##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631860.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12585
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56336909018673	34.0	31.0	34.0	31.0	34.0
2	32.74231227651967	34.0	31.0	34.0	31.0	34.0
3	32.83114819229241	34.0	31.0	34.0	31.0	34.0
4	36.28112832737386	37.0	37.0	37.0	35.0	37.0
5	36.18959078267779	37.0	35.0	37.0	35.0	37.0
6	36.244576877234806	37.0	36.0	37.0	35.0	37.0
7	36.231466030989274	37.0	36.0	37.0	35.0	37.0
8	36.21899086213747	37.0	36.0	37.0	35.0	37.0
9	38.05331744139849	39.0	38.0	39.0	35.0	39.0
10	37.96082638061184	39.0	38.0	39.0	35.0	39.0
11	38.045530393325386	39.0	38.0	39.0	35.0	39.0
12	37.97902264600715	39.0	38.0	39.0	35.0	39.0
13	38.032101708382996	39.0	38.0	39.0	35.0	39.0
14	39.42526817640048	40.0	39.0	41.0	37.0	41.0
15	39.417163289630516	40.0	39.0	41.0	37.0	41.0
16	39.38529996027016	40.0	39.0	41.0	36.0	41.0
17	39.36344854986095	40.0	39.0	41.0	36.0	41.0
18	39.333492252681765	40.0	39.0	41.0	36.0	41.0
19	39.34119984108065	40.0	39.0	41.0	36.0	41.0
20	39.36360746920938	40.0	39.0	41.0	36.0	41.0
21	39.36583234008741	40.0	39.0	41.0	36.0	41.0
22	39.31378625347636	40.0	39.0	41.0	36.0	41.0
23	39.239809296781885	40.0	39.0	41.0	36.0	41.0
24	39.269368295589985	40.0	39.0	41.0	36.0	41.0
25	39.1808502185141	40.0	39.0	41.0	36.0	41.0
26	39.1222884386174	40.0	39.0	41.0	36.0	41.0
27	39.02185141040922	40.0	39.0	41.0	35.0	41.0
28	39.05681366706396	40.0	39.0	41.0	35.0	41.0
29	39.03194278903457	40.0	39.0	41.0	35.0	41.0
30	38.992371871275324	40.0	39.0	41.0	35.0	41.0
31	38.87922129519269	40.0	38.0	41.0	35.0	41.0
32	38.85299960270163	40.0	38.0	41.0	35.0	41.0
33	38.779419944378226	40.0	38.0	41.0	35.0	41.0
34	38.72077870480731	40.0	38.0	41.0	35.0	41.0
35	38.62701628923321	40.0	38.0	41.0	35.0	41.0
36	38.59133889551053	40.0	38.0	41.0	35.0	41.0
37	38.58816050854192	40.0	38.0	41.0	35.0	41.0
38	38.49781485895908	40.0	38.0	41.0	34.0	41.0
39	38.447596344854986	40.0	38.0	41.0	34.0	41.0
40	38.08406833531983	40.0	37.0	41.0	34.0	41.0
41	38.14572904251093	40.0	37.0	41.0	34.0	41.0
42	38.186650774731824	40.0	37.0	41.0	34.0	41.0
43	37.71736193881605	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	3.0
24	7.0
25	8.0
26	6.0
27	28.0
28	50.0
29	64.0
30	81.0
31	110.0
32	185.0
33	218.0
34	351.0
35	492.0
36	729.0
37	1356.0
38	3067.0
39	5828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.146603098927294	15.780691299165673	12.896305125148988	28.176400476758047
2	21.414382201032975	18.974970202622167	31.887167262614224	27.72348033373063
3	21.152165276122368	19.793404847040126	28.780294000794598	30.274135876042905
4	15.86015097338101	16.050854191497816	33.055224473579656	35.033770361541514
5	16.694477552642034	32.761223678982915	33.96901072705602	16.57528804131903
6	36.13031386571315	32.79300754866905	15.12912197059992	15.947556615017877
7	30.941597139451726	27.62812872467223	19.785458879618595	21.64481525625745
8	28.001589193484307	30.34564958283671	20.53237981724275	21.120381406436234
9	29.177592371871274	12.371871275327772	17.711561382598333	40.73897497020263
10	18.386968613428685	23.73460468812078	30.123162495033775	27.75526420341677
11	39.01470003972984	19.22129519268971	18.649185538339292	23.11481922924116
12	22.78108859753675	23.718712753277714	26.285260230433057	27.21493841875248
13	35.24036551450139	17.30631704410012	20.333730631704412	27.11958680969408
14	24.600715137067937	19.51529598728645	22.375844259038537	33.50814461660707
15	29.908621374652366	24.02860548271752	19.22129519268971	26.841477949940405
16	26.32499006754072	23.559793404847042	22.39968216130314	27.715534366309097
17	27.334127930075486	23.559793404847042	21.342868494239173	27.7632101708383
18	26.91299165673421	21.215733015494635	22.876440206595152	28.994835121176006
19	28.533969010727056	21.978545887961857	22.47119586809694	27.01628923321414
20	30.43305522447358	20.500595947556615	22.789034564958282	26.27731426301152
21	28.200238379022647	21.303138657131505	21.509733810091376	28.98688915375447
22	28.52602304330552	22.79698053237982	20.945570123162497	27.73142630115217
23	28.47834723877632	21.438220103297574	21.414382201032975	28.669050456893125
24	28.00953516090584	22.081843464441796	21.994437822804926	27.914183551847437
25	28.637266587206994	21.875248311481922	22.391736193881606	27.095748907429478
26	28.716726261422327	23.281684545093366	21.366706396503773	26.63488279698053
27	27.405641636869287	21.922924116011124	22.74135876042908	27.9300754866905
28	26.436233611442194	22.64600715137068	22.288438617401667	28.62932061978546
29	28.23996821613031	22.661899086213747	21.414382201032975	27.683750496622967
30	27.365911799761623	21.80373460468812	23.11481922924116	27.715534366309097
31	28.406833531982517	22.288438617401667	21.962653953118792	27.34207389749702
32	26.45212554628526	22.852602304330553	21.454112038140643	29.24116011124354
33	27.07191100516488	22.375844259038537	23.885578069129917	26.666666666666668
34	27.24672228843862	22.153357171235598	23.059197457290427	27.540723083035363
35	26.491855383392927	23.575685339690107	24.00476758045292	25.927691696464045
36	26.52363925307906	23.00357568533969	23.869686134286848	26.6030989272944
37	27.707588398887566	21.684545093365116	23.49622566547477	27.111640842272543
38	26.030989272943984	21.93881605085419	24.799364322606277	27.230830353595547
39	26.5554231227652	21.263408820023837	24.71195868096941	27.46920937624156
40	25.729042510925705	21.84346444179579	26.332936034962255	26.09455701231625
41	24.21136273341279	21.191895113230036	26.841477949940405	27.75526420341677
42	22.630115216527614	21.565355582042116	27.079856972586413	28.72467222884386
43	21.62892332141438	22.391736193881606	27.977751291219704	28.001589193484307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.0
7	0.0
8	1.5
9	3.0
10	2.5
11	2.0
12	2.0
13	3.5
14	5.0
15	9.5
16	14.0
17	14.5
18	15.0
19	15.0
20	12.0
21	9.0
22	11.0
23	13.0
24	16.0
25	19.0
26	19.0
27	24.0
28	29.0
29	38.0
30	47.0
31	63.0
32	79.0
33	79.0
34	123.5
35	168.0
36	169.0
37	170.0
38	229.0
39	288.0
40	288.0
41	337.0
42	386.0
43	460.0
44	534.0
45	760.5
46	987.0
47	987.0
48	991.5
49	996.0
50	1198.0
51	1400.0
52	1424.5
53	1449.0
54	1449.0
55	1320.5
56	1192.0
57	1139.5
58	1087.0
59	1013.0
60	939.0
61	939.0
62	898.0
63	857.0
64	694.0
65	531.0
66	493.5
67	456.0
68	456.0
69	378.5
70	301.0
71	262.5
72	224.0
73	188.0
74	152.0
75	152.0
76	121.5
77	91.0
78	73.0
79	55.0
80	45.5
81	36.0
82	36.0
83	27.0
84	18.0
85	18.0
86	18.0
87	13.0
88	8.0
89	8.0
90	5.5
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12585.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.43702820818434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.09186999901215	72.46722288438617
2	5.47268596265929	8.804131903059197
3	1.9658204089696731	4.743742550655542
4	0.928578484638941	2.9876837504966227
5	0.345747308110244	1.3905442987683752
6	0.3259903190753729	1.5733015494636473
7	0.23708386841845303	1.3349225268176401
8	0.08890645065691989	0.5721096543504172
9	0.1382989232440976	1.00119189511323
>10	0.4050182752148573	5.125148986889154
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	47	0.37346046881207784	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	33	0.2622169249106079	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	24	0.1907032181168057	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	24	0.1907032181168057	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	23	0.18275725069527216	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	21	0.16686531585220502	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	21	0.16686531585220502	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	20	0.15891934843067143	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	20	0.15891934843067143	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	20	0.15891934843067143	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	18	0.1430274135876043	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	17	0.1350814461660707	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	17	0.1350814461660707	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	17	0.1350814461660707	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	15	0.11918951132300357	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	15	0.11918951132300357	TruSeq Adapter, Index 3 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	14	0.11124354390147	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	14	0.11124354390147	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	14	0.11124354390147	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	13	0.10329757647993643	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	13	0.10329757647993643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.007945967421533572	0.0
5	0.0	0.0	0.0	0.007945967421533572	0.0
6	0.0	0.0	0.0	0.007945967421533572	0.0
7	0.0	0.0	0.0	0.007945967421533572	0.0
8	0.0	0.0	0.0	0.007945967421533572	0.0
9	0.0	0.0	0.0	0.007945967421533572	0.0
10	0.0	0.0	0.0	0.007945967421533572	0.0
11	0.0	0.0	0.0	0.007945967421533572	0.0
12	0.0	0.0	0.0	0.007945967421533572	0.0
13	0.0	0.0	0.0	0.007945967421533572	0.0
14	0.0	0.0	0.0	0.007945967421533572	0.0
15	0.0	0.0	0.0	0.007945967421533572	0.0
16	0.0	0.0	0.0	0.007945967421533572	0.0
17	0.0	0.0	0.0	0.007945967421533572	0.0
18	0.0	0.0	0.0	0.007945967421533572	0.0
19	0.0	0.0	0.0	0.007945967421533572	0.0
20	0.0	0.0	0.0	0.007945967421533572	0.0
21	0.0	0.0	0.0	0.007945967421533572	0.0
22	0.0	0.0	0.0	0.007945967421533572	0.0
23	0.0	0.0	0.0	0.007945967421533572	0.0
24	0.0	0.0	0.0	0.015891934843067144	0.0
25	0.0	0.0	0.0	0.015891934843067144	0.0
26	0.0	0.0	0.0	0.015891934843067144	0.0
27	0.0	0.0	0.0	0.04767580452920143	0.0
28	0.0	0.0	0.0	0.07945967421533572	0.0
29	0.0	0.0	0.0	0.18275725069527216	0.0
30	0.0	0.0	0.0	0.2542709574890743	0.0
31	0.0	0.0	0.0	0.5800556217719507	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE