FastQCFastQC Report
Fri 10 Feb 2017
ERR1631852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251811
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.6207036229553117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15190.6032302004280988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11260.44716076740094757No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10060.39950597869036697No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9030.35860228504711866No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8500.3375547533666123No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8430.33477489069182836No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7390.2934740738093253No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7090.2815603766316801No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6120.24303942242396084No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6110.24264229918470598No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5990.23787682031364793No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5820.2311257252463157No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5530.21960915130792538No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5490.218020658350906No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5420.21524079567612217No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5390.21404942595835766No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5270.20928394708729958No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5120.20332709849847702No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5080.2017386055414577No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4880.19379614075636092No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4790.1902220316030674No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4700.18664792244977385No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4670.18545655273200934No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4650.18466230625349964No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4480.1779112111861674No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4380.17393997879361903No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4360.17314573231510935No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4340.17235148583659968No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4300.17076299287958033No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4260.16917449992256095No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4100.16282052809448355No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4050.16083491189820937No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4050.16083491189820937No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4010.15924641894119002No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3980.1580550492234255No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3940.15646655626640615No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3910.15527518654864164No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3880.15408381683087713No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3850.15289244711311262No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3840.15249532387385778No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3800.15090683091683843No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3760.14931833795981908No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3680.14614135204578038No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3550.1409787499354675No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3530.1401845034569578No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3430.13621327106440942No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3340.13263916191111588No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3330.13224203867186105No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3320.1318449154326062No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3310.13144779219335134No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3300.13105066895409653No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA3220.12787368304005783No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3210.12747655980080297No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3190.12668231332229332No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3180.12628519008303846No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3180.12628519008303846No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3170.12588806684378362No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA3150.12509382036527394No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3150.12509382036527394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.12469669712601912No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3110.1235053274082546No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3060.12151971121198042No Hit
GGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTAC3040.12072546473347073No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2990.11873984853719653No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA2980.11834272529794171No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2960.11754847881943205No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2940.11675423234092236No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2910.11556286262315785No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2870.1139743696661385No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2830.11238587670911915No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2810.11159163023060946No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC2780.11040026051284495No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2780.11040026051284495No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA2710.10762039783806109No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2690.1068261513595514No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2690.1068261513595514No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2670.10603190488104174No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2650.10523765840253206No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC2590.10285491896700302No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC2580.1024577957277482No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2550.10126642600998367No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2540.10086930277072885No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG2530.100472179531474No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2520.10007505629221916No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2520.10007505629221916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGAGC200.001839207637.033
ATACGGC352.3809607E-531.71428729
TCGTTGC250.00548829529.616
TTTACTG250.00548829529.622
GCCTAGT250.00548829529.61
CTACGGG405.9220023E-527.75000211
GGTATCA7600.027.0197371
GCAGCGT556.2291656E-726.909091
TAACCCT451.3192068E-424.66666835
ACTCCTA451.3192068E-424.6666687
GGGCAGT1300.024.1923087
GCAGTCG1350.023.2962979
CAGTCGG1400.022.46428710
TGGGCAG1400.022.4642876
CCTACGG502.6939894E-422.210
CTCCTAC502.6939894E-422.28
GAGGCAG502.6939894E-422.217
AGTCGGT1350.021.92592611
TTAGATA705.075857E-621.1428594
GGCAGCA555.12778E-420.18181819
Produced by FastQC (version 0.11.5)