FastQCFastQC Report
Fri 10 Feb 2017
ERR1631849.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631849.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982719
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25730.2618245907527991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.2502241230707863No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA23560.2397429987615992No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA23090.23496034980497985No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21380.21755964828196056No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21090.21460865211723798No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20580.20941896920686381No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20540.209011935253109No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA20050.20402576931961222No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC18970.1930358525682316No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT18930.19262881861447678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18680.19008485640350906No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC18100.1841828640740639No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA16360.16647688708572847No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT15170.15436762696152206No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13500.1373739593922576No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC13350.13584758206567696No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13290.1352370311350447No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA13120.13350713683158666No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG12970.131980759505006No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12630.1285209708980899No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC12600.12821569543277378No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12600.12821569543277378No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA12510.12729986903682539No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA12400.12618052566399957No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12220.1243488728721028No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA11910.12119435973050281No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11880.12088908426518669No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT11480.11681874472763833No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11360.11559764286637382No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10770.10959389204848996No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10700.108881582629419No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10450.10633762041845127No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA10360.10542179402250287No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC10010.10186024692714804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.029.112851
AGTTCCG551.9022946E-523.54545432
GTTCTAC652.682189E-622.769231
CCGCTTA1400.022.46428525
ATACGGC1005.347829E-1022.229
TTTTACG806.957998E-720.81254
GCGCGAA450.003825419820.55555511
ATAGACT555.142364E-420.1818184
TAGCGAA656.900829E-519.92307710
TCGGGTA656.900829E-519.92307725
GCGGTAA6950.019.16546823
GTATCAA25000.018.5742
GTAAGTT801.616282E-518.529
CGCGGTA7350.018.3741522
CTTATAC9000.018.29444537
TACGGCT1258.572897E-917.7630
GCAGTCG4200.017.619059
TATACAC3050.017.59016437
CTTTATT3400.017.4117642
CCCCTAT752.0668822E-417.2666661
Produced by FastQC (version 0.11.5)