##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631858.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469704
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54007630337404	34.0	31.0	34.0	31.0	34.0
2	32.70680896905285	34.0	31.0	34.0	31.0	34.0
3	32.79027217141008	34.0	31.0	34.0	31.0	34.0
4	36.22933379319742	37.0	37.0	37.0	35.0	37.0
5	36.15505296952975	37.0	35.0	37.0	35.0	37.0
6	36.215808253708715	37.0	36.0	37.0	35.0	37.0
7	36.21310442321121	37.0	36.0	37.0	35.0	37.0
8	36.1937347776472	37.0	36.0	37.0	35.0	37.0
9	38.02295914022448	39.0	38.0	39.0	35.0	39.0
10	37.94555081498135	39.0	38.0	39.0	35.0	39.0
11	38.02610367380308	39.0	38.0	39.0	35.0	39.0
12	37.954077461550256	39.0	38.0	39.0	35.0	39.0
13	38.00912276667859	39.0	38.0	39.0	35.0	39.0
14	39.361014596426685	40.0	39.0	41.0	37.0	41.0
15	39.37137644133327	40.0	39.0	41.0	36.0	41.0
16	39.30916279188595	40.0	39.0	41.0	36.0	41.0
17	39.29570963841057	40.0	39.0	41.0	36.0	41.0
18	39.32896675352988	40.0	39.0	41.0	36.0	41.0
19	39.339352017440774	40.0	39.0	41.0	36.0	41.0
20	39.34129792379882	40.0	39.0	41.0	36.0	41.0
21	39.29731064670516	40.0	39.0	41.0	36.0	41.0
22	39.271794577010205	40.0	39.0	41.0	36.0	41.0
23	39.2049162877046	40.0	39.0	41.0	36.0	41.0
24	39.21780099807538	40.0	39.0	41.0	36.0	41.0
25	39.16192538279427	40.0	39.0	41.0	36.0	41.0
26	39.09358659921993	40.0	39.0	41.0	36.0	41.0
27	38.98400907805767	40.0	39.0	41.0	35.0	41.0
28	38.96782015907891	40.0	38.0	41.0	35.0	41.0
29	38.960453817723504	40.0	38.0	41.0	35.0	41.0
30	38.89954098751554	40.0	38.0	41.0	35.0	41.0
31	38.808245192717116	40.0	38.0	41.0	35.0	41.0
32	38.754834959889635	40.0	38.0	41.0	35.0	41.0
33	38.69076908010151	40.0	38.0	41.0	35.0	41.0
34	38.659626062371196	40.0	38.0	41.0	35.0	41.0
35	38.57431701667433	40.0	38.0	41.0	35.0	41.0
36	38.52452821351319	40.0	38.0	41.0	35.0	41.0
37	38.4714288147429	40.0	38.0	41.0	35.0	41.0
38	38.423766882973105	40.0	38.0	41.0	34.0	41.0
39	38.341427792822714	40.0	38.0	41.0	34.0	41.0
40	37.998124350654884	40.0	37.0	41.0	34.0	41.0
41	38.067457377412154	40.0	37.0	41.0	34.0	41.0
42	38.03766840393099	40.0	37.0	41.0	34.0	41.0
43	37.57067003900329	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	6.0
19	7.0
20	13.0
21	49.0
22	60.0
23	149.0
24	227.0
25	432.0
26	635.0
27	1073.0
28	1605.0
29	2403.0
30	3462.0
31	4718.0
32	6259.0
33	8555.0
34	12733.0
35	18864.0
36	28818.0
37	52493.0
38	117083.0
39	210058.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.72690886175123	17.101834346737522	13.036720998756666	27.134535792754587
2	20.885919643009217	19.909560063359052	33.04293767990053	26.1615826137312
3	21.360686730366357	20.496525471360684	29.82176008720386	28.321027711069096
4	15.030742765656669	16.791213189583225	35.49128813039702	32.68675591436308
5	16.63451876075145	32.97566126752167	33.6473608911144	16.74245908061247
6	31.69698363224499	35.0235467443326	16.165925774530344	17.113543848892068
7	29.136434861104014	28.336143613850425	20.499931871987464	22.027489653058097
8	26.690639211077617	30.851557576686595	20.53207977790268	21.92572343433311
9	28.308892408836208	12.930696779248208	18.608953724047485	40.1514570878681
10	19.85356735305639	23.19865276855211	29.49857782773832	27.449202050653177
11	38.30774274862467	20.34919864425255	19.15419072437109	22.18886788275169
12	22.29212440175089	24.912710983938823	26.909713351387254	25.88545126292303
13	34.66182957777664	18.24681075741318	21.19611500008516	25.89524466472502
14	23.529499429427894	21.29830701888849	24.1360516410335	31.036141910650112
15	29.84752099194386	24.37024168412447	20.856326537564083	24.925910786367584
16	24.313397373665115	24.67128233951595	23.75347027063853	27.261850016180407
17	26.015533186858107	24.220785856624598	22.567617052441538	27.196063904075757
18	25.840955154735752	21.7628549043653	24.405157290548942	27.991032650350007
19	27.466659853865412	22.87398020881236	24.3885510874934	25.27080884982883
20	29.43470781598624	20.92339004990377	23.96956381039974	25.672338323710253
21	26.72001941648357	22.736659683545383	23.135208556878375	27.40811234309267
22	27.557568170592546	23.697477560335873	22.52120484390169	26.223749425169895
23	27.154761296476078	22.618713061843206	23.833520685367805	26.39300495631291
24	27.161148297651287	22.25146049426873	23.833946485446152	26.75344472263383
25	26.722148416875307	22.764762488716297	24.247824161599645	26.265264932808748
26	26.922487353737672	23.589324340435677	23.39984330557117	26.08834500025548
27	26.454533067634085	22.368342615775042	25.203319537410795	25.97380477918008
28	25.51521809480013	23.669587655204126	23.29360618602354	27.521588063972203
29	26.859468942142286	23.759644371774563	23.36514059918587	26.01574608689728
30	26.067906596494815	22.333639909389742	25.211835538977738	26.3866179551377
31	26.8803331459813	23.044513140190418	24.002350416432478	26.072803297395808
32	24.655101936538756	22.997036431454703	24.08644593190605	28.26141570010049
33	24.880988878101952	22.377071517381157	26.087919200177133	26.654020404339757
34	25.21545483964369	22.361955614599832	25.389394171648526	27.033195374107947
35	24.99829679968661	24.18799925059186	25.314879157937764	25.498824791783765
36	25.38130397015993	23.04940984109141	25.661906221790744	25.907379966957915
37	25.819878050857557	22.737298383662903	25.266337949006186	26.176485616473354
38	25.20417113756749	22.010670549963383	26.0285200892477	26.756638223221437
39	24.738771651933984	20.98066016044147	27.252482414456765	27.02808577316778
40	24.574838621770308	22.269982797676835	27.561613271336842	25.593565309216014
41	22.97595932757652	21.064968575954218	28.7372472876535	27.221824808815764
42	21.343228927154122	21.855679321444995	29.545628736395685	27.255463015005194
43	20.925306150256333	22.498637439749288	29.09853865413111	27.477517755863268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	6.5
2	4.0
3	22.5
4	41.0
5	41.0
6	64.0
7	87.0
8	93.5
9	100.0
10	155.0
11	210.0
12	210.0
13	435.0
14	660.0
15	1252.0
16	1844.0
17	1794.5
18	1745.0
19	1745.0
20	1953.0
21	2161.0
22	1634.0
23	1107.0
24	1133.0
25	1159.0
26	1159.0
27	1297.0
28	1435.0
29	1846.5
30	2258.0
31	2858.5
32	3459.0
33	3459.0
34	4763.5
35	6068.0
36	6921.5
37	7775.0
38	10144.5
39	12514.0
40	12514.0
41	14760.5
42	17007.0
43	20532.5
44	24058.0
45	31765.5
46	39473.0
47	39473.0
48	43905.5
49	48338.0
50	53601.0
51	58864.0
52	57803.5
53	56743.0
54	56743.0
55	51060.5
56	45378.0
57	42082.0
58	38786.0
59	34596.0
60	30406.0
61	30406.0
62	26745.5
63	23085.0
64	18650.5
65	14216.0
66	12215.0
67	10214.0
68	10214.0
69	8681.5
70	7149.0
71	5938.5
72	4728.0
73	3814.0
74	2900.0
75	2900.0
76	2416.5
77	1933.0
78	1588.0
79	1243.0
80	1113.0
81	983.0
82	983.0
83	825.5
84	668.0
85	536.5
86	405.0
87	345.5
88	286.0
89	286.0
90	208.0
91	130.0
92	96.0
93	62.0
94	36.5
95	11.0
96	11.0
97	6.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	469704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.1237747621421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.80871073683812	33.22598057948271
2	7.2415171902402085	5.811140093547564
3	2.739541381429999	3.2976222402019473
4	1.5115657347811755	2.425988923221268
5	0.9756545751154455	1.957347220879279
6	0.6687123524030617	1.6098758285089585
7	0.5163001991912393	1.4501139031398869
8	0.4049120276670318	1.2997279197275386
9	0.31214697787754375	1.1272063529737726
>10	2.3022709723564043	18.651584081412807
>50	0.2945521135381719	8.277261942196624
>100	0.20704025371885976	15.659569023597019
>500	0.013873831434768951	3.819187752842817
>1k	0.0032016534080236045	1.3873941382678243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1193	0.2539897467341134	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1127	0.2399383441486553	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1091	0.23227394273840546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.22333214109311397	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1012	0.2154548396436905	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1008	0.21460323948699606	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	993	0.21140973889939196	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	987	0.21013233866435033	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	976	0.20779043823344065	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	959	0.2041711375674893	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	927	0.1973583363139339	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	860	0.18309403368930222	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	824	0.17542963227905234	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	792	0.1686168310254969	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	766	0.1630814300069831	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	700	0.14903002742152505	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	695	0.147965527225657	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	630	0.13412702467937254	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	626	0.13327542452267813	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	625	0.1330625244835045	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	611	0.130081923935074	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	578	0.12305622264234496	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	567	0.12071432221143528	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	558	0.11879822185887283	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	556	0.11837242178052562	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	525	0.1117725205661438	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	525	0.1117725205661438	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	518	0.11028222029192854	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	514	0.10943062013523411	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	513	0.10921772009606048	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	512	0.1090048200568869	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	501	0.10666291962597721	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	471	0.100275918450769	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	470	0.10006301841159537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.129000391736072E-4	0.0	0.0	0.0	0.0
7	2.129000391736072E-4	0.0	0.0	0.0	0.0
8	4.258000783472144E-4	0.0	0.0	0.0	0.0
9	4.258000783472144E-4	0.0	0.0	0.0	0.0
10	4.258000783472144E-4	0.0	0.0	0.0	2.129000391736072E-4
11	4.258000783472144E-4	0.0	0.0	0.0	2.129000391736072E-4
12	4.258000783472144E-4	0.0	0.0	0.0	2.129000391736072E-4
13	4.258000783472144E-4	0.0	0.0	0.0	2.129000391736072E-4
14	4.258000783472144E-4	2.129000391736072E-4	0.0	0.0	2.129000391736072E-4
15	4.258000783472144E-4	2.129000391736072E-4	0.0	2.129000391736072E-4	2.129000391736072E-4
16	4.258000783472144E-4	2.129000391736072E-4	0.0	0.0017032003133888576	2.129000391736072E-4
17	4.258000783472144E-4	2.129000391736072E-4	0.0	0.001916100352562465	2.129000391736072E-4
18	4.258000783472144E-4	2.129000391736072E-4	0.0	0.002341900430909679	2.129000391736072E-4
19	4.258000783472144E-4	2.129000391736072E-4	0.0	0.002980600548430501	2.129000391736072E-4
20	4.258000783472144E-4	2.129000391736072E-4	0.0	0.00383220070512493	2.129000391736072E-4
21	4.258000783472144E-4	2.129000391736072E-4	0.0	0.00681280125355543	2.129000391736072E-4
22	4.258000783472144E-4	2.129000391736072E-4	0.0	0.008516001566944289	2.129000391736072E-4
23	4.258000783472144E-4	2.129000391736072E-4	0.0	0.01064500195868036	2.129000391736072E-4
24	4.258000783472144E-4	2.129000391736072E-4	0.0	0.013412702467937253	2.129000391736072E-4
25	6.387001175208216E-4	2.129000391736072E-4	0.0	0.015541702859673327	2.129000391736072E-4
26	6.387001175208216E-4	2.129000391736072E-4	0.0	0.02129000391736072	2.129000391736072E-4
27	6.387001175208216E-4	2.129000391736072E-4	0.0	0.052799209715054585	2.129000391736072E-4
28	6.387001175208216E-4	2.129000391736072E-4	0.0	0.15456542844003884	2.129000391736072E-4
29	6.387001175208216E-4	2.129000391736072E-4	0.0	0.3108340571934665	2.129000391736072E-4
30	6.387001175208216E-4	2.129000391736072E-4	0.0	0.5026569924888866	2.129000391736072E-4
31	6.387001175208216E-4	2.129000391736072E-4	0.0	1.0398037913238976	2.129000391736072E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	970	0.0	27.845362	1
CTCTGGA	40	5.9327675E-5	27.75	2
GCCTAGT	50	9.076151E-6	25.900002	1
CTATATC	55	1.8995555E-5	23.545454	4
TAGTTCC	55	1.8995555E-5	23.545454	4
GTACTAT	55	1.8995555E-5	23.545454	1
GATGCTA	55	1.8995555E-5	23.545454	14
TCGGGTA	105	4.0017767E-11	22.90476	25
ATGCTAG	60	3.7202517E-5	21.583332	15
AGTTCCG	115	1.3460522E-10	20.913042	32
CGTTAAA	45	0.003822491	20.555555	17
GTTATAG	45	0.003822491	20.555555	11
TAAGTTC	120	2.382876E-10	20.041666	30
CTTGTCG	130	3.274181E-11	19.923077	21
CTAGTTC	65	6.891031E-5	19.923077	3
TCCTTGT	130	3.274181E-11	19.923077	19
TCTCGTC	75	9.246656E-6	19.733334	12
TTCTCGT	75	9.246656E-6	19.733334	11
TCTAATA	85	1.2421024E-6	19.588236	2
ATCTAAT	105	2.2508175E-8	19.38095	1
>>END_MODULE