FastQCFastQC Report
Fri 10 Feb 2017
ERR1631853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15508
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA510.3288625225689967No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA380.24503482073768376No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA350.22568996646891928No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA280.1805519731751354No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT260.16765540366262574No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT260.16765540366262574No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC240.15475883415011607No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC240.15475883415011607No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG240.15475883415011607No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA240.15475883415011607No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA230.14831054939386124No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.1418622646376064No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG220.1418622646376064No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA220.1418622646376064No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA220.1418622646376064No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA220.1418622646376064No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC210.13541397988135156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT200.12896569512509673No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA200.12896569512509673No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA190.12251741036884188No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT190.12251741036884188RNA PCR Primer, Index 47 (95% over 22bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG180.11606912561258705No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT180.11606912561258705No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC170.10962084085633221No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC170.10962084085633221No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA170.10962084085633221No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC170.10962084085633221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170.10962084085633221No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA170.10962084085633221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA250.00533275829.61
GTATCAA502.5392877E-422.21
TATCAAC650.001486972817.0769252
ATCAACG700.002441170415.8571423
TCAACGC750.003866747714.84
CAACGCA800.005936841513.8755
AACGCAG850.008868895513.0588246
Produced by FastQC (version 0.11.5)