##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631857.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 856906 Sequences flagged as poor quality 0 Sequence length 43 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37879067248917 33.0 31.0 34.0 31.0 34.0 2 32.560641423913474 34.0 31.0 34.0 31.0 34.0 3 32.654766100365734 34.0 31.0 34.0 31.0 34.0 4 36.150538098694604 37.0 35.0 37.0 35.0 37.0 5 36.03206069277144 37.0 35.0 37.0 35.0 37.0 6 36.07779733132922 37.0 35.0 37.0 35.0 37.0 7 36.06659423554042 37.0 35.0 37.0 35.0 37.0 8 36.04562110663247 37.0 35.0 37.0 35.0 37.0 9 37.84952841968664 39.0 38.0 39.0 35.0 39.0 10 37.74392407101829 39.0 37.0 39.0 35.0 39.0 11 37.835873479704894 39.0 38.0 39.0 35.0 39.0 12 37.75566048084621 39.0 37.0 39.0 35.0 39.0 13 37.823080944701054 39.0 38.0 39.0 35.0 39.0 14 39.15677332169456 40.0 39.0 41.0 36.0 41.0 15 39.1486907548786 40.0 39.0 41.0 36.0 41.0 16 39.09637929947976 40.0 38.0 41.0 36.0 41.0 17 39.057667935572866 40.0 38.0 41.0 36.0 41.0 18 39.072927485628526 40.0 38.0 41.0 36.0 41.0 19 39.08821621041281 40.0 39.0 41.0 36.0 41.0 20 39.08180010409543 40.0 39.0 41.0 36.0 41.0 21 39.04621627109625 40.0 39.0 41.0 36.0 41.0 22 39.01807316088346 40.0 39.0 41.0 36.0 41.0 23 38.95810975766303 40.0 38.0 41.0 36.0 41.0 24 38.97308923032398 40.0 38.0 41.0 35.0 41.0 25 38.89839375614128 40.0 38.0 41.0 35.0 41.0 26 38.82470305961214 40.0 38.0 41.0 35.0 41.0 27 38.72583223830852 40.0 38.0 41.0 35.0 41.0 28 38.7086693289579 40.0 38.0 41.0 35.0 41.0 29 38.67532028017075 40.0 38.0 41.0 35.0 41.0 30 38.628053718844306 40.0 38.0 41.0 35.0 41.0 31 38.55156458234625 40.0 38.0 41.0 35.0 41.0 32 38.509233218112605 40.0 38.0 41.0 34.0 41.0 33 38.44468938249936 40.0 38.0 41.0 34.0 41.0 34 38.41402674272324 40.0 38.0 41.0 34.0 41.0 35 38.340730488525 40.0 38.0 41.0 34.0 41.0 36 38.30608374780898 40.0 38.0 41.0 34.0 41.0 37 38.25462069351831 40.0 38.0 41.0 34.0 41.0 38 38.21113401003144 40.0 38.0 41.0 34.0 41.0 39 38.14063502881296 40.0 37.0 41.0 34.0 41.0 40 37.819473781254885 40.0 37.0 41.0 33.0 41.0 41 37.902701113074244 40.0 37.0 41.0 33.0 41.0 42 37.889342588335246 40.0 37.0 41.0 33.0 41.0 43 37.387350537865295 39.0 36.0 41.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 1.0 13 0.0 14 0.0 15 1.0 16 1.0 17 2.0 18 3.0 19 24.0 20 32.0 21 69.0 22 159.0 23 352.0 24 564.0 25 927.0 26 1503.0 27 2393.0 28 3540.0 29 5218.0 30 7266.0 31 9771.0 32 13309.0 33 17947.0 34 26453.0 35 38791.0 36 59646.0 37 105791.0 38 240602.0 39 322539.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.99771503525474 16.891817772311082 12.358531740937746 27.75193545149643 2 20.767505420664577 19.228830233421167 32.58898875722658 27.414675588687675 3 20.891556366742677 20.21960401724343 28.953584173760017 29.935255442253876 4 15.518388247952517 15.704406317612435 34.01539958875303 34.76180584568203 5 16.844904808695468 32.951922381218004 33.73788957015122 16.465283239935303 6 35.048651777441165 33.879678751228255 15.080067125215601 15.991602346114977 7 30.880283251605196 27.642588568641134 20.319148191283524 21.157979988470146 8 27.81040160764425 30.838855136969517 19.694925697801157 21.65581755758508 9 28.07285746627985 12.844115924033675 17.803236294295992 41.279790315390485 10 19.056699334582788 23.658137532004677 30.346035621176654 26.939127512235885 11 39.45671987359173 19.304101033252188 19.04713002359652 22.192049069559555 12 23.301388950479982 23.685911873647754 26.61657171264993 26.396127463222335 13 34.20946988351115 17.872322051660277 20.78092579582825 27.137282269000334 14 24.045227831290713 19.415198399824483 23.118288353681734 33.421285415203066 15 29.08487045253505 24.330439978247323 20.20968460951376 26.375004959703862 16 26.254104884316366 23.93658114192222 22.239312129918567 27.57000184384285 17 26.81099210415145 23.710535344600224 22.00042945200524 27.478043099243095 18 26.707946962677354 22.096939454269197 23.374909266594003 27.820204316459446 19 27.87843707477833 22.458239293458092 23.31562621804492 26.347697413718656 20 29.138551953189733 21.327776909019192 22.88208975080114 26.651581386989935 21 27.25363108672363 22.671681608017682 22.162407545285014 27.912279759973675 22 28.262726600117166 23.063906659540255 21.46734881072136 27.20601792962122 23 27.731979937122624 22.49966740809377 22.511103901711508 27.2572487530721 24 27.7643055364299 22.25903424646344 22.32228505810439 27.65437515900227 25 27.676197856007544 22.182479758573287 23.027029802568777 27.114292582850396 26 27.698020553012814 22.788730619227778 22.525807964934312 26.987440862825096 27 27.002261624962365 22.095305669466665 23.411552725736545 27.490879979834425 28 26.430086847332145 22.751386966598435 22.525924663848777 28.29260152222064 29 27.41946024418081 23.226351548477897 22.635504944533004 26.71868326280829 30 26.901200365034207 22.063680263646187 23.597103999738593 27.438015371581017 31 27.54106051305511 22.645541051177144 22.785696447451645 27.027701988316107 32 25.881135153680802 22.483096162239498 22.85023094715173 28.785537736927967 33 26.017206087948967 22.088887229170993 24.488333609520765 27.405573073359275 34 26.407914053583475 22.055978135291383 23.883949931497735 27.652157879627403 35 25.84437499562379 23.547973756748114 24.190401280887286 26.41724996674081 36 26.34933119852119 22.46862549684563 24.35634713725893 26.825696167374254 37 26.26822545296684 22.369431419548935 24.420764938044545 26.94157818943968 38 25.792327279771644 21.71591749853543 25.25189460687637 27.239860614816564 39 25.23298938273276 21.148644075312813 25.978578747260496 27.63978779469393 40 25.197396213820415 21.953166391646224 26.55997273913358 26.289464655399776 41 23.904722338272812 21.065554448212524 27.478393195986488 27.551330017528176 42 22.517172245263776 21.78441976132738 28.145210793249202 27.553197200159644 43 22.25611677360177 21.704364306003228 28.136341675749733 27.90317724464527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 4.5 2 7.0 3 10.5 4 14.0 5 14.0 6 28.5 7 43.0 8 45.5 9 48.0 10 75.0 11 102.0 12 102.0 13 218.0 14 334.0 15 629.0 16 924.0 17 914.5 18 905.0 19 905.0 20 1082.0 21 1259.0 22 1129.5 23 1000.0 24 1291.5 25 1583.0 26 1583.0 27 1975.5 28 2368.0 29 3231.5 30 4095.0 31 5600.5 32 7106.0 33 7106.0 34 9628.5 35 12151.0 36 14497.0 37 16843.0 38 20895.5 39 24948.0 40 24948.0 41 28851.5 42 32755.0 43 38653.5 44 44552.0 45 54073.5 46 63595.0 47 63595.0 48 68574.5 49 73554.0 50 82027.0 51 90500.0 52 90686.5 53 90873.0 54 90873.0 55 84992.5 56 79112.0 57 75269.0 58 71426.0 59 67342.5 60 63259.0 61 63259.0 62 57681.5 63 52104.0 64 44690.5 65 37277.0 66 32706.0 67 28135.0 68 28135.0 69 23996.5 70 19858.0 71 16709.0 72 13560.0 73 11186.5 74 8813.0 75 8813.0 76 7151.5 77 5490.0 78 4465.0 79 3440.0 80 2854.0 81 2268.0 82 2268.0 83 1774.0 84 1280.0 85 1004.5 86 729.0 87 540.0 88 351.0 89 351.0 90 261.5 91 172.0 92 113.5 93 55.0 94 34.5 95 14.0 96 14.0 97 8.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 856906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.45469039996665 #Duplication Level Percentage of deduplicated Percentage of total 1 86.48590430994346 47.09563143159113 2 7.109861797341802 7.743306459215966 3 2.2205474473120703 3.6275767128544523 4 1.0619524400153721 2.31313165362105 5 0.6226603401445904 1.69533880234558 6 0.39570742011003174 1.2928875030636788 7 0.267653122990079 1.0202477562906263 8 0.2099760654061495 0.9147345306476018 9 0.15882664970285867 0.7783970433149813 >10 1.2109485275006218 13.14996119626187 >50 0.14038076428285656 5.3696145256699825 >100 0.10652438607222654 11.081081142145862 >500 0.00668472867891243 2.2206078909084317 >1k 0.002372000498968927 1.6974833520687582 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA 1684 0.19652097196191878 No Hit ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA 1572 0.18345069354164867 No Hit CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC 1379 0.16092780304957602 No Hit CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA 1352 0.1577769323589752 No Hit GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA 1348 0.1573101367011084 No Hit GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA 1334 0.15567635189857465 No Hit TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT 1248 0.14564024525443864 No Hit GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG 1184 0.13817151472856998 No Hit GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC 1175 0.13712122449836972 No Hit CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA 1111 0.1296524939725011 No Hit GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT 1069 0.12475113956489978 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 862 0.10059446427029335 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 1.1669891446669764E-4 0.0 15 0.0 0.0 0.0 1.1669891446669764E-4 0.0 16 0.0 0.0 0.0 5.834945723334882E-4 0.0 17 0.0 0.0 0.0 9.335913157335811E-4 0.0 18 0.0 0.0 0.0 0.0011669891446669763 0.0 19 0.0 0.0 0.0 0.0014003869736003715 0.0 20 0.0 0.0 0.0 0.0023339782893339526 0.0 21 1.1669891446669764E-4 0.0 0.0 0.002917472861667441 0.0 22 2.3339782893339527E-4 0.0 0.0 0.00443455874973451 0.0 23 2.3339782893339527E-4 0.0 0.0 0.0049013544076013 0.0 24 2.3339782893339527E-4 0.0 0.0 0.00641844029566837 0.0 25 2.3339782893339527E-4 0.0 0.0 0.007585429440335346 0.0 26 2.3339782893339527E-4 0.0 0.0 0.00933591315733581 0.0 27 2.3339782893339527E-4 0.0 0.0 0.023806578551206315 0.0 28 2.3339782893339527E-4 0.0 0.0 0.0886911749946902 0.0 29 2.3339782893339527E-4 0.0 0.0 0.18590137074544932 0.0 30 2.3339782893339527E-4 0.0 0.0 0.32103871369788517 0.0 31 2.3339782893339527E-4 0.0 0.0 0.6994932933133856 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 865 0.0 26.092487 1 ATACGTC 65 2.6815233E-6 22.76923 13 ATAATAC 50 2.701325E-4 22.199999 3 CCTAGTC 60 3.724861E-5 21.583334 2 TCGTGTT 80 6.95587E-7 20.8125 25 ACCGTAT 80 6.95587E-7 20.8125 8 TACGTCT 45 0.0038250254 20.555555 4 ATTAGAG 190 0.0 20.447369 3 GCAGTCG 305 0.0 20.016394 9 ATACGGC 140 3.6379788E-12 19.821428 29 TATACGT 75 9.261261E-6 19.733334 12 CTTAAAC 85 1.2445507E-6 19.588236 3 ACACCGT 95 1.6748709E-7 19.473684 6 TACGGCT 145 7.2759576E-12 19.13793 30 CAGTCGG 320 0.0 19.078125 10 CTTATTG 295 0.0 18.813559 28 AACGTCA 375 0.0 18.746668 28 TGTTTAC 90 2.150915E-6 18.5 14 CGTTAGA 60 9.2341984E-4 18.5 1 TATTAGA 210 0.0 18.5 2 >>END_MODULE