##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631856.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101846
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81318853955973	31.0	31.0	34.0	30.0	34.0
2	32.01387388802702	33.0	31.0	34.0	30.0	34.0
3	32.1055122439762	33.0	31.0	34.0	30.0	34.0
4	35.78637354437091	37.0	35.0	37.0	35.0	37.0
5	35.477024134477546	37.0	35.0	37.0	33.0	37.0
6	35.48573336213499	37.0	35.0	37.0	33.0	37.0
7	35.49559138306856	37.0	35.0	37.0	33.0	37.0
8	35.451839051116394	37.0	35.0	37.0	33.0	37.0
9	37.21777978516584	39.0	37.0	39.0	34.0	39.0
10	37.02623568917778	39.0	37.0	39.0	33.0	39.0
11	37.10589517506824	39.0	37.0	39.0	33.0	39.0
12	37.03948117746401	39.0	37.0	39.0	33.0	39.0
13	37.11047071068083	39.0	37.0	39.0	33.0	39.0
14	38.25231231467117	40.0	38.0	41.0	34.0	41.0
15	38.218093985036234	40.0	38.0	41.0	34.0	41.0
16	38.15825854721835	40.0	38.0	41.0	33.0	41.0
17	38.109891404669796	40.0	38.0	41.0	33.0	41.0
18	38.09608624786442	40.0	38.0	41.0	33.0	41.0
19	38.11321014080082	40.0	38.0	41.0	34.0	41.0
20	38.070272764762485	40.0	37.0	41.0	33.0	41.0
21	38.05108693517664	40.0	37.0	41.0	33.0	41.0
22	37.97932172102979	40.0	37.0	41.0	33.0	41.0
23	37.93160261571392	40.0	37.0	41.0	33.0	41.0
24	37.93590322643992	40.0	37.0	41.0	33.0	41.0
25	37.84556094495611	39.0	37.0	41.0	33.0	41.0
26	37.7151483612513	39.0	37.0	40.0	33.0	41.0
27	37.62317616793983	39.0	37.0	40.0	33.0	41.0
28	37.59646917895646	39.0	37.0	40.0	33.0	41.0
29	37.52978025646564	39.0	37.0	40.0	32.0	41.0
30	37.48404453783163	39.0	37.0	40.0	32.0	41.0
31	37.411120711662704	39.0	36.0	40.0	32.0	41.0
32	37.366779254953556	39.0	36.0	40.0	32.0	41.0
33	37.29870588928382	39.0	36.0	40.0	32.0	41.0
34	37.225045657168664	39.0	36.0	40.0	31.0	41.0
35	37.085275808573726	39.0	36.0	40.0	31.0	41.0
36	37.079688942128314	39.0	36.0	40.0	31.0	41.0
37	37.003475836066215	39.0	36.0	40.0	31.0	41.0
38	37.00468354181804	39.0	36.0	40.0	31.0	41.0
39	36.94338511085364	39.0	35.0	40.0	31.0	41.0
40	36.57506431278597	39.0	35.0	40.0	30.0	41.0
41	36.66135145219253	39.0	35.0	40.0	31.0	41.0
42	36.632759263986806	38.0	35.0	40.0	31.0	41.0
43	36.05741020756829	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	11.0
21	23.0
22	58.0
23	90.0
24	133.0
25	213.0
26	346.0
27	516.0
28	795.0
29	1093.0
30	1405.0
31	2038.0
32	2727.0
33	3719.0
34	5090.0
35	7683.0
36	11934.0
37	19594.0
38	29016.0
39	15355.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.59273805549555	16.85191367358561	12.455079237279815	28.100269033639023
2	21.34693556938908	18.633034188873395	32.84075957818667	27.179270663550852
3	21.903658464740882	19.833866818529938	28.184710248807022	30.077764467922158
4	16.50923943993873	15.266186202698192	32.7592639868036	35.46531037055947
5	17.300630363489976	32.91047267443002	32.769082732753375	17.01981422932663
6	36.1771694519176	32.75631836301868	14.543526500795318	16.522985684268406
7	31.51130137658818	26.903363902362393	19.656147516839148	21.92918720421028
8	27.935314101682934	31.122479036977396	19.167173968540737	21.775032892798933
9	28.396795161321997	12.653417905465114	17.00115861202207	41.94862832119082
10	20.047915480234867	23.237044164719283	29.336449148714728	27.378591206331127
11	39.42226498831569	19.47548259136343	18.46120613475247	22.641046285568407
12	24.52820925711368	23.178131689020677	25.517938848850225	26.775720205015414
13	33.47897806492155	17.738546432849596	20.88447263515504	27.898002867073817
14	23.932211377962805	19.216267698289574	22.918916795946824	33.9326041278008
15	29.606464662333327	23.39119847613063	20.124501698643048	26.877835162892993
16	26.498831569231978	23.500186556173045	21.3459536947941	28.655028179800873
17	27.130176933802012	23.671032735698997	21.5570567327141	27.64173359778489
18	26.191504821004262	21.44610490348173	23.464839070753886	28.89755120476013
19	28.350647055358092	21.983190306934	22.389686389254365	27.276476248453545
20	28.521493234884044	20.91098324921941	23.46287532156393	27.10464819433262
21	28.877913712860593	21.53545549162461	21.39406554994796	28.192565245566836
22	29.035013648056868	23.088781100877796	20.9198201205742	26.956385130491135
23	28.642263810066176	22.57722443689492	21.444141154291774	27.336370598747127
24	28.98395616911808	21.79663413388842	21.41468491644247	27.804724780551027
25	28.30548082398916	21.66113543978163	22.905170551617147	27.12821318461206
26	28.525420733263946	22.35924827681009	22.44467136657306	26.670659623352904
27	28.750270015513617	21.44610490348173	22.34550203248041	27.458123048524243
28	26.250417296702867	22.110834004280974	22.737269995876126	28.901478703140032
29	27.916658484378377	22.326846415175854	22.672466272607664	27.084028827838107
30	27.20087190464034	21.33122557586945	23.855625159554624	27.61227735993559
31	27.481688038803686	22.047012155607487	22.904188677022173	27.56711112856666
32	25.71431376784557	22.101015258331206	23.206606052275003	28.978064921548217
33	26.96031262887104	22.181528975119296	23.51098717671779	27.347171219291873
34	26.947548259136344	22.156000235649902	23.650413369204486	27.246038136009272
35	26.761973960685744	22.819747461854174	24.12073130019834	26.297547277261746
36	27.037880721874203	21.56294798028396	23.465820945348863	27.93335035249298
37	25.771262494354218	21.729866661430002	24.544901125228286	27.953969718987494
38	26.63531213793374	20.58401900909216	25.339237672564458	27.441431180409637
39	24.900339728609865	20.52707028258351	26.585236533589928	27.987353455216702
40	25.154645248708835	20.671405848045087	27.3373524733421	26.836596429903974
41	23.393162225320584	19.843685564479703	28.37813954401744	28.385012666182273
42	21.934096577185162	21.026844451426662	28.68448441765018	28.354574553737997
43	21.903658464740882	20.36997034738723	29.1940773324431	28.532293855428787
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.0
4	3.0
5	3.0
6	7.0
7	11.0
8	10.0
9	9.0
10	12.5
11	16.0
12	16.0
13	34.5
14	53.0
15	101.5
16	150.0
17	135.0
18	120.0
19	120.0
20	148.5
21	177.0
22	139.0
23	101.0
24	127.0
25	153.0
26	153.0
27	172.5
28	192.0
29	279.5
30	367.0
31	504.5
32	642.0
33	642.0
34	950.5
35	1259.0
36	1496.5
37	1734.0
38	2249.0
39	2764.0
40	2764.0
41	3155.0
42	3546.0
43	4329.0
44	5112.0
45	5872.0
46	6632.0
47	6632.0
48	7356.0
49	8080.0
50	9258.0
51	10436.0
52	10789.5
53	11143.0
54	11143.0
55	10747.5
56	10352.0
57	9573.5
58	8795.0
59	8056.0
60	7317.0
61	7317.0
62	6984.0
63	6651.0
64	5360.5
65	4070.0
66	3598.5
67	3127.0
68	3127.0
69	2659.0
70	2191.0
71	2073.5
72	1956.0
73	2105.0
74	2254.0
75	2254.0
76	1869.5
77	1485.0
78	1022.5
79	560.0
80	366.0
81	172.0
82	172.0
83	135.5
84	99.0
85	76.0
86	53.0
87	43.5
88	34.0
89	34.0
90	26.5
91	19.0
92	14.0
93	9.0
94	4.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	101846.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.206880977161596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.04072848859958	46.948333758812325
2	6.4436381745100135	7.114663315201382
3	2.4188098032938496	4.006048347505057
4	1.3783658805534806	3.0438112444278618
5	0.8874897734144346	2.4497771144669405
6	0.5904741578629104	1.955894193193645
7	0.4677551310781489	1.807631129352159
8	0.3877209831750436	1.7123892936394165
9	0.23832390708924694	1.1841407615419357
>10	1.947497598975563	21.264458103411034
>50	0.1654039056664177	5.966851913673586
>100	0.03379219578131114	2.5460008247746595
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	288	0.2827798833532981	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	224	0.21993990927478743	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	200	0.1963749189953459	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	140	0.13746244329674212	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	139	0.13648056870176542	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	128	0.12567994815702138	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	127	0.12469807356204465	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	124	0.12175244977711445	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	121	0.11880682599218427	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	117	0.11487932761227737	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	116	0.11389745301730063	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	116	0.11389745301730063	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	115	0.1129155784223239	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	113	0.11095182923237044	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	112	0.10996995463739372	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	106	0.10407870706753333	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	104	0.10211495787757988	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	102	0.10015120868762642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	9.818745949767295E-4	0.0	0.0	0.0	0.0
9	9.818745949767295E-4	0.0	0.0	0.0	0.0
10	9.818745949767295E-4	0.0	0.0	0.0	0.0
11	9.818745949767295E-4	0.0	0.0	0.0	0.0
12	9.818745949767295E-4	0.0	0.0	0.0	0.0
13	9.818745949767295E-4	0.0	0.0	0.0	0.0
14	9.818745949767295E-4	0.0	0.0	0.0	0.0
15	0.001963749189953459	0.0	0.0	0.0	0.0
16	0.001963749189953459	0.0	0.0	0.0	0.0
17	0.001963749189953459	0.0	0.0	0.0	0.0
18	0.001963749189953459	0.0	0.0	9.818745949767295E-4	0.0
19	0.001963749189953459	0.0	0.0	9.818745949767295E-4	0.0
20	0.001963749189953459	0.0	0.0	9.818745949767295E-4	0.0
21	0.001963749189953459	0.0	0.0	0.001963749189953459	0.0
22	0.001963749189953459	0.0	0.0	0.003927498379906918	0.0
23	0.001963749189953459	0.0	0.0	0.007854996759813836	0.0
24	0.001963749189953459	0.0	0.0	0.010800620544744025	0.0
25	0.001963749189953459	0.0	0.0	0.011782495139720755	0.0
26	0.001963749189953459	0.0	0.0	0.015709993519627673	0.0
27	0.001963749189953459	0.0	0.0	0.039274983799069185	0.0
28	0.001963749189953459	0.0	0.0	0.13451681951181196	0.0
29	0.001963749189953459	0.0	0.0	0.26510614064371696	0.0
30	0.001963749189953459	0.0	0.0	0.4477348153093887	0.0
31	0.001963749189953459	0.0	0.0	0.969110225242032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGGCT	65	8.54925E-11	31.307692	8
GAACCAG	30	3.5766815E-4	30.833334	35
GCCATCA	75	1.4551915E-11	29.6	27
CATCAGA	75	1.4551915E-11	29.6	16
TAATACT	25	0.0054731336	29.599998	4
ACAAGAA	25	0.0054731336	29.599998	12
GGTATCA	90	0.0	28.777777	1
GGCCATC	80	3.092282E-11	27.750002	26
ATAGTGA	40	5.887916E-5	27.750002	30
TAGTGAA	40	5.887916E-5	27.750002	31
AGAGGCC	80	3.092282E-11	27.750002	23
CGATAGT	40	5.887916E-5	27.750002	28
GCTTTAT	40	5.887916E-5	27.750002	1
GAGGCCA	80	3.092282E-11	27.750002	24
GGTTTCT	35	8.8137516E-4	26.428572	15
TCAAGCA	70	6.4428605E-9	26.428572	31
ATCAGAA	85	6.730261E-11	26.117647	17
AGAAGAG	85	6.730261E-11	26.117647	20
AGGCCAT	85	6.730261E-11	26.117647	25
CCATCAA	85	6.730261E-11	26.117647	28
>>END_MODULE