FastQCFastQC Report
Fri 10 Feb 2017
ERR1631854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143941
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2930.2035556234846222No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2570.1785453762305389No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2410.16742971078427968No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2380.16534552351310608No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2350.16326133624193245No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2330.16187187806115005No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2210.15353512897645563No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2050.1424194635301964No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2040.14172473443980518No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1840.12783015263198116No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1790.12435650718002515No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1690.11740921627611313No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1600.11115665446259232No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1520.1055988217394627No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1480.1028199053778979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG303.5839877E-430.8333341
GGTATCA1700.030.4705891
ATTGTAG250.005480577229.5999983
CCGCTTA250.005480577229.59999825
CTAAACC250.005480577229.5999984
GCTTATT250.005480577229.59999827
ACGTGTG250.005480577229.59999811
TCGTAAG250.005480577229.59999827
GTAAGGG250.005480577229.59999829
TTATTGA604.267713E-827.75000229
TGATTCC556.197988E-726.9090917
ATTGATA556.197988E-726.9090931
AAGAGTC358.831587E-426.428577
CATTGTA358.831587E-426.428572
GTGATTC706.490154E-926.4285716
CTTATTG659.273026E-825.61538528
GATTCCT601.3238714E-624.66666818
CTTATAC1600.024.28125237
GATATGC551.887707E-523.54545434
AGCGTAC400.001923032823.1250021
Produced by FastQC (version 0.11.5)