65466314158082	21.97480779165527	24.082262132676938	27.288266934086973
31	27.919591576260373	22.550673109064938	22.971556203847207	26.558179110827485
32	25.475582702768406	22.23918923025496	22.973075637402378	29.312152429574255
33	25.402649892120216	22.22931291214635	25.022791503327557	27.34524569240587
34	26.305953140669157	21.90491384811742	24.01236818913909	27.77676482207433
35	25.186890327285987	24.304859148509436	24.88452304980703	25.623727474397544
36	26.00814416385571	22.708694198802686	25.052420457653383	26.23074117968821
37	27.10517519068891	22.4648251131978	24.347403288054213	26.08259640805908
38	25.818974686236974	21.893518096453644	25.505971373871823	26.781535843437567
39	25.09268544686541	20.95450815935819	26.063603488619442	27.889202905156957
40	24.87996474914152	22.0044367459811	27.247242228097367	25.86835627678002
41	23.145531345914243	20.906646002370316	28.16953839608594	27.778284255629504
42	21.763606527486555	21.258394870392316	29.251375087367432	27.7266235147537
43	20.839790925942808	22.284012520132496	28.79554502081624	28.08065153310846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	3.0
4	4.0
5	4.0
6	8.5
7	13.0
8	14.0
9	15.0
10	14.0
11	13.0
12	13.0
13	35.5
14	58.0
15	165.0
16	272.0
17	257.5
18	243.0
19	243.0
20	253.0
21	263.0
22	208.0
23	153.0
24	193.5
25	234.0
26	234.0
27	271.0
28	308.0
29	423.5
30	539.0
31	674.0
32	809.0
33	809.0
34	1038.5
35	1268.0
36	1436.0
37	1604.0
38	2136.0
39	2668.0
40	2668.0
41	3249.0
42	3830.0
43	4545.5
44	5261.0
45	7945.0
46	10629.0
47	10629.0
48	11898.5
49	13168.0
50	14980.5
51	16793.0
52	16993.0
53	17193.0
54	17193.0
55	15333.0
56	13473.0
57	12721.5
58	11970.0
59	10937.0
60	9904.0
61	9904.0
62	8774.0
63	7644.0
64	6131.0
65	4618.0
66	3959.0
67	3300.0
68	3300.0
69	2693.5
70	2087.0
71	1771.0
72	1455.0
73	1106.0
74	757.0
75	757.0
76	633.0
77	509.0
78	378.5
79	248.0
80	197.5
81	147.0
82	147.0
83	118.0
84	89.0
85	66.5
86	44.0
87	33.0
88	22.0
89	22.0
90	17.0
91	12.0
92	9.0
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	131628.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.68602425015955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.26338114723384	42.70975780229131
2	6.638487941604089	6.729571215850731
3	2.534586387277605	3.8540432126903093
4	1.4149317265464574	2.868690552162154
5	0.9577768784567651	2.427295104385085
6	0.632522445553607	1.9236028808460208
7	0.49762429365828803	1.7655817911082747
8	0.4226808759386663	1.7139210502324733
9	0.3072680126504489	1.4016774546449082
>10	2.0684383290615584	19.750357066885467
>50	0.15438344050242067	5.246604066004194
>100	0.10791852151625524	9.60889780289908
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	422	0.32060048014100345	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	419	0.31832132980824746	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	415	0.3152824626979062	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	377	0.2864132251496642	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	356	0.2704591728203726	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	340	0.2583037043790075	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	318	0.24158993527213052	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	312	0.23703163460661864	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	298	0.22639559972042422	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	289	0.21955814872215637	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	274	0.20816239705837664	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	260	0.19752636217218222	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	238	0.18081259306530525	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	223	0.1694168414015255	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	212	0.16105995684808702	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	204	0.1549822226274045	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	194	0.14738505485155134	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	191	0.14510590451879538	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	189	0.14358647096362476	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	188	0.14282675418603943	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	185	0.1405476038532835	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	180	0.1367490199653569	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	177	0.13446986963260096	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	177	0.13446986963260096	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	174	0.13219071929984502	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	174	0.13219071929984502	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	161	0.12231440119123592	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	154	0.1169963837481387	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	152	0.11547695019296807	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	151	0.11471723341538276	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	149	0.11319779986021211	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	149	0.11319779986021211	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	148	0.11243808308262679	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	147	0.11167836630504148	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	145	0.11015893274987085	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	143	0.10863949919470023	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	142	0.10787978241711489	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	141	0.10712006563952958	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	141	0.10712006563952958	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	139	0.10560063208435895	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	135	0.1025617649740177	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	133	0.10104233141884705	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	133	0.10104233141884705	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	133	0.10104233141884705	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	133	0.10104233141884705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	7.597167775853162E-4	0.0	0.0	0.0	0.0
9	7.597167775853162E-4	0.0	0.0	0.0	0.0
10	0.0015194335551706325	0.0	0.0	0.0	0.0
11	0.0022791503327559484	0.0	0.0	0.0	0.0
12	0.0022791503327559484	0.0	0.0	0.0	0.0
13	0.0022791503327559484	0.0	0.0	0.0	0.0
14	0.0022791503327559484	0.0	0.0	0.0	0.0
15	0.0022791503327559484	0.0	0.0	0.0	0.0
16	0.0022791503327559484	0.0	0.0	0.0	0.0
17	0.0022791503327559484	0.0	0.0	7.597167775853162E-4	0.0
18	0.0022791503327559484	0.0	0.0	0.0015194335551706325	0.0
19	0.0022791503327559484	0.0	0.0	0.0015194335551706325	0.0
20	0.0022791503327559484	0.0	0.0	0.003038867110341265	0.0
21	0.003038867110341265	0.0	0.0	0.007597167775853162	0.0
22	0.003038867110341265	0.0	0.0	0.014434618774121008	0.0
23	0.003038867110341265	0.0	0.0	0.01595405232929164	0.0
24	0.003038867110341265	0.0	0.0	0.01975263621721822	0.0
25	0.003038867110341265	0.0	0.0	0.021272069772388852	0.0
26	0.003038867110341265	0.0	0.0	0.02431093688273012	0.0
27	0.003038867110341265	0.0	0.0	0.044063573099948336	0.0
28	0.003038867110341265	0.0	0.0	0.1169963837481387	0.0
29	0.003038867110341265	0.0	0.0	0.25146625338073963	0.0
30	0.003038867110341265	0.0	0.0	0.4224025283374358	0.0
31	0.003038867110341265	0.0	0.0	0.8463244902300422	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCT	50	7.228664E-9	33.300003	30
ATACGGC	45	1.0676558E-7	32.88889	29
AGTCGGT	30	3.582335E-4	30.833334	11
GGTATCA	205	0.0	30.682928	1
CTATGCC	25	0.005478894	29.6	4
GGCCGTT	25	0.005478894	29.6	14
TTCGTGT	25	0.005478894	29.6	16
AAGTTCG	25	0.005478894	29.6	28
ACGAAAG	25	0.005478894	29.6	19
CTCTATG	65	2.9394869E-9	28.461538	1
CAGTCGG	35	8.827555E-4	26.42857	10
TCTATGG	70	6.4792403E-9	26.42857	2
AGCCGCG	275	0.0	24.218184	19
GCGGTAA	275	0.0	24.218184	23
AACTCCG	275	0.0	24.218184	5
CGTGCCA	280	0.0	23.785713	10
GCCAGCA	280	0.0	23.785713	13
GTGCCAG	280	0.0	23.785713	11
ACGGTGT	55	1.8861148E-5	23.545454	8
GGTAATA	285	0.0	23.368422	25
>>END_MODULE