##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631853.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15508
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.626579829765284	31.0	31.0	34.0	30.0	34.0
2	31.825444931648182	31.0	31.0	34.0	30.0	34.0
3	31.90662883672943	33.0	31.0	34.0	30.0	34.0
4	35.63251225174103	37.0	35.0	37.0	33.0	37.0
5	35.17410368841888	37.0	35.0	37.0	32.0	37.0
6	35.18764508640702	37.0	35.0	37.0	32.0	37.0
7	35.21034304874903	37.0	35.0	37.0	32.0	37.0
8	35.120389476399275	37.0	35.0	37.0	32.0	37.0
9	36.846982202734075	38.0	37.0	39.0	33.0	39.0
10	36.684614392571575	38.0	35.0	39.0	32.0	39.0
11	36.738586535981426	38.0	37.0	39.0	32.0	39.0
12	36.67745679649213	38.0	35.0	39.0	32.0	39.0
13	36.745357234975494	38.0	37.0	39.0	33.0	39.0
14	37.852527727624455	39.0	37.0	40.0	33.0	41.0
15	37.77353623936033	39.0	37.0	40.0	33.0	41.0
16	37.69705958215115	39.0	37.0	40.0	33.0	41.0
17	37.62116327057003	39.0	37.0	40.0	33.0	41.0
18	37.64276502450348	39.0	37.0	40.0	33.0	41.0
19	37.647085375290175	39.0	37.0	40.0	33.0	41.0
20	37.672878514315194	39.0	37.0	40.0	32.0	41.0
21	37.562419396440546	39.0	37.0	40.0	32.0	41.0
22	37.520376579829765	39.0	37.0	40.0	32.0	41.0
23	37.42378127418107	39.0	37.0	40.0	32.0	41.0
24	37.43828991488264	39.0	36.0	40.0	32.0	41.0
25	37.350593242197576	39.0	36.0	40.0	32.0	41.0
26	37.27482589631158	39.0	36.0	40.0	32.0	41.0
27	37.167977817900436	39.0	36.0	40.0	31.0	41.0
28	37.13541397988135	39.0	36.0	40.0	31.0	41.0
29	37.1163915398504	39.0	36.0	40.0	31.0	41.0
30	36.99593758060356	39.0	36.0	40.0	31.0	41.0
31	36.8836084601496	39.0	36.0	40.0	31.0	41.0
32	36.84601496002063	38.0	36.0	40.0	31.0	41.0
33	36.73188031983492	38.0	35.0	40.0	31.0	41.0
34	36.72053133866392	38.0	35.0	40.0	31.0	41.0
35	36.51915140572608	38.0	35.0	40.0	30.0	41.0
36	36.45292752127934	38.0	35.0	40.0	30.0	41.0
37	36.39850399793655	38.0	35.0	40.0	30.0	41.0
38	36.38547846272891	38.0	35.0	40.0	30.0	41.0
39	36.27263347949445	38.0	35.0	40.0	30.0	41.0
40	36.01902244003095	38.0	35.0	40.0	29.0	41.0
41	36.03166107815321	38.0	35.0	40.0	30.0	41.0
42	35.99322930100593	38.0	35.0	40.0	29.0	41.0
43	35.375354655661596	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	2.0
21	1.0
22	17.0
23	14.0
24	27.0
25	36.0
26	69.0
27	106.0
28	145.0
29	240.0
30	301.0
31	407.0
32	487.0
33	697.0
34	962.0
35	1365.0
36	2133.0
37	3177.0
38	3687.0
39	1632.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.17771472788238	16.99767861748775	12.187258189321641	27.637348465308225
2	21.769409337116326	19.976786174877486	31.048491101367038	27.20531338663915
3	21.26644312612845	20.18313128707764	28.688418880577764	29.86200670621615
4	16.301263863812228	16.4044364199123	33.10549393861233	34.18880577766314
5	17.249161722981686	33.25380448800619	32.81532112458086	16.681712664431263
6	34.846530822801135	33.976012380706734	14.785916946092339	16.391539850399795
7	30.468145473304105	27.501934485426876	19.86716533402115	22.162754707247874
8	27.33427908176425	30.661594015991746	20.26695898890895	21.737167913335053
9	28.03714212019603	13.090018055197316	17.06860974980655	41.8042300748001
10	19.396440546814546	23.342790817642506	29.700799587309774	27.559969048233167
11	38.79288109362909	19.338405984008254	18.54526695898891	23.323445963373743
12	22.775341759092083	23.194480268248647	26.418622646376065	27.61155532628321
13	34.96259994841372	17.62316223884447	20.208924426102655	27.20531338663915
14	23.66520505545525	19.88006190353366	22.83982460665463	33.61490843435646
15	29.958730977559966	23.207376837761156	20.32499355171524	26.50889863296363
16	26.23807067320093	24.006964147536756	21.58885736394119	28.166107815321123
17	27.34717565127676	23.742584472530307	21.18261542429714	27.727624451895792
18	26.586278050038693	21.37606396698478	22.859169460923397	29.178488522053136
19	28.307970079958732	22.298168687129223	22.394892958473044	26.998968274439
20	29.488006190353367	20.686097498065514	22.67861748774826	27.14727882383286
21	28.37245292752128	21.530822801134896	21.820995615166368	28.275728656177456
22	29.023729687903018	22.001547588341502	20.91823574929069	28.056486974464796
23	28.55945318545267	21.86613360846015	21.704926489553777	27.869486716533405
24	27.927521279339697	21.595305648697448	22.58834150116069	27.888831570802164
25	28.10807325251483	21.846788754191383	23.06551457312355	26.979623420170235
26	28.056486974464796	22.3304101109105	22.3304101109105	27.282692803714216
27	27.572865617745677	21.89192674748517	23.729687903017798	26.805519731751353
28	26.973175135413978	22.581893216404435	21.775857621872582	28.669074026308998
29	27.314934227495485	23.155790559711118	21.92416817126644	27.605107041526956
30	27.044106267732783	21.653340211503743	23.252514831054942	28.05003868970854
31	28.50786690740263	21.69202992004127	22.743100335310807	27.057002837245292
32	26.244518957957187	22.143409852979108	22.381996388960538	29.23007480010317
33	26.618519473819962	22.091823574929066	23.316997678617486	27.972659272633482
34	26.399277792107302	21.81454733041011	23.632963631673974	28.153211245808613
35	26.425070931132318	22.930100593242198	24.50993035852463	26.134898117100853
36	26.715243745163786	22.317513541397986	23.819963889605365	27.14727882383286
37	26.792623162238844	22.620582924941964	23.66520505545525	26.921588857363943
38	27.179520247614136	21.653340211503743	24.419654371937064	26.74748516894506
39	25.741552746969305	21.31802940417849	24.587309775599692	28.353108073252514
40	25.806035594531856	21.930616456022697	25.806035594531856	26.457312354913594
41	24.16172298168687	20.892442610265668	26.74103688418881	28.204797523858655
42	23.38792881093629	21.530822801134896	27.559969048233167	27.521279339695642
43	23.046169718854784	21.382512251741037	27.353623936033017	28.217694093371165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.5
9	2.0
10	2.0
11	2.0
12	2.0
13	6.0
14	10.0
15	21.0
16	32.0
17	32.5
18	33.0
19	33.0
20	34.0
21	35.0
22	25.5
23	16.0
24	23.0
25	30.0
26	30.0
27	31.5
28	33.0
29	40.0
30	47.0
31	69.0
32	91.0
33	91.0
34	129.0
35	167.0
36	195.0
37	223.0
38	279.5
39	336.0
40	336.0
41	402.0
42	468.0
43	569.5
44	671.0
45	936.0
46	1201.0
47	1201.0
48	1251.0
49	1301.0
50	1499.5
51	1698.0
52	1708.5
53	1719.0
54	1719.0
55	1579.0
56	1439.0
57	1440.5
58	1442.0
59	1282.0
60	1122.0
61	1122.0
62	1075.0
63	1028.0
64	849.5
65	671.0
66	594.0
67	517.0
68	517.0
69	444.0
70	371.0
71	330.5
72	290.0
73	250.0
74	210.0
75	210.0
76	178.5
77	147.0
78	116.5
79	86.0
80	55.5
81	25.0
82	25.0
83	27.0
84	29.0
85	19.0
86	9.0
87	7.0
88	5.0
89	5.0
90	3.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	15508.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.44389992262059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.86677768526228	69.59633737425844
2	5.287260616153206	8.189321640443643
3	1.8151540383014155	4.2171782305906635
4	0.9825145711906744	3.043590404952283
5	0.5412156536219818	2.0956925457828217
6	0.34138218151540384	1.58627805003869
7	0.19983347210657784	1.0833118390508125
8	0.10824313072439634	0.670621614650503
9	0.18318068276436303	1.2767603817384576
>10	0.6661115736885929	7.912045395924684
>50	0.008326394671107412	0.3288625225689967
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	51	0.3288625225689967	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	38	0.24503482073768376	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	35	0.22568996646891928	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	28	0.1805519731751354	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	26	0.16765540366262574	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	26	0.16765540366262574	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	24	0.15475883415011607	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	24	0.15475883415011607	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	24	0.15475883415011607	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	24	0.15475883415011607	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	23	0.14831054939386124	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	22	0.1418622646376064	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	22	0.1418622646376064	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	22	0.1418622646376064	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	22	0.1418622646376064	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	22	0.1418622646376064	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	21	0.13541397988135156	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.12896569512509673	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	20	0.12896569512509673	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	19	0.12251741036884188	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	19	0.12251741036884188	RNA PCR Primer, Index 47 (95% over 22bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	18	0.11606912561258705	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	18	0.11606912561258705	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	17	0.10962084085633221	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	17	0.10962084085633221	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	17	0.10962084085633221	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	17	0.10962084085633221	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.10962084085633221	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	17	0.10962084085633221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0709311323188032	0.0
29	0.0	0.0	0.0	0.15475883415011607	0.0
30	0.0	0.0	0.0	0.29662109878772247	0.0
31	0.0	0.0	0.0	0.5738973433066804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25	0.005332758	29.6	1
GTATCAA	50	2.5392877E-4	22.2	1
TATCAAC	65	0.0014869728	17.076925	2
ATCAACG	70	0.0024411704	15.857142	3
TCAACGC	75	0.0038667477	14.8	4
CAACGCA	80	0.0059368415	13.875	5
AACGCAG	85	0.0088688955	13.058824	6
>>END_MODULE