##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631861.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521385
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56011968123364	34.0	31.0	34.0	31.0	34.0
2	32.732245845200765	34.0	31.0	34.0	31.0	34.0
3	32.827373246257565	34.0	31.0	34.0	31.0	34.0
4	36.24609070072979	37.0	37.0	37.0	35.0	37.0
5	36.173652866883394	37.0	35.0	37.0	35.0	37.0
6	36.234377667174925	37.0	36.0	37.0	35.0	37.0
7	36.23493771397336	37.0	37.0	37.0	35.0	37.0
8	36.211034072710184	37.0	36.0	37.0	35.0	37.0
9	38.05162403981703	39.0	38.0	39.0	35.0	39.0
10	37.980101076939306	39.0	38.0	39.0	35.0	39.0
11	38.06406590139724	39.0	38.0	39.0	37.0	39.0
12	37.976366792293604	39.0	38.0	39.0	35.0	39.0
13	38.04745245835611	39.0	38.0	39.0	35.0	39.0
14	39.39820669946393	40.0	39.0	41.0	37.0	41.0
15	39.416573165702886	40.0	39.0	41.0	37.0	41.0
16	39.36205299346932	40.0	39.0	41.0	36.0	41.0
17	39.34015554724436	40.0	39.0	41.0	36.0	41.0
18	39.374619523001236	40.0	39.0	41.0	36.0	41.0
19	39.39910430871621	40.0	39.0	41.0	37.0	41.0
20	39.40115845296662	40.0	39.0	41.0	37.0	41.0
21	39.35679775981281	40.0	39.0	41.0	36.0	41.0
22	39.327107607622004	40.0	39.0	41.0	36.0	41.0
23	39.25731657028875	40.0	39.0	41.0	36.0	41.0
24	39.27484296633006	40.0	39.0	41.0	36.0	41.0
25	39.21763380227663	40.0	39.0	41.0	36.0	41.0
26	39.156930099638465	40.0	39.0	41.0	36.0	41.0
27	39.00268707385138	40.0	39.0	41.0	35.0	41.0
28	39.01203525226081	40.0	39.0	41.0	35.0	41.0
29	38.999625996144886	40.0	39.0	41.0	35.0	41.0
30	38.96931250419556	40.0	38.0	41.0	35.0	41.0
31	38.87761634876339	40.0	38.0	41.0	35.0	41.0
32	38.82886542574106	40.0	38.0	41.0	35.0	41.0
33	38.772258503792784	40.0	38.0	41.0	35.0	41.0
34	38.74998321777573	40.0	38.0	41.0	35.0	41.0
35	38.67554494279659	40.0	38.0	41.0	35.0	41.0
36	38.61802506784814	40.0	38.0	41.0	35.0	41.0
37	38.58319284214161	40.0	38.0	41.0	35.0	41.0
38	38.53948425827364	40.0	38.0	41.0	35.0	41.0
39	38.44900409486272	40.0	38.0	41.0	34.0	41.0
40	38.133062899776554	40.0	37.0	41.0	34.0	41.0
41	38.20514590945271	40.0	37.0	41.0	34.0	41.0
42	38.187120841604575	40.0	37.0	41.0	34.0	41.0
43	37.73562722364471	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	0.0
19	4.0
20	8.0
21	32.0
22	62.0
23	125.0
24	239.0
25	424.0
26	677.0
27	1086.0
28	1678.0
29	2501.0
30	3627.0
31	4891.0
32	6702.0
33	9103.0
34	13396.0
35	20180.0
36	30880.0
37	56192.0
38	128164.0
39	241410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20147299979861	16.7181641205635	12.609875619743569	27.470487259894323
2	21.52459315093453	20.099926158213222	32.21841825138813	26.15706243946412
3	20.998302597888316	19.87648282938711	30.01870019275583	29.10651437996874
4	14.679939008602089	16.35106495200284	36.66657076824228	32.3024252711528
5	16.053396242699733	33.53951494576944	34.12315275660021	16.283936054930617
6	31.781313233023585	35.8232400241664	16.247111059965285	16.14833568284473
7	28.855068711221076	28.485284386777526	20.32394487758566	22.33570202441574
8	26.26216711259434	30.431447011325602	21.433873241462642	21.872512634617415
9	27.89013876502009	12.685443578162012	18.03657565906192	41.38784199775598
10	19.438418826778676	22.905722258983285	29.747115854886502	27.908743059351536
11	39.08551262502757	19.662629342999892	19.15609386537779	22.09576416659474
12	21.978192698293967	24.468291185975815	27.448047028587318	26.105469087142897
13	35.89497204560929	17.615773372843485	20.618928431005877	25.870326150541345
14	23.404777659503054	20.65057491105421	24.10234279850782	31.842304630934915
15	29.632229542468618	24.141661152507265	20.636381944244654	25.589727360779463
16	24.081053348293487	24.694227873836034	23.209912061144834	28.014806716725644
17	26.111606586303786	23.839964709379824	22.14179541030141	27.90663329401498
18	26.26983898654545	21.11280531660865	23.90517563796427	28.712180058881632
19	27.69277980762776	22.689567210410733	23.81714088437527	25.800512097586235
20	30.292777889659273	20.658822175551656	22.85259453187184	26.195805402917234
21	27.4309771090461	22.030745034859077	21.9856727753963	28.552605080698523
22	28.059303585642088	23.196869875427947	21.419872071501867	27.323954467428102
23	28.1791766161282	22.05893917162941	22.464781303643182	27.297102908599214
24	27.523998580703317	21.34948262800042	23.141056992433615	27.985461798862644
25	28.03226022996442	22.26914851788985	23.3474304017185	26.35116085042723
26	27.953623521965532	22.655043777630734	22.70644533310318	26.684887367300554
27	26.45012802439656	22.12357470966752	25.022584078943584	26.403713186992338
28	26.14593822223501	23.55092685827172	22.36773209816163	27.935402821331646
29	26.69505260028578	24.28493339854426	22.55032269819807	26.469691302971892
30	26.732069392099888	22.557419181602846	24.321758393509597	26.38875303278767
31	27.95899383373131	22.92260038167573	22.863526952252176	26.254878832340783
32	24.842295041092473	22.779136338789954	23.365842899201166	29.012725720916404
33	25.275372325632688	22.29849343575285	25.313539898539467	27.112594340074992
34	25.587617595442907	22.190320013042186	24.661430612695035	27.56063177881987
35	25.154156717205133	24.35321307670915	24.977511819480807	25.515118386604907
36	25.841173029527127	23.17270347248195	25.240465299155133	25.74565819883579
37	26.88512327742455	22.96728904744095	24.23679238950104	25.91079528563346
38	25.429960585747573	22.286218437431074	25.413849650450242	26.869971326371108
39	24.778426690449475	21.224239285748535	26.483500676083892	27.513833347718098
40	24.614248587895702	22.116478226262743	27.542411078185985	25.726862107655574
41	23.402284300468942	21.125080314930425	28.022670387525533	27.4499649970751
42	21.491412296095973	21.837030217593526	29.753636947744948	26.917920538565554
43	20.78329833040843	22.19875907438841	28.898031205347298	28.11991138985587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.5
2	4.0
3	12.0
4	20.0
5	20.0
6	33.0
7	46.0
8	49.0
9	52.0
10	86.5
11	121.0
12	121.0
13	250.5
14	380.0
15	738.5
16	1097.0
17	1070.0
18	1043.0
19	1043.0
20	1249.5
21	1456.0
22	1236.5
23	1017.0
24	1203.0
25	1389.0
26	1389.0
27	1681.0
28	1973.0
29	2429.5
30	2886.0
31	3532.5
32	4179.0
33	4179.0
34	5468.5
35	6758.0
36	7612.5
37	8467.0
38	10165.0
39	11863.0
40	11863.0
41	14590.0
42	17317.0
43	20548.0
44	23779.0
45	32912.0
46	42045.0
47	42045.0
48	46685.0
49	51325.0
50	56200.5
51	61076.0
52	61568.0
53	62060.0
54	62060.0
55	56870.5
56	51681.0
57	48862.5
58	46044.0
59	43028.0
60	40012.0
61	40012.0
62	35546.0
63	31080.0
64	24385.5
65	17691.0
66	15101.0
67	12511.0
68	12511.0
69	10533.5
70	8556.0
71	7176.5
72	5797.0
73	4413.0
74	3029.0
75	3029.0
76	2485.5
77	1942.0
78	1509.0
79	1076.0
80	874.0
81	672.0
82	672.0
83	538.5
84	405.0
85	332.0
86	259.0
87	218.5
88	178.0
89	178.0
90	128.5
91	79.0
92	45.0
93	11.0
94	7.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	521385.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.81268083117625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.97160631971146	38.97430835379941
2	5.724831027188072	5.13090051267588
3	1.9924704039138978	2.6786382082847497
4	1.09265604526333	1.9585938645856351
5	0.6881839864298878	1.5419684668504543
6	0.5252196182968791	1.4121899472606159
7	0.36117695054558496	1.132971518586378
8	0.2980881733789124	1.0686504138542023
9	0.24273875789299842	0.9789997034533593
>10	1.702123226815443	15.365603648817489
>50	0.21530907119441345	6.770792204676444
>100	0.16148180339539786	14.462058625311473
>500	0.021100288976998654	6.702673253250009
>1k	0.0030143269967140935	1.8216512785938874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1982	0.3801413542775492	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1800	0.34523432779999425	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1293	0.24799332546966255	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1186	0.22747106265044065	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1132	0.21711403281644082	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1044	0.20023591012399666	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1003	0.1923722393241079	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	976	0.187193724407108	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	913	0.1751105229341082	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	884	0.16954841431955273	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	884	0.16954841431955273	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	871	0.16705505528544165	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	863	0.16552068049521948	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	852	0.16341091515866393	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	828	0.15880779078799734	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	825	0.15823240024166402	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	815	0.1563144317538863	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	807	0.1547800569636641	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	806	0.1545882601148863	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	805	0.15439646326610854	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	801	0.15362927587099745	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	798	0.15305388532466413	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	779	0.1494097451978864	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	758	0.14538201137355314	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	758	0.14538201137355314	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	751	0.1440394334321087	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	741	0.14212146494433098	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	727	0.1394363090614421	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	721	0.1382855279687755	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	718	0.13771013742244215	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	712	0.1365593563297755	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	706	0.13540857523710884	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	688	0.1319562319591089	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	684	0.1311890445639978	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	683	0.13099724771522003	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	682	0.13080545086644224	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	674	0.12927107607622007	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	661	0.126777717042109	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	648	0.12428435800799792	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	648	0.12428435800799792	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	646	0.12390076431044238	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	643	0.12332537376410904	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	637	0.12217459267144241	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	608	0.11661248405688694	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	603	0.11565349981299806	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	597	0.11450271872033144	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	585	0.11220115653499813	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	578	0.11085857859355372	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	570	0.10932420380333151	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	566	0.10855701640822041	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	561	0.10759803216433153	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	560	0.10740623531555375	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	558	0.10702264161799822	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	532	0.10203592354977609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.917968487777746E-4	0.0
2	0.0	0.0	0.0	1.917968487777746E-4	0.0
3	0.0	0.0	0.0	1.917968487777746E-4	0.0
4	0.0	0.0	0.0	1.917968487777746E-4	0.0
5	0.0	0.0	0.0	1.917968487777746E-4	0.0
6	0.0	0.0	0.0	1.917968487777746E-4	0.0
7	0.0	0.0	0.0	1.917968487777746E-4	0.0
8	0.0	0.0	0.0	1.917968487777746E-4	0.0
9	0.0	0.0	0.0	1.917968487777746E-4	0.0
10	0.0	0.0	0.0	1.917968487777746E-4	0.0
11	0.0	0.0	0.0	1.917968487777746E-4	0.0
12	0.0	0.0	0.0	1.917968487777746E-4	0.0
13	0.0	0.0	0.0	1.917968487777746E-4	0.0
14	0.0	0.0	0.0	3.835936975555492E-4	0.0
15	0.0	0.0	0.0	3.835936975555492E-4	0.0
16	0.0	0.0	0.0	0.0015343747902221967	0.0
17	0.0	0.0	0.0	0.0026851558828888443	0.0
18	1.917968487777746E-4	0.0	0.0	0.0038359369755554917	0.0
19	1.917968487777746E-4	0.0	0.0	0.005178514916999914	0.0
20	1.917968487777746E-4	0.0	0.0	0.005945702312111012	0.0
21	1.917968487777746E-4	0.0	0.0	0.009589842438888729	0.0
22	3.835936975555492E-4	0.0	0.0	0.01630273214611084	0.0
23	3.835936975555492E-4	0.0	0.0	0.018987888028999683	0.0
24	5.753905463333237E-4	0.0	0.0	0.0233992155508885	0.0
25	5.753905463333237E-4	0.0	0.0	0.024166402945999598	0.0
26	5.753905463333237E-4	0.0	0.0	0.02723515252644399	0.0
27	5.753905463333237E-4	0.0	0.0	0.04277069727744373	0.0
28	5.753905463333237E-4	0.0	0.0	0.1185304525446647	1.917968487777746E-4
29	5.753905463333237E-4	0.0	0.0	0.25317184038666246	1.917968487777746E-4
30	5.753905463333237E-4	0.0	0.0	0.4336526750865483	1.917968487777746E-4
31	5.753905463333237E-4	0.0	0.0	0.8582908982805413	1.917968487777746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	840	0.0	31.934523	1
TCCCCGA	25	0.0054936237	29.599998	29
CCGTTAA	40	5.9339996E-5	27.75	16
AGCGCTG	35	8.862868E-4	26.428572	37
CTTAGTG	35	8.862868E-4	26.428572	3
TAACCCT	85	6.91216E-11	26.117647	35
ATACGGC	180	0.0	25.694445	29
TACGGCT	185	0.0	25.0	30
CGTTAAA	45	1.3218577E-4	24.666666	17
GGGATCC	45	1.3218577E-4	24.666666	19
CCGCTTA	70	1.9187246E-7	23.785715	25
TAGCGAA	55	1.9000747E-5	23.545454	10
GTTAGAA	55	1.9000747E-5	23.545454	2
TAAGGTA	55	1.9000747E-5	23.545454	5
GCTAAAC	55	1.9000747E-5	23.545454	3
ACGGCTG	205	0.0	23.463413	31
GCTTATT	80	2.7186616E-8	23.125	27
TATTAAT	40	0.0019297795	23.125	2
CATACTG	40	0.0019297795	23.125	5
CTACCGT	40	0.0019297795	23.125	8
>>END_MODULE