FastQCFastQC Report
Fri 10 Feb 2017
ERR1631863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18727
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA610.32573289902280134No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA540.2883537138890372No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA480.25631441234581087No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA470.25097452875527315No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA450.24029476157419769No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC390.20825546003097134No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC350.18689592566882043No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT350.18689592566882043No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT320.17087627489720725No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG290.15485662412559406No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC250.13349708976344316No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC240.12815720617290544No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA230.1228173225823677No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA230.1228173225823677No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT220.11747743899182998No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT220.11747743899182998No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC210.11213755540129225No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA210.11213755540129225No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA210.11213755540129225No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA210.11213755540129225No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT200.10679767181075452No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA200.10679767181075452No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA200.10679767181075452No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC200.10679767181075452No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA190.10145778822021678No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT190.10145778822021678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGAC200.001796027237.037
GGTATCA401.4916441E-632.3751
GTATCAA352.2660206E-531.7142852
CCGCGGC250.00536089829.69
TATCAAC554.8879464E-420.1818183
TCAACGC608.7822205E-418.55
ATCAACG608.7822205E-418.54
AACGCAG650.001502797717.0769237
ACGCAGA650.001502797717.0769238
CAACGCA650.001502797717.0769236
CAGAGTA700.002466935715.85714211
AGAGTAC700.002466935715.85714212
GCAGAGT700.002466935715.85714210
CGCAGAG700.002466935715.8571429
TCTCTTA800.0059984713.87500137
TGTCTCT1350.001268572610.96296337
CTGTCTC1900.00210968.76315837
Produced by FastQC (version 0.11.5)