FastQCFastQC Report
Fri 10 Feb 2017
ERR1631850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16391
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA550.3355499969495455No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA370.2257336343115124No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC350.21353181624061984No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG340.20743090720517354No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA300.18302727106338845No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT300.18302727106338845No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA290.17692636202794215No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT260.15862363492160333No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC250.15252272588615703No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA240.14642181685071076No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA230.1403209078152645No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA230.1403209078152645No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA230.1403209078152645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT220.1342199987798182No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA210.12811908974437192No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG210.12811908974437192No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA200.12201818070892562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT200.12201818070892562No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT190.11591727167347934No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC190.11591727167347934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT180.10981636263803307No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA170.10371545360258677No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC170.10371545360258677No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT170.10371545360258677No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA170.10371545360258677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCT1200.006666417710.79166637
Produced by FastQC (version 0.11.5)