##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631851.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18098
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.103878881644384	33.0	31.0	34.0	30.0	34.0
2	32.29622057685932	34.0	31.0	34.0	30.0	34.0
3	32.36650458614211	34.0	31.0	34.0	30.0	34.0
4	35.97690352525141	37.0	35.0	37.0	35.0	37.0
5	35.805061332743946	37.0	35.0	37.0	35.0	37.0
6	35.813460050834344	37.0	35.0	37.0	35.0	37.0
7	35.81848823074373	37.0	35.0	37.0	35.0	37.0
8	35.77732346115593	37.0	35.0	37.0	35.0	37.0
9	37.55757542269864	39.0	37.0	39.0	35.0	39.0
10	37.42756105647033	39.0	37.0	39.0	35.0	39.0
11	37.50989059564593	39.0	37.0	39.0	35.0	39.0
12	37.38291523925296	39.0	37.0	39.0	35.0	39.0
13	37.50160238700409	39.0	37.0	39.0	35.0	39.0
14	38.74842524035805	40.0	38.0	41.0	35.0	41.0
15	38.72974914355177	40.0	38.0	41.0	35.0	41.0
16	38.651895237042766	40.0	38.0	41.0	35.0	41.0
17	38.63460050834346	40.0	38.0	41.0	35.0	41.0
18	38.6030500607802	40.0	38.0	41.0	35.0	41.0
19	38.644380594540834	40.0	38.0	41.0	35.0	41.0
20	38.608907061553765	40.0	38.0	41.0	35.0	41.0
21	38.57199690573544	40.0	38.0	41.0	34.0	41.0
22	38.56741076362029	40.0	38.0	41.0	34.0	41.0
23	38.49845286772019	40.0	38.0	41.0	34.0	41.0
24	38.47712454414852	40.0	38.0	41.0	34.0	41.0
25	38.41286329981213	40.0	38.0	41.0	34.0	41.0
26	38.32633440159134	40.0	38.0	41.0	34.0	41.0
27	38.13874461266438	40.0	38.0	41.0	34.0	41.0
28	38.13222455519947	40.0	38.0	41.0	34.0	41.0
29	38.08277157696983	40.0	38.0	41.0	33.0	41.0
30	38.08669466239363	40.0	38.0	41.0	33.0	41.0
31	37.9640844292187	40.0	37.0	41.0	33.0	41.0
32	37.906564261244334	40.0	37.0	41.0	33.0	41.0
33	37.85539838656205	40.0	37.0	41.0	33.0	41.0
34	37.78826389656316	40.0	37.0	41.0	33.0	41.0
35	37.715493424687814	40.0	37.0	41.0	33.0	41.0
36	37.6512874350757	40.0	37.0	41.0	33.0	41.0
37	37.62852248867278	40.0	37.0	41.0	33.0	41.0
38	37.578351199027516	39.0	37.0	41.0	33.0	41.0
39	37.47988728036247	39.0	36.0	41.0	33.0	41.0
40	37.17598629682838	39.0	36.0	41.0	31.0	41.0
41	37.21085202784838	39.0	36.0	40.0	31.0	41.0
42	37.15559730356946	39.0	36.0	40.0	32.0	41.0
43	36.63255608354514	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	1.0
21	2.0
22	8.0
23	7.0
24	19.0
25	40.0
26	62.0
27	84.0
28	91.0
29	139.0
30	189.0
31	307.0
32	363.0
33	535.0
34	700.0
35	999.0
36	1646.0
37	2806.0
38	5580.0
39	4517.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.20366891369212	16.43828047298044	12.283125207205217	28.074925406122226
2	21.52724057906951	19.416510111614542	30.83766162006852	28.218587689247432
3	21.57144435849265	19.372306332191403	28.765609459608797	30.29063984970715
4	16.3940766935573	15.642612443363907	33.56171952701956	34.401591336059234
5	17.42181456514532	32.462150513868934	32.68316941098464	17.432865510001104
6	35.280141452094156	33.22466570891811	14.979555752016799	16.515637086970937
7	31.301801304011494	27.16874792794784	19.24522046634987	22.284230301690794
8	28.13018013040115	30.384572880981324	20.41109514863521	21.07415183998232
9	28.88164438059454	12.299701624488895	17.449441927284784	41.369212067631786
10	19.676207315725495	23.052270969167864	29.771245441485245	27.500276273621395
11	40.19781191291855	18.416399602165985	18.825284561830035	22.560503923085424
12	23.057796441595755	23.090949276163112	26.301248756768704	27.550005525472425
13	35.517736766493535	17.084760747043873	20.22322908608686	27.174273400375732
14	23.853464471212288	19.659630898441815	22.477621836667037	34.00928279367886
15	30.00884075588463	23.698751243231296	19.676207315725495	26.61620068515858
16	25.82053265554205	23.98607580948171	21.93612553873356	28.25726599624268
17	27.157696983092055	23.179356835009393	21.637750027627362	28.025196154271192
18	27.992043319703836	20.43872251077467	22.775997347773234	28.79323682174826
19	28.48933583821417	21.576969830920547	22.59365675765278	27.340037573212513
20	29.622057685932145	20.300585700077356	22.814675654768482	27.262680959222013
21	27.373190407779862	22.21239916012819	21.74273400375732	28.671676428334624
22	28.48933583821417	22.632335064648025	21.09625372969389	27.782075367443916
23	28.218587689247432	21.31727262680959	21.88639628688253	28.577743397060452
24	28.88716985302243	21.134932036689136	22.135042546137697	27.84285556415074
25	28.091501823405903	21.32832357166538	22.94176152061001	27.638413084318707
26	28.859542490882973	22.284230301690794	22.007956680296164	26.84827052713007
27	27.522378163332966	21.73720853132943	23.201458724720965	27.538954580616643
28	26.599624267874905	22.5770803403691	21.37805282351641	29.445242568239582
29	27.715769698309206	23.079898331307326	21.637750027627362	27.56658194275611
30	27.588683832467677	21.626699082771577	22.3560614432534	28.428555641507348
31	28.24621505138689	22.372637860537075	22.14609349099348	27.235053597082548
32	26.544369543595973	21.560393413636866	22.389214277820756	29.506022764946405
33	26.8261686374185	21.168084871256493	24.168416399602165	27.837330091722844
34	27.041662062106308	21.4609349099348	22.969388882749474	28.52801414520941
35	26.367554425903418	22.70416620621063	24.533097579843076	26.395181788042876
36	26.411758205326556	22.466570891811248	23.94187203005857	27.179798872803623
37	26.770913913139573	21.720632114045753	24.251298486020556	27.257155486794122
38	26.201790253066637	21.86981986959885	24.096585258039564	27.831804619294946
39	26.33440159133606	21.173610343684384	24.98618631893027	27.505801746049286
40	25.96972041109515	21.748259476185215	25.765277931263125	26.516742181456515
41	24.168416399602165	21.24544148524699	27.19084981765941	27.395292297491437
42	23.074372858879435	21.389103768372195	27.610785722179248	27.925737650569122
43	22.15161896342137	21.786937783180463	27.57763288761189	28.483810365786276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.5
7	2.0
8	1.0
9	0.0
10	2.0
11	4.0
12	4.0
13	7.5
14	11.0
15	20.0
16	29.0
17	28.5
18	28.0
19	28.0
20	34.0
21	40.0
22	31.0
23	22.0
24	27.0
25	32.0
26	32.0
27	29.0
28	26.0
29	38.0
30	50.0
31	89.0
32	128.0
33	128.0
34	155.0
35	182.0
36	210.5
37	239.0
38	295.0
39	351.0
40	351.0
41	431.5
42	512.0
43	603.5
44	695.0
45	985.5
46	1276.0
47	1276.0
48	1415.0
49	1554.0
50	1747.0
51	1940.0
52	1965.5
53	1991.0
54	1991.0
55	1860.0
56	1729.0
57	1714.5
58	1700.0
59	1602.0
60	1504.0
61	1504.0
62	1382.0
63	1260.0
64	1070.5
65	881.0
66	768.5
67	656.0
68	656.0
69	561.0
70	466.0
71	387.5
72	309.0
73	245.5
74	182.0
75	182.0
76	146.0
77	110.0
78	88.5
79	67.0
80	60.0
81	53.0
82	53.0
83	40.5
84	28.0
85	25.5
86	23.0
87	15.0
88	7.0
89	7.0
90	7.5
91	8.0
92	4.0
93	0.0
94	1.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	18098.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.77666040446458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.70631970260223	71.45540943750692
2	4.888826541348109	7.7025085644822635
3	1.7324822894016976	4.0943750690684055
4	0.8557199971943606	2.696430544811581
5	0.4909868836361086	1.9339153497624046
6	0.2384793434803956	1.1271963752900873
7	0.2174370484674195	1.199027516852691
8	0.17535245844146735	1.105094485578517
9	0.09118327838956301	0.6464802740634324
>10	0.596198358700989	7.730135926621726
>50	0.007014098337658693	0.30942645596198476
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	56	0.30942645596198476	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	45	0.2486462592551663	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	44	0.24312078682727375	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	43	0.23759531439938114	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	35	0.19339153497624045	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	34	0.1878660625483479	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	31	0.17128964526467014	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	31	0.17128964526467014	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	28	0.15471322798099238	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	26	0.1436622831252072	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	25	0.13813681069731462	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	24	0.13261133826942204	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	24	0.13261133826942204	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	22	0.12156039341363688	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	22	0.12156039341363688	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21	0.11603492098574428	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	21	0.11603492098574428	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	21	0.11603492098574428	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	21	0.11603492098574428	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	21	0.11603492098574428	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	20	0.11050944855785169	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	19	0.10498397612995912	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	19	0.10498397612995912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.005525472427892585	0.0
23	0.0	0.0	0.0	0.005525472427892585	0.0
24	0.0	0.0	0.0	0.005525472427892585	0.0
25	0.0	0.0	0.0	0.01105094485578517	0.0
26	0.0	0.0	0.0	0.01105094485578517	0.0
27	0.0	0.0	0.0	0.03315283456735551	0.0
28	0.0	0.0	0.0	0.08288208641838878	0.0
29	0.0	0.0	0.0	0.19339153497624048	0.0
30	0.0	0.0	0.0	0.32047740081776993	0.0
31	0.0	0.0	0.0	0.6409548016355399	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGACC	20	0.0017943615	37.0	4
AACGCAG	45	1.2541265E-4	24.666666	7
CAACGCA	45	1.2541265E-4	24.666666	6
ACTTCAT	40	0.0018589221	23.125002	31
TCAACGC	50	2.562135E-4	22.199999	5
TATCAAC	50	2.562135E-4	22.199999	3
ATCAACG	50	2.562135E-4	22.199999	4
CTTCATG	45	0.0036839866	20.555555	32
GAGGCAA	45	0.0036839866	20.555555	20
GGCAATC	45	0.0036839866	20.555555	22
CACCGAG	45	0.0036839866	20.555555	16
CGAGGCA	45	0.0036839866	20.555555	19
ACCGAGG	45	0.0036839866	20.555555	17
AGTACGG	45	0.0036839866	20.555555	14
GAGTACG	45	0.0036839866	20.555555	13
TCGCACT	45	0.0036839866	20.555555	27
CCGAGGC	45	0.0036839866	20.555555	18
AGGCAAT	45	0.0036839866	20.555555	21
CGCACTT	45	0.0036839866	20.555555	28
CCCCAAT	45	0.0036839866	20.555555	1
>>END_MODULE