FastQCFastQC Report
Fri 10 Feb 2017
ERR1631856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101846
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC2880.2827798833532981No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT2240.21993990927478743No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2000.1963749189953459No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1400.13746244329674212No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1390.13648056870176542No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1280.12567994815702138No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1270.12469807356204465No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1240.12175244977711445No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT1210.11880682599218427No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1170.11487932761227737No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC1160.11389745301730063No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1160.11389745301730063No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT1150.1129155784223239No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1130.11095182923237044No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT1120.10996995463739372No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC1060.10407870706753333No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG1040.10211495787757988No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1020.10015120868762642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGCT658.54925E-1131.3076928
GAACCAG303.5766815E-430.83333435
GCCATCA751.4551915E-1129.627
CATCAGA751.4551915E-1129.616
TAATACT250.005473133629.5999984
ACAAGAA250.005473133629.59999812
GGTATCA900.028.7777771
GGCCATC803.092282E-1127.75000226
ATAGTGA405.887916E-527.75000230
TAGTGAA405.887916E-527.75000231
AGAGGCC803.092282E-1127.75000223
CGATAGT405.887916E-527.75000228
GCTTTAT405.887916E-527.7500021
GAGGCCA803.092282E-1127.75000224
GGTTTCT358.8137516E-426.42857215
TCAAGCA706.4428605E-926.42857231
ATCAGAA856.730261E-1126.11764717
AGAAGAG856.730261E-1126.11764720
AGGCCAT856.730261E-1126.11764725
CCATCAA856.730261E-1126.11764728
Produced by FastQC (version 0.11.5)