##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631850.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16391
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11976084436581	31.0	31.0	33.0	28.0	34.0
2	31.35733024220609	31.0	31.0	34.0	30.0	34.0
3	31.412909523519005	31.0	31.0	34.0	30.0	34.0
4	35.2864376792142	37.0	35.0	37.0	33.0	37.0
5	34.44725764138857	35.0	35.0	37.0	30.0	37.0
6	34.38539442376914	35.0	35.0	37.0	30.0	37.0
7	34.449209932279906	35.0	35.0	37.0	30.0	37.0
8	34.38197791470929	35.0	35.0	37.0	30.0	37.0
9	35.93807577329022	37.0	35.0	39.0	32.0	39.0
10	35.64443902141419	37.0	35.0	39.0	30.0	39.0
11	35.773290220242814	37.0	35.0	39.0	30.0	39.0
12	35.744432920505155	37.0	35.0	39.0	30.0	39.0
13	35.826490147031905	37.0	35.0	39.0	30.0	39.0
14	36.72570312976634	38.0	36.0	40.0	31.0	41.0
15	36.62064547617595	38.0	36.0	40.0	31.0	41.0
16	36.522969922518456	38.0	35.0	40.0	31.0	41.0
17	36.521322677078885	38.0	36.0	40.0	31.0	41.0
18	36.49588188640107	38.0	36.0	40.0	31.0	41.0
19	36.56097858580929	38.0	35.0	40.0	31.0	41.0
20	36.54902080409981	38.0	35.0	40.0	31.0	41.0
21	36.4989323409188	38.0	35.0	40.0	31.0	41.0
22	36.44853883228601	38.0	35.0	40.0	30.0	41.0
23	36.35641510585077	38.0	35.0	40.0	30.0	41.0
24	36.324446342505034	38.0	35.0	40.0	30.0	41.0
25	36.236471234213894	38.0	35.0	40.0	30.0	41.0
26	36.08474162650235	38.0	35.0	40.0	30.0	41.0
27	35.89170886462083	38.0	34.0	40.0	29.0	41.0
28	35.94374961869318	38.0	34.0	40.0	30.0	41.0
29	35.87102678299067	38.0	34.0	40.0	29.0	41.0
30	35.824415837959855	38.0	34.0	40.0	29.0	41.0
31	35.64151058507718	38.0	34.0	40.0	29.0	41.0
32	35.622475748886586	38.0	34.0	40.0	29.0	41.0
33	35.472881459337444	38.0	34.0	40.0	28.0	40.0
34	35.45933744127875	38.0	34.0	40.0	28.0	41.0
35	35.27948264291379	38.0	34.0	40.0	27.0	40.0
36	35.28051979744982	38.0	34.0	40.0	27.0	40.0
37	35.20309926179001	38.0	33.0	40.0	27.0	40.0
38	35.15362088951254	38.0	33.0	40.0	27.0	40.0
39	35.06107009944482	37.0	33.0	39.0	27.0	40.0
40	34.80470990177537	37.0	33.0	39.0	26.0	40.0
41	34.794887438228294	37.0	33.0	39.0	26.0	40.0
42	34.69257519370386	37.0	33.0	39.0	26.0	40.0
43	34.06473064486609	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	2.0
20	3.0
21	20.0
22	21.0
23	30.0
24	45.0
25	70.0
26	142.0
27	192.0
28	224.0
29	328.0
30	476.0
31	630.0
32	818.0
33	1187.0
34	1676.0
35	2143.0
36	2720.0
37	2783.0
38	2175.0
39	704.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.46177780489293	17.717039838936003	13.153559880422183	27.66762247574889
2	20.09639436276005	20.340430724177903	33.018119699835275	26.54505521322677
3	20.267219815752547	21.072539808431458	29.229455188823135	29.430785186992864
4	15.68543713013239	16.441949850527728	35.17784149838326	32.69477152095662
5	15.654932584955159	34.482337868342384	33.84784332865597	16.014886218046488
6	33.152339698615094	35.37307058751754	15.447501677749983	16.02708803611738
7	30.40082972362882	28.424135196144224	20.45024708681594	20.724787993411017
8	26.898907937282658	32.51174424989323	20.56006344945397	20.02928436337014
9	28.39973156000244	12.519065340735768	17.631627112439755	41.44957598682203
10	18.485754377402234	24.690378866451102	30.425233359770605	26.39863339637606
11	39.11902873528156	19.943871636873894	18.7846989201391	22.152400707705446
12	22.71978524800195	24.6110670489903	27.7225306570679	24.946617045939846
13	34.84839241046916	18.19291074370081	21.389787078274665	25.568909767555365
14	23.58001342199988	21.029833445183332	23.44579342322006	31.944359709596732
15	29.522298822524558	23.97047160026844	20.828503447013606	25.678726130193397
16	25.318772497102067	24.867305228479044	22.365932523946068	27.44798975047282
17	26.929412482459885	24.43414068696236	21.816850710755904	26.819596119821853
18	26.606064303581235	21.822951619791347	23.439692514184614	28.131291562442804
19	28.77798792020011	22.506253431761333	23.49460069550363	25.221157952534927
20	30.217802452565433	21.18235617106949	22.933317064242573	25.666524312122508
21	26.923311573424442	22.3171252516625	22.73808797510829	28.02147519980477
22	28.00927338173388	23.360380696723812	21.585016167408945	27.04532975413337
23	27.4052833872247	22.994326154597037	23.012628881703375	26.587761576474893
24	27.20395338905497	22.469647977548654	23.372582514794704	26.95381611860167
25	27.539503386004515	21.944969800500274	24.482947959245926	26.032578854249284
26	27.252760661338538	23.354279787688366	23.152949789518637	26.24000976145446
27	26.63656884875846	22.451345250442316	24.00707705448112	26.9050088463181
28	25.7580379476542	23.9521688731621	22.420840705265086	27.86895247391861
29	26.2461106704899	24.385333414678787	22.85400524678177	26.51455066804954
30	26.124092489780974	23.024830699774267	23.39698615093649	27.454090659508267
31	27.570007931181745	22.829601610639987	23.08583979012873	26.51455066804954
32	25.733634311512414	23.220059788908546	22.71978524800195	28.326520651577088
33	25.648221585016167	22.878408882923555	24.964919773046184	26.50844975901409
34	26.502348849978645	22.292721615520712	24.458544323104142	26.7463852113965
35	24.891708864620828	24.519553413458606	24.958818864010738	25.62991885790983
36	25.520102495271797	23.360380696723812	25.184552498322248	25.93496430968214
37	26.63046793972302	22.915014337136235	24.238911597828075	26.215606125312675
38	25.654322494051613	23.098041608199622	24.495149777316822	26.752486120431946
39	24.507351595387714	21.438594350558233	26.130193398816427	27.923860655237632
40	25.105240680861446	21.98157525471295	26.14849612592276	26.764687938502835
41	23.58001342199988	21.792447074614117	27.734732475138795	26.892807028247205
42	22.262217070343482	21.963272527606613	28.338722469647976	27.43578793240193
43	22.13409798059911	21.847355255933135	28.930510646086265	27.088036117381492
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	1.5
9	1.0
10	2.0
11	3.0
12	3.0
13	7.0
14	11.0
15	21.5
16	32.0
17	35.0
18	38.0
19	38.0
20	41.5
21	45.0
22	34.0
23	23.0
24	29.0
25	35.0
26	35.0
27	42.0
28	49.0
29	71.5
30	94.0
31	121.5
32	149.0
33	149.0
34	210.5
35	272.0
36	294.5
37	317.0
38	405.5
39	494.0
40	494.0
41	552.0
42	610.0
43	683.0
44	756.0
45	1045.0
46	1334.0
47	1334.0
48	1447.0
49	1560.0
50	1749.5
51	1939.0
52	1926.0
53	1913.0
54	1913.0
55	1739.0
56	1565.0
57	1433.0
58	1301.0
59	1217.0
60	1133.0
61	1133.0
62	1027.5
63	922.0
64	756.0
65	590.0
66	505.0
67	420.0
68	420.0
69	354.0
70	288.0
71	237.0
72	186.0
73	150.0
74	114.0
75	114.0
76	103.5
77	93.0
78	66.0
79	39.0
80	34.5
81	30.0
82	30.0
83	22.0
84	14.0
85	10.5
86	7.0
87	7.5
88	8.0
89	8.0
90	5.5
91	3.0
92	1.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	16391.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.71447745714111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.76228378998928	72.35068025135746
2	4.875248737180468	7.772558111158563
3	1.7373335374253789	4.154719053138918
4	1.0255625287004437	3.2700872429992067
5	0.42093984386958516	1.6777499847477273
6	0.28317771314863005	1.3544018058690745
7	0.13010867901423542	0.7260081752181075
8	0.10714832389407623	0.6833018119699835
9	0.09949487218735649	0.713806357147215
>10	0.5510485228838206	6.961137209444207
>50	0.007653451706719731	0.3355499969495455
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	55	0.3355499969495455	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	37	0.2257336343115124	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	35	0.21353181624061984	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	34	0.20743090720517354	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	30	0.18302727106338845	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	30	0.18302727106338845	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	29	0.17692636202794215	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	26	0.15862363492160333	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	25	0.15252272588615703	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	24	0.14642181685071076	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	23	0.1403209078152645	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	23	0.1403209078152645	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	23	0.1403209078152645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.1342199987798182	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	21	0.12811908974437192	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	21	0.12811908974437192	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	20	0.12201818070892562	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.12201818070892562	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	19	0.11591727167347934	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	19	0.11591727167347934	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.10981636263803307	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	17	0.10371545360258677	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	17	0.10371545360258677	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	17	0.10371545360258677	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	17	0.10371545360258677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.012201818070892562	0.0
22	0.0	0.0	0.0	0.012201818070892562	0.0
23	0.0	0.0	0.0	0.012201818070892562	0.0
24	0.0	0.0	0.0	0.012201818070892562	0.0
25	0.0	0.0	0.0	0.012201818070892562	0.0
26	0.0	0.0	0.0	0.018302727106338845	0.0
27	0.0	0.0	0.0	0.04270636324812397	0.0
28	0.0	0.0	0.0	0.0976145445671405	0.0
29	0.0	0.0	0.0	0.2013299981697273	0.0
30	0.0	0.0	0.0	0.3233481788786529	0.0
31	0.0	0.0	0.0	0.6833018119699835	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCTCT	120	0.0066664177	10.791666	37
>>END_MODULE