FastQCFastQC Report
Fri 10 Feb 2017
ERR1631862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815434
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19320.23692904637285175No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18800.23055207411022843No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16890.207128964453285No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16010.19633716523961472No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15560.19081863155080608No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15460.1895922907310708No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15340.1881206817473885No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13490.16543337658228624No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12800.15697162492611297No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12670.15537738186045713No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12490.15316996838493366No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12080.14814197102401913No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10060.12336988646536691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.1133138917435378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8770.10755008989078209No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8690.10656901723499387No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8650.10607848090709979No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8550.10485214008736451No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8270.10141838579210581No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8250.10117311762815875No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8180.10031467905434407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC200.001841622637.06
TCCTATA250.00549541729.62
GGTATCA11200.028.741071
TGCAACG400.001930708923.12512
CGTTAGA705.098924E-621.1428571
ACTGCGG450.003824868520.5555578
CGTGATA450.003824868520.55555725
CTTATAC7400.019.25000237
ATACGGC1551.8189894E-1219.09677529
GTTCTAG500.007033169718.51
GTACATT500.007033169718.51
CACGGAA500.007033169718.521
ATTGGTC500.007033169718.58
TTCGCTA701.2186461E-418.526
AGTACTC500.007033169718.55
GTATCAA17450.018.3409752
TACGGCT1653.6379788E-1217.93939430
GCGGTAA7400.017.75000223
TAATACG7250.017.60689727
CCGTATA852.7215438E-517.4117649
Produced by FastQC (version 0.11.5)