FastQCFastQC Report
Fri 10 Feb 2017
ERR1631860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12585
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA470.37346046881207784No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA330.2622169249106079No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA240.1907032181168057No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT240.1907032181168057No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC230.18275725069527216No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA210.16686531585220502No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA210.16686531585220502No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG200.15891934843067143No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA200.15891934843067143No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA200.15891934843067143No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA180.1430274135876043No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC170.1350814461660707No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA170.1350814461660707No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT170.1350814461660707No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA150.11918951132300357No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT150.11918951132300357TruSeq Adapter, Index 3 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT140.11124354390147No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA140.11124354390147No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA140.11124354390147No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG130.10329757647993643No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA130.10329757647993643No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)