##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631863.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18727
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47578362791691	33.0	31.0	34.0	31.0	34.0
2	32.64607251561916	34.0	31.0	34.0	31.0	34.0
3	32.73952047845357	34.0	31.0	34.0	31.0	34.0
4	36.202595183425004	37.0	35.0	37.0	35.0	37.0
5	36.11144337053452	37.0	35.0	37.0	35.0	37.0
6	36.15880813798259	37.0	36.0	37.0	35.0	37.0
7	36.17087627489721	37.0	36.0	37.0	35.0	37.0
8	36.111069578683185	37.0	36.0	37.0	35.0	37.0
9	37.92817856570727	39.0	38.0	39.0	35.0	39.0
10	37.86548833235435	39.0	38.0	39.0	35.0	39.0
11	37.95509157900358	39.0	38.0	39.0	35.0	39.0
12	37.88946440967587	39.0	38.0	39.0	35.0	39.0
13	37.94083408981684	39.0	38.0	39.0	35.0	39.0
14	39.30426656698884	40.0	39.0	41.0	36.0	41.0
15	39.28418860468842	40.0	39.0	41.0	36.0	41.0
16	39.255727025150854	40.0	39.0	41.0	36.0	41.0
17	39.228653815346824	40.0	39.0	41.0	36.0	41.0
18	39.23863939766113	40.0	39.0	41.0	36.0	41.0
19	39.24590163934426	40.0	39.0	41.0	36.0	41.0
20	39.22630426656699	40.0	39.0	41.0	36.0	41.0
21	39.213541944785604	40.0	39.0	41.0	36.0	41.0
22	39.16254605649597	40.0	39.0	41.0	36.0	41.0
23	39.09350136167031	40.0	39.0	41.0	36.0	41.0
24	39.09307417098307	40.0	39.0	41.0	36.0	41.0
25	39.09350136167031	40.0	39.0	41.0	36.0	41.0
26	38.9761307203503	40.0	39.0	41.0	35.0	41.0
27	38.87974582154109	40.0	38.0	41.0	35.0	41.0
28	38.86570192769798	40.0	38.0	41.0	35.0	41.0
29	38.85892027553799	40.0	38.0	41.0	35.0	41.0
30	38.83141987504673	40.0	38.0	41.0	35.0	41.0
31	38.76872964169381	40.0	38.0	41.0	35.0	41.0
32	38.6941848667699	40.0	38.0	41.0	35.0	41.0
33	38.6534415549741	40.0	38.0	41.0	35.0	41.0
34	38.584557056656166	40.0	38.0	41.0	35.0	41.0
35	38.50921129919368	40.0	38.0	41.0	34.0	41.0
36	38.51268222352753	40.0	38.0	41.0	34.0	41.0
37	38.46344849682277	40.0	38.0	41.0	34.0	41.0
38	38.405510759865436	40.0	38.0	41.0	34.0	41.0
39	38.309820045923	40.0	38.0	41.0	34.0	41.0
40	38.007102045175415	40.0	37.0	41.0	33.0	41.0
41	38.10706466599028	40.0	37.0	41.0	33.0	41.0
42	38.12548726437764	40.0	37.0	41.0	34.0	41.0
43	37.63565974261761	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	3.0
22	3.0
23	8.0
24	18.0
25	15.0
26	28.0
27	40.0
28	66.0
29	106.0
30	146.0
31	200.0
32	298.0
33	374.0
34	505.0
35	735.0
36	1158.0
37	2021.0
38	4934.0
39	8067.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.43605489400331	16.948790516366742	13.066695146045818	27.54845944358413
2	20.24883857531906	20.10466171837454	32.73348640999626	26.91301329631014
3	20.793506701553905	20.54787205638917	29.743151599295135	28.91546964276179
4	15.426923693063491	15.715277406952529	35.099054840604474	33.7587440593795
5	15.875473914668662	33.700005339883596	34.12719602712661	16.29732471832114
6	33.924280450686176	34.68254392054253	15.448283227425641	15.944892401345651
7	29.422758583862873	28.797992203769958	20.07262241683131	21.706626795535858
8	27.035830618892508	30.490735301970417	21.380893896513058	21.09254018262402
9	28.03438885032306	13.232231537352485	17.578896780050197	41.15448283227426
10	18.764350937149572	24.478026379024936	29.73247183211406	27.025150851711434
11	38.67677684626475	19.672131147540984	19.058044534629147	22.59304747156512
12	22.347412826400383	24.040155924600846	26.886313878357456	26.726117370641322
13	34.67720403695199	18.29444118118225	20.686709029743152	26.341645752122606
14	24.365888823623642	19.581353126501842	23.020238158808137	33.03251989106638
15	28.579056976557908	24.89453729908688	20.307577294814973	26.218828429540235
16	25.61542158380947	25.17221124579484	21.58380947295349	27.628557697442197
17	27.48438084049768	24.35520905644257	21.02312169594703	27.137288407112724
18	26.437763656752285	21.573129705772416	23.511507449137607	28.47759918833769
19	28.434880119613393	22.934800021359532	23.31393175628771	25.316388102739364
20	29.812570085972123	20.937683558498424	22.956159555721687	26.293586799807766
21	26.854274576814223	22.40081166230576	22.17119666791264	28.57371709296737
22	28.221284775991883	23.06295722753244	21.14059913493886	27.57515886153682
23	27.66059699898542	22.678485609013723	22.33673305921931	27.32418433278155
24	28.36012174934586	21.770705398622308	22.774603513643402	27.094569338388425
25	27.85283280824478	22.52362898488813	22.78528328082448	26.83825492604261
26	27.70331606770972	23.212473968067496	21.738666097079083	27.345543867143697
27	26.266887381855074	22.56634805361243	24.200352432316976	26.966412132215517
28	26.16008971004432	23.223153735248573	22.480909916163828	28.135846638543278
29	26.44844342393336	24.034816041010306	22.21391573663694	27.302824798419394
30	26.261547498264537	22.19255620227479	24.510065680568164	27.035830618892508
31	28.130506754952744	23.452768729641694	22.641106423879958	25.775618091525605
32	25.262989266833983	23.212473968067496	22.35809259358146	29.166444171517057
33	26.11203075772948	22.272654456132855	24.33384952208042	27.281465264057243
34	26.2989266833983	21.904202488385753	24.440647193891174	27.35622363432477
35	25.481924494046034	23.928018369199552	24.80375927804774	25.786297858706682
36	25.95183425001335	22.822662465958242	24.702301489827523	26.523201794200883
37	26.98777166657767	23.04159769317029	23.85859988252256	26.11203075772948
38	25.61542158380947	22.61440700592727	25.017354621669245	26.752816788594007
39	24.94259625140172	21.519730869867036	25.668820419714848	27.86885245901639
40	24.451326961072247	22.496929566935442	27.29214503123832	25.75959844075399
41	23.37267047578363	21.30079564265499	28.077107919047368	27.249425962514017
42	22.155177017141025	21.74400598066962	28.579056976557908	27.52176002563144
43	21.43963261600897	22.24061515458963	28.429540236022856	27.890211993378543
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	1.5
11	2.0
12	2.0
13	3.5
14	5.0
15	14.0
16	23.0
17	20.5
18	18.0
19	18.0
20	25.5
21	33.0
22	25.5
23	18.0
24	26.0
25	34.0
26	34.0
27	50.0
28	66.0
29	94.0
30	122.0
31	149.0
32	176.0
33	176.0
34	196.0
35	216.0
36	265.5
37	315.0
38	430.5
39	546.0
40	546.0
41	647.0
42	748.0
43	807.0
44	866.0
45	1129.0
46	1392.0
47	1392.0
48	1586.0
49	1780.0
50	2002.0
51	2224.0
52	2219.5
53	2215.0
54	2215.0
55	2004.0
56	1793.0
57	1650.5
58	1508.0
59	1406.0
60	1304.0
61	1304.0
62	1203.0
63	1102.0
64	919.0
65	736.0
66	620.0
67	504.0
68	504.0
69	427.5
70	351.0
71	288.5
72	226.0
73	190.0
74	154.0
75	154.0
76	127.5
77	101.0
78	79.0
79	57.0
80	48.5
81	40.0
82	40.0
83	31.0
84	22.0
85	18.5
86	15.0
87	11.5
88	8.0
89	8.0
90	5.5
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	18727.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.56223634324772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.02090592334496	64.01452448336626
2	6.823461091753774	10.038981150210926
3	2.3664343786295006	5.222406151545896
4	1.2848432055749128	3.78063758210071
5	0.6533101045296167	2.402947615741977
6	0.5516840882694541	2.4349869172852032
7	0.3048780487804878	1.5699257756180913
8	0.19599303135888502	1.1534148555561488
9	0.16695702671312426	1.1053559032413094
>10	0.6170150987224158	7.662732952421638
>50	0.014518002322880372	0.6140866129118385
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	61	0.32573289902280134	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	54	0.2883537138890372	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	48	0.25631441234581087	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	47	0.25097452875527315	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	45	0.24029476157419769	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	39	0.20825546003097134	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	35	0.18689592566882043	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	35	0.18689592566882043	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	32	0.17087627489720725	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	29	0.15485662412559406	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	25	0.13349708976344316	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	24	0.12815720617290544	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	23	0.1228173225823677	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	23	0.1228173225823677	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	22	0.11747743899182998	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	22	0.11747743899182998	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	21	0.11213755540129225	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	21	0.11213755540129225	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	21	0.11213755540129225	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	21	0.11213755540129225	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	20	0.10679767181075452	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	20	0.10679767181075452	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	20	0.10679767181075452	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	20	0.10679767181075452	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	19	0.10145778822021678	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	19	0.10145778822021678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.010679767181075453	0.0
25	0.0	0.0	0.0	0.010679767181075453	0.0
26	0.0	0.0	0.0	0.010679767181075453	0.0
27	0.0	0.0	0.0	0.026699417952688632	0.0
28	0.0	0.0	0.0	0.1014577882202168	0.0
29	0.0	0.0	0.0	0.1868959256688204	0.0
30	0.0	0.0	0.0	0.3257328990228013	0.0
31	0.0	0.0	0.0	0.7475837026752816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAGAC	20	0.0017960272	37.0	37
GGTATCA	40	1.4916441E-6	32.375	1
GTATCAA	35	2.2660206E-5	31.714285	2
CCGCGGC	25	0.005360898	29.6	9
TATCAAC	55	4.8879464E-4	20.181818	3
TCAACGC	60	8.7822205E-4	18.5	5
ATCAACG	60	8.7822205E-4	18.5	4
AACGCAG	65	0.0015027977	17.076923	7
ACGCAGA	65	0.0015027977	17.076923	8
CAACGCA	65	0.0015027977	17.076923	6
CAGAGTA	70	0.0024669357	15.857142	11
AGAGTAC	70	0.0024669357	15.857142	12
GCAGAGT	70	0.0024669357	15.857142	10
CGCAGAG	70	0.0024669357	15.857142	9
TCTCTTA	80	0.00599847	13.875001	37
TGTCTCT	135	0.0012685726	10.962963	37
CTGTCTC	190	0.0021096	8.763158	37
>>END_MODULE