##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631854.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	143941
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51587803336089	34.0	31.0	34.0	31.0	34.0
2	32.6970286436804	34.0	31.0	34.0	31.0	34.0
3	32.80100874663925	34.0	31.0	34.0	31.0	34.0
4	36.23479759067951	37.0	37.0	37.0	35.0	37.0
5	36.13844561313316	37.0	35.0	37.0	35.0	37.0
6	36.18702107113331	37.0	36.0	37.0	35.0	37.0
7	36.180407250192786	37.0	36.0	37.0	35.0	37.0
8	36.15874559715439	37.0	36.0	37.0	35.0	37.0
9	37.98678625270076	39.0	38.0	39.0	35.0	39.0
10	37.893914867897266	39.0	38.0	39.0	35.0	39.0
11	37.977997929707314	39.0	38.0	39.0	35.0	39.0
12	37.89961164643847	39.0	38.0	39.0	35.0	39.0
13	37.96349893359085	39.0	38.0	39.0	35.0	39.0
14	39.32450795812173	40.0	39.0	41.0	36.0	41.0
15	39.33518594424104	40.0	39.0	41.0	36.0	41.0
16	39.271062449197935	40.0	39.0	41.0	36.0	41.0
17	39.24119604560202	40.0	39.0	41.0	36.0	41.0
18	39.27344536997798	40.0	39.0	41.0	36.0	41.0
19	39.284943136423955	40.0	39.0	41.0	36.0	41.0
20	39.27887120417393	40.0	39.0	41.0	36.0	41.0
21	39.23500600940663	40.0	39.0	41.0	36.0	41.0
22	39.21348330218631	40.0	39.0	41.0	36.0	41.0
23	39.136875525388874	40.0	39.0	41.0	36.0	41.0
24	39.16061441840754	40.0	39.0	41.0	36.0	41.0
25	39.0944762090023	40.0	39.0	41.0	36.0	41.0
26	39.03784189355361	40.0	39.0	41.0	35.0	41.0
27	38.917021557443675	40.0	38.0	41.0	35.0	41.0
28	38.907809449705084	40.0	38.0	41.0	35.0	41.0
29	38.88362593006857	40.0	38.0	41.0	35.0	41.0
30	38.84287312162622	40.0	38.0	41.0	35.0	41.0
31	38.761631501795875	40.0	38.0	41.0	35.0	41.0
32	38.70924198108948	40.0	38.0	41.0	35.0	41.0
33	38.64811276842595	40.0	38.0	41.0	35.0	41.0
34	38.61343883952453	40.0	38.0	41.0	35.0	41.0
35	38.53764389576285	40.0	38.0	41.0	35.0	41.0
36	38.50141377369894	40.0	38.0	41.0	35.0	41.0
37	38.46129316872885	40.0	38.0	41.0	34.0	41.0
38	38.414607373854565	40.0	38.0	41.0	34.0	41.0
39	38.35693791206119	40.0	38.0	41.0	34.0	41.0
40	38.017194544987184	40.0	37.0	41.0	33.0	41.0
41	38.10834300164651	40.0	37.0	41.0	34.0	41.0
42	38.089293529987984	40.0	37.0	41.0	34.0	41.0
43	37.60115602920641	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	13.0
22	16.0
23	48.0
24	77.0
25	147.0
26	218.0
27	362.0
28	519.0
29	778.0
30	1097.0
31	1427.0
32	1951.0
33	2745.0
34	3857.0
35	5869.0
36	9204.0
37	15974.0
38	36660.0
39	62972.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.89674241529516	16.267081651509994	12.576680723351929	28.25949520984292
2	21.248983958705303	19.01612466218798	31.962401261628028	27.77249011747869
3	21.485886578528703	19.13214442028331	28.403304131553902	30.978664869634088
4	16.0454630716752	15.420206890323119	32.88013839003481	35.654191647966876
5	17.81910643944394	31.970043281622328	32.77592902647613	17.434921252457606
6	35.455499128114994	32.7620344446683	15.02282185061935	16.759644576597353
7	31.438575527473063	26.847110969077608	19.742116561646785	21.972196941802544
8	27.992024510042306	30.213768141113373	19.576076309043287	22.21813103980103
9	28.704121827693292	12.780236346836551	17.360585239785745	41.15505658568441
10	19.799779076149253	23.10321590095942	29.53988092343391	27.557124099457415
11	39.88161816299734	18.884820864104043	18.549961442535484	22.683599530363136
12	23.510327147928663	23.080984570066903	26.476125634808707	26.932562647195724
13	35.027546008434015	17.20010281990538	20.332636288479307	27.439714883181303
14	24.457242898131874	19.558708081783507	22.793366726644944	33.19068229343967
15	30.03105439034049	23.678451587803337	19.744200748917958	26.546293272938215
16	26.314253756747558	23.418622907997026	22.052785516287923	28.214337818967493
17	27.220180490617686	23.44293842616072	21.483802391257527	27.85307869196407
18	27.277148276029763	21.203826567829875	23.008038015575828	28.510987140564538
19	28.506124036931794	21.931902654559853	23.25397211357431	26.308001194934032
20	29.8955822177142	20.7439159099909	22.48838065596321	26.872121216331692
21	27.61061823941754	22.146573943490736	21.933292112740638	28.30951570435109
22	28.425535462446415	22.619684454047146	21.261489082332343	27.693291001174092
23	28.34564161705143	21.71653663653858	22.198678625270077	27.739143121139914
24	28.17334880263441	21.48866549489027	22.53701169229059	27.800974010184724
25	28.157370033555416	21.6991684092788	22.728756921238563	27.414704635927222
26	28.06566579362378	22.371666168777484	22.320256216088534	27.242411821510203
27	27.289653399656803	21.87215595278621	23.58883153514287	27.249359112414112
28	26.773469685496142	22.39111858330844	22.168805274383256	28.666606456812165
29	27.659944004835314	22.916333775644187	22.323729861540492	27.099992357980007
30	27.226433052431204	21.556054216658215	23.255361571755095	27.962151159155486
31	27.96284588824588	21.8492298928033	22.648863075843575	27.539061143107247
32	26.346906023995942	21.98261787815841	22.693325737628612	28.977150360217035
33	26.393452873052155	21.608158898437555	24.4155591527084	27.58282907580189
34	26.884626339958732	21.492833869432616	23.738198289576978	27.884341501031674
35	26.313559027657163	22.769051208481255	24.170319783800306	26.747069980061276
36	26.576861352915433	21.65262156022259	24.329412745499894	27.441104341362088
37	26.63869224196025	21.38723504769315	24.50656866354965	27.467504046796954
38	26.197539269561837	20.721684579098383	25.490305055543587	27.590471095796193
39	25.490999784633985	20.281226335790357	26.195455082290657	28.032318797285
40	24.981763361377233	20.99332365344134	27.018014325313843	27.006898659867584
41	23.81461848952001	20.163122390423855	28.031624068194606	27.990635051861528
42	22.414044643291348	20.644569650064955	29.07302297469102	27.868362731952672
43	21.713062991086627	21.42752933493584	28.343557429780258	28.515850244197278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	4.5
4	9.0
5	9.0
6	9.5
7	10.0
8	10.0
9	10.0
10	19.5
11	29.0
12	29.0
13	55.5
14	82.0
15	172.0
16	262.0
17	245.5
18	229.0
19	229.0
20	265.0
21	301.0
22	234.5
23	168.0
24	199.5
25	231.0
26	231.0
27	280.0
28	329.0
29	424.5
30	520.0
31	699.0
32	878.0
33	878.0
34	1168.5
35	1459.0
36	1763.0
37	2067.0
38	2617.5
39	3168.0
40	3168.0
41	3794.5
42	4421.0
43	5244.0
44	6067.0
45	8043.0
46	10019.0
47	10019.0
48	11188.0
49	12357.0
50	13837.5
51	15318.0
52	15620.5
53	15923.0
54	15923.0
55	14946.5
56	13970.0
57	13379.5
58	12789.0
59	12230.0
60	11671.0
61	11671.0
62	10623.0
63	9575.0
64	8160.5
65	6746.0
66	5951.5
67	5157.0
68	5157.0
69	4419.0
70	3681.0
71	3136.0
72	2591.0
73	2036.5
74	1482.0
75	1482.0
76	1241.0
77	1000.0
78	782.5
79	565.0
80	478.5
81	392.0
82	392.0
83	314.0
84	236.0
85	172.0
86	108.0
87	93.0
88	78.0
89	78.0
90	53.5
91	29.0
92	20.0
93	11.0
94	6.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	143941.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.50016326133624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.37454686014563	58.76921794346295
2	6.060321141651257	8.060246906718724
3	1.9723989511183544	3.934945567975768
4	0.9036679516511529	2.4037626527535587
5	0.5442901766592493	1.8097692804690813
6	0.38027183167747935	1.5172883334143852
7	0.25908630290113976	1.2060497009191267
8	0.2047617555186427	1.0893352137334047
9	0.14730309963330931	0.8816112157064352
>10	1.0457475371130682	13.169979366546015
>50	0.0699950898966789	3.325668155702684
>100	0.03760930203403642	3.83212566259787
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	293	0.2035556234846222	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	257	0.1785453762305389	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	241	0.16742971078427968	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	238	0.16534552351310608	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	235	0.16326133624193245	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	233	0.16187187806115005	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	221	0.15353512897645563	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	205	0.1424194635301964	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	204	0.14172473443980518	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	184	0.12783015263198116	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	179	0.12435650718002515	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	169	0.11740921627611313	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	160	0.11115665446259232	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	152	0.1055988217394627	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	148	0.1028199053778979	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	6.94729090391202E-4	0.0
15	0.0	0.0	0.0	6.94729090391202E-4	0.0
16	0.0	0.0	0.0	6.94729090391202E-4	0.0
17	0.0	0.0	0.0	6.94729090391202E-4	0.0
18	0.0	0.0	0.0	0.001389458180782404	0.0
19	0.0	0.0	0.0	0.0020841872711736056	0.0
20	0.0	0.0	0.0	0.0020841872711736056	0.0
21	0.0	0.0	0.0	0.0034736454519560095	0.0
22	0.0	0.0	0.0	0.004168374542347211	0.0
23	0.0	0.0	0.0	0.005557832723129616	0.0
24	0.0	0.0	0.0	0.006947290903912019	0.0
25	0.0	0.0	0.0	0.007642019994303221	0.0
26	0.0	0.0	0.0	0.01042093635586803	0.0
27	0.0	0.0	0.0	0.03334699633877769	0.0
28	0.0	0.0	0.0	0.1062935508298539	0.0
29	0.0	0.0	0.0	0.24523936890809428	0.0
30	0.0	0.0	0.0	0.42864784877137163	0.0
31	0.0	0.0	0.0	0.9760943719996388	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	30	3.5839877E-4	30.833334	1
GGTATCA	170	0.0	30.470589	1
ATTGTAG	25	0.0054805772	29.599998	3
CCGCTTA	25	0.0054805772	29.599998	25
CTAAACC	25	0.0054805772	29.599998	4
GCTTATT	25	0.0054805772	29.599998	27
ACGTGTG	25	0.0054805772	29.599998	11
TCGTAAG	25	0.0054805772	29.599998	27
GTAAGGG	25	0.0054805772	29.599998	29
TTATTGA	60	4.267713E-8	27.750002	29
TGATTCC	55	6.197988E-7	26.90909	17
ATTGATA	55	6.197988E-7	26.90909	31
AAGAGTC	35	8.831587E-4	26.42857	7
CATTGTA	35	8.831587E-4	26.42857	2
GTGATTC	70	6.490154E-9	26.42857	16
CTTATTG	65	9.273026E-8	25.615385	28
GATTCCT	60	1.3238714E-6	24.666668	18
CTTATAC	160	0.0	24.281252	37
GATATGC	55	1.887707E-5	23.545454	34
AGCGTAC	40	0.0019230328	23.125002	1
>>END_MODULE