FastQCFastQC Report
Fri 10 Feb 2017
ERR1631855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58788
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2940.5001020616452337No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2700.4592774035517453No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2580.43886507450500106No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2300.39123630672926446No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2190.3725250051030823No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1940.3299993195890318No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1890.3214941824862217No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1860.31639110022453565No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1850.3146900728039736No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1780.30278288086003946No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1630.2772674695516092No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1500.2551541130843029No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1410.23984486629924476No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1230.20922637272912842No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1230.20922637272912842No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1190.2024222630468803No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1170.1990202082057563No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1160.19731918078519425No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1140.1939171259440702No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1120.1905150711029462No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1070.18200993400013607No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1040.17690685173845003No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1040.17690685173845003No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1030.175205824317888No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC990.16840171463563994No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG990.16840171463563994No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC980.16670068721507791No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT970.1649996597945159No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT960.16329863237395387No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC940.15989657753282982No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC920.1564945226917058No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA920.1564945226917058No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT920.1564945226917058No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG910.15479349527114378No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC910.15479349527114378No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT910.15479349527114378No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG890.15139144043001973No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC890.15139144043001973No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA860.1462883581683337No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA860.1462883581683337No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG840.14288630332720964No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA830.14118527590664762No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG800.13608219364496157No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC790.13438116622439955No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT780.13268013880383753No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC780.13268013880383753No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA780.13268013880383753No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC760.12927808396271348No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC760.12927808396271348No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC750.12757705654215146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT740.12587602912158943No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG720.1224739742804654No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC710.12077294685990338No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA710.12077294685990338No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA700.11907191943934137No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC690.11737089201877934No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA680.11566986459821732No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG680.11566986459821732No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC670.1139688371776553No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT670.1139688371776553No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG650.11056678233653126No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG640.10886575491596924No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG640.10886575491596924No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC630.10716472749540723No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC630.10716472749540723No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA620.1054637000748452No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC600.10206164523372117No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT590.10036061781315915No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG590.10036061781315915No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC590.10036061781315915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTAT250.005454519829.636
ATCTGTC358.7692024E-426.4285737
GGTATCA1200.026.2083341
TTAGCCC400.001909577523.12529
TGAGCAA400.001909577523.12536
CTCTTAT1750.022.237
GCGGTAA1250.022.19999923
AGCCGCG1301.8189894E-1221.34615319
CAGCAGC1351.8189894E-1220.55555515
CCAGCAG1351.8189894E-1220.55555514
ATGAGCA450.003783405820.55555535
CGCGGTA1450.020.41379422
GCCCTGT555.063714E-420.18181832
CGTGCCA1403.6379788E-1219.82142810
TCCGTGC1403.6379788E-1219.8214288
GTGCCAG1403.6379788E-1219.82142811
GTATCAA1600.019.656252
CCGTGCC1457.2759576E-1219.137939
GCCAGCA1457.2759576E-1219.1379313
GCAGCCG1457.2759576E-1219.1379317
Produced by FastQC (version 0.11.5)