FastQCFastQC Report
Fri 10 Feb 2017
ERR1631859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115164
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6510.5652808169219548No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5500.4775797992428189No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5100.44284672293425026No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4730.4107186273488243No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4610.4002987044562537No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4560.3959570699176826No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4400.38206383939425514No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4340.3768538779479698No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4140.3594873397936855No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3930.341252474731687No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3780.3282275711159737No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3470.30130943697683304No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3000.26049807231426486No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2940.2552881108679796No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2820.24486818797540896No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2820.24486818797540896No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2800.24313153415998057No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2520.2188183807439825No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2510.21795005383626825No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2470.21447674620541143No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2420.21013511166684032No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2390.20753013094369768No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2370.20579347712826926No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2110.18321697752769964No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2100.18234865061998543No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2070.17974366989684276No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2010.17453370845055746No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1910.16585043937341531No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1900.1649821124657011No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1830.15890382411170156No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1820.15803549720398735No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1790.15543051648084472No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1790.15543051648084472No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1780.1545621895731305No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1770.15369386266541626No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1770.15369386266541626No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1740.15108888194227363No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1720.1493522281268452No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1660.1441422666805599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1650.1432739397728457No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1640.14240561286513145No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1640.14240561286513145No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1600.1389323052342746No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1590.13806397832656037No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG1560.13545899760341773No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1550.13459067069570352No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG1540.1337223437879893No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1540.1337223437879893No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1540.1337223437879893No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1530.1328540168802751No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1520.13198568997256088No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1520.13198568997256088No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1470.1276440554339898No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1470.1276440554339898No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1450.12590740161856137No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1450.12590740161856137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.12417074780313292No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1430.12417074780313292No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1380.11982911326456185No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1370.11896078635684763No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1360.11809245944913341No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1360.11809245944913341No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1350.1172241325414192No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1350.1172241325414192No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1350.1172241325414192No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1330.11548747872599076No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1330.11548747872599076No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG1330.11548747872599076No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1330.11548747872599076No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1320.11461915181827656No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1320.11461915181827656No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG1300.11288249800284812No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC1260.10940919037199125No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1260.10940919037199125No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1240.10767253655656281No Hit
GGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTC1240.10767253655656281No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1210.10506755583342016No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1210.10506755583342016No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC1200.10419922892570596No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1190.10333090201799172No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA1190.10333090201799172No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1170.10159424820256331No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1170.10159424820256331No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1170.10159424820256331No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1160.10072592129484909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCAT200.001835067237.06
GGCACAT200.001835067237.016
TGGGCAC200.001835067237.014
AGCTAAT401.583574E-632.3751
ACACAGC352.3698294E-531.7142871
GCTTATT303.5795756E-430.83333427
GGGCAGT850.030.4705877
CAAGAGG250.005476081729.630
AATCCCA250.005476081729.65
GCAAGAG250.005476081729.629
CATCTGG250.005476081729.610
TAAAAAG250.005476081729.634
ATGGCAA250.005476081729.626
TCAATGT250.005476081729.618
CCATCTG250.005476081729.69
AAGGCTC250.005476081729.613
AGGCTCA250.005476081729.614
TAATCCC250.005476081729.64
CTTAACG250.005476081729.62
CGTAAAA250.005476081729.632
Produced by FastQC (version 0.11.5)