FastQCFastQC Report
Fri 10 Feb 2017
ERR1631861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521385
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA19820.3801413542775492No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18000.34523432779999425No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12930.24799332546966255No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11860.22747106265044065No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11320.21711403281644082No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10440.20023591012399666No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10030.1923722393241079No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9760.187193724407108No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9130.1751105229341082No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8840.16954841431955273No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8840.16954841431955273No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8710.16705505528544165No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8630.16552068049521948No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8520.16341091515866393No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8280.15880779078799734No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8250.15823240024166402No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8150.1563144317538863No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8070.1547800569636641No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8060.1545882601148863No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8050.15439646326610854No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8010.15362927587099745No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7980.15305388532466413No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7790.1494097451978864No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7580.14538201137355314No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC7580.14538201137355314No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7510.1440394334321087No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7410.14212146494433098No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7270.1394363090614421No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7210.1382855279687755No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7180.13771013742244215No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7120.1365593563297755No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7060.13540857523710884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6880.1319562319591089No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6840.1311890445639978No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6830.13099724771522003No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6820.13080545086644224No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6740.12927107607622007No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6610.126777717042109No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6480.12428435800799792No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6480.12428435800799792No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6460.12390076431044238No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC6430.12332537376410904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.12217459267144241No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA6080.11661248405688694No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6030.11565349981299806No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5970.11450271872033144No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5850.11220115653499813No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5780.11085857859355372No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5700.10932420380333151No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5660.10855701640822041No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5610.10759803216433153No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5600.10740623531555375No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5580.10702264161799822No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT5320.10203592354977609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.031.9345231
TCCCCGA250.005493623729.59999829
CCGTTAA405.9339996E-527.7516
AGCGCTG358.862868E-426.42857237
CTTAGTG358.862868E-426.4285723
TAACCCT856.91216E-1126.11764735
ATACGGC1800.025.69444529
TACGGCT1850.025.030
CGTTAAA451.3218577E-424.66666617
GGGATCC451.3218577E-424.66666619
CCGCTTA701.9187246E-723.78571525
TAGCGAA551.9000747E-523.54545410
GTTAGAA551.9000747E-523.5454542
TAAGGTA551.9000747E-523.5454545
GCTAAAC551.9000747E-523.5454543
ACGGCTG2050.023.46341331
GCTTATT802.7186616E-823.12527
TATTAAT400.001929779523.1252
CATACTG400.001929779523.1255
CTACCGT400.001929779523.1258
Produced by FastQC (version 0.11.5)