FastQCFastQC Report
Fri 10 Feb 2017
ERR1631857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856906
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16840.19652097196191878No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15720.18345069354164867No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13790.16092780304957602No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13520.1577769323589752No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13480.1573101367011084No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13340.15567635189857465No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12480.14564024525443864No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11840.13817151472856998No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11750.13712122449836972No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11110.1296524939725011No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10690.12475113956489978No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8620.10059446427029335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.026.0924871
ATACGTC652.6815233E-622.7692313
ATAATAC502.701325E-422.1999993
CCTAGTC603.724861E-521.5833342
TCGTGTT806.95587E-720.812525
ACCGTAT806.95587E-720.81258
TACGTCT450.003825025420.5555554
ATTAGAG1900.020.4473693
GCAGTCG3050.020.0163949
ATACGGC1403.6379788E-1219.82142829
TATACGT759.261261E-619.73333412
CTTAAAC851.2445507E-619.5882363
ACACCGT951.6748709E-719.4736846
TACGGCT1457.2759576E-1219.1379330
CAGTCGG3200.019.07812510
CTTATTG2950.018.81355928
AACGTCA3750.018.74666828
TGTTTAC902.150915E-618.514
CGTTAGA609.2341984E-418.51
TATTAGA2100.018.52
Produced by FastQC (version 0.11.5)