FastQCFastQC Report
Fri 10 Feb 2017
ERR1631858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469704
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11930.2539897467341134No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11270.2399383441486553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10910.23227394273840546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.22333214109311397No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10120.2154548396436905No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10080.21460323948699606No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9930.21140973889939196No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9870.21013233866435033No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9760.20779043823344065No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9590.2041711375674893No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9270.1973583363139339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8600.18309403368930222No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8240.17542963227905234No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7920.1686168310254969No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7660.1630814300069831No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7000.14903002742152505No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6950.147965527225657No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6300.13412702467937254No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6260.13327542452267813No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6250.1330625244835045No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6110.130081923935074No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5780.12305622264234496No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5670.12071432221143528No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5580.11879822185887283No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5560.11837242178052562No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5250.1117725205661438No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5250.1117725205661438No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5180.11028222029192854No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5140.10943062013523411No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5130.10921772009606048No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5120.1090048200568869No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5010.10666291962597721No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4710.100275918450769No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4700.10006301841159537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.027.8453621
CTCTGGA405.9327675E-527.752
GCCTAGT509.076151E-625.9000021
CTATATC551.8995555E-523.5454544
TAGTTCC551.8995555E-523.5454544
GTACTAT551.8995555E-523.5454541
GATGCTA551.8995555E-523.54545414
TCGGGTA1054.0017767E-1122.9047625
ATGCTAG603.7202517E-521.58333215
AGTTCCG1151.3460522E-1020.91304232
CGTTAAA450.00382249120.55555517
GTTATAG450.00382249120.55555511
TAAGTTC1202.382876E-1020.04166630
CTTGTCG1303.274181E-1119.92307721
CTAGTTC656.891031E-519.9230773
TCCTTGT1303.274181E-1119.92307719
TCTCGTC759.246656E-619.73333412
TTCTCGT759.246656E-619.73333411
TCTAATA851.2421024E-619.5882362
ATCTAAT1052.2508175E-819.380951
Produced by FastQC (version 0.11.5)