FastQCFastQC Report
Fri 10 Feb 2017
ERR1631851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18098
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA560.30942645596198476No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT450.2486462592551663No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA440.24312078682727375No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA430.23759531439938114No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC350.19339153497624045No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA340.1878660625483479No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA310.17128964526467014No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA310.17128964526467014No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC280.15471322798099238No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA260.1436622831252072No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT250.13813681069731462No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT240.13261133826942204No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG240.13261133826942204No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA220.12156039341363688No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA220.12156039341363688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210.11603492098574428No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA210.11603492098574428No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG210.11603492098574428No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA210.11603492098574428No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT210.11603492098574428No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC200.11050944855785169No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT190.10498397612995912No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC190.10498397612995912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGACC200.001794361537.04
AACGCAG451.2541265E-424.6666667
CAACGCA451.2541265E-424.6666666
ACTTCAT400.001858922123.12500231
TCAACGC502.562135E-422.1999995
TATCAAC502.562135E-422.1999993
ATCAACG502.562135E-422.1999994
CTTCATG450.003683986620.55555532
GAGGCAA450.003683986620.55555520
GGCAATC450.003683986620.55555522
CACCGAG450.003683986620.55555516
CGAGGCA450.003683986620.55555519
ACCGAGG450.003683986620.55555517
AGTACGG450.003683986620.55555514
GAGTACG450.003683986620.55555513
TCGCACT450.003683986620.55555527
CCGAGGC450.003683986620.55555518
AGGCAAT450.003683986620.55555521
CGCACTT450.003683986620.55555528
CCCCAAT450.003683986620.5555551
Produced by FastQC (version 0.11.5)