##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631849.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	982719
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.649581416457806	34.0	31.0	34.0	31.0	34.0
2	32.809190623158806	34.0	31.0	34.0	31.0	34.0
3	32.89894873305594	34.0	31.0	34.0	31.0	34.0
4	36.30535381935223	37.0	37.0	37.0	35.0	37.0
5	36.226124660253845	37.0	35.0	37.0	35.0	37.0
6	36.28372505263458	37.0	37.0	37.0	35.0	37.0
7	36.277652106044556	37.0	37.0	37.0	35.0	37.0
8	36.26161191551196	37.0	37.0	37.0	35.0	37.0
9	38.08630747955418	39.0	38.0	39.0	37.0	39.0
10	38.030309783366356	39.0	38.0	39.0	35.0	39.0
11	38.09632763791073	39.0	38.0	39.0	37.0	39.0
12	38.0384148469705	39.0	38.0	39.0	35.0	39.0
13	38.09523271657513	39.0	38.0	39.0	37.0	39.0
14	39.49046573842573	41.0	39.0	41.0	37.0	41.0
15	39.500170445468136	41.0	39.0	41.0	37.0	41.0
16	39.442143685020845	41.0	39.0	41.0	37.0	41.0
17	39.425449187407594	41.0	39.0	41.0	37.0	41.0
18	39.45464675049531	41.0	39.0	41.0	37.0	41.0
19	39.46525202016039	41.0	39.0	41.0	37.0	41.0
20	39.47620632144082	41.0	39.0	41.0	37.0	41.0
21	39.433675343612975	41.0	39.0	41.0	37.0	41.0
22	39.40898262880844	41.0	39.0	41.0	37.0	41.0
23	39.35091414738089	41.0	39.0	41.0	36.0	41.0
24	39.357189593362904	41.0	39.0	41.0	36.0	41.0
25	39.30769629975609	41.0	39.0	41.0	36.0	41.0
26	39.2413009212196	40.0	39.0	41.0	36.0	41.0
27	39.130221355239904	40.0	39.0	41.0	36.0	41.0
28	39.127872769326736	40.0	39.0	41.0	35.0	41.0
29	39.110323500410594	40.0	39.0	41.0	35.0	41.0
30	39.06269849265151	40.0	39.0	41.0	35.0	41.0
31	38.96025516958561	40.0	38.0	41.0	35.0	41.0
32	38.91339335048981	40.0	38.0	41.0	35.0	41.0
33	38.837306493514426	40.0	38.0	41.0	35.0	41.0
34	38.81840485428693	40.0	38.0	41.0	35.0	41.0
35	38.73682303893585	40.0	38.0	41.0	35.0	41.0
36	38.684936385680956	40.0	38.0	41.0	35.0	41.0
37	38.63189070324274	40.0	38.0	41.0	35.0	41.0
38	38.587947317595365	40.0	38.0	41.0	35.0	41.0
39	38.502471204891734	40.0	38.0	41.0	35.0	41.0
40	38.1715037564146	40.0	38.0	41.0	34.0	41.0
41	38.22787388867011	40.0	37.0	41.0	34.0	41.0
42	38.20491005058415	40.0	37.0	41.0	34.0	41.0
43	37.73930696363864	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	0.0
16	2.0
17	2.0
18	4.0
19	16.0
20	40.0
21	53.0
22	122.0
23	218.0
24	441.0
25	767.0
26	1295.0
27	2070.0
28	3206.0
29	4586.0
30	6463.0
31	9024.0
32	11957.0
33	16426.0
34	23603.0
35	36210.0
36	55089.0
37	101382.0
38	227117.0
39	482622.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.341484188257276	16.491692945796306	12.910506462172808	27.25631640377361
2	20.80564230466695	19.662690962523367	32.08709712542446	27.444569607385223
3	21.81783398916679	19.885847327669456	29.245898369727257	29.050420313436497
4	15.44205413755102	16.21399403084707	34.26737449871224	34.07657733288967
5	16.896589971293928	32.57452028504588	33.68043153739777	16.84845820626242
6	33.42206673525189	33.508052657982596	16.035204366660256	17.03467624010526
7	29.989752920214226	27.73091799385175	20.487748786784422	21.791580299149604
8	27.858421379865455	29.474956727202787	20.697981823898797	21.968640069032958
9	28.395400923356522	13.204588493760678	18.490738451174753	39.90927213170804
10	19.94069515293792	23.511604029229108	29.694755062230406	26.85294575560257
11	38.09959917331404	20.144517405280656	19.22879276782071	22.527090653584594
12	23.03445847694	24.080026945647738	26.72350895830853	26.162005619103734
13	35.33146301231583	17.79145411862394	21.140020697676547	25.73706217138368
14	23.88353130447259	20.876669729597168	23.971043604529882	31.26875536140036
15	29.48604840244261	24.75102241841259	20.371540592987415	25.391388586157387
16	24.49174178987076	24.565109660035066	23.38613581298418	27.55701273711
17	26.425560104159988	23.84740704107685	22.492187492050117	27.234845362713045
18	26.327973713747266	21.391160647143284	24.22035190120472	28.06051373790473
19	27.77752338155668	22.624270010043563	24.33299854790637	25.26520806049339
20	29.79702234311131	20.915134438227	23.228206638927304	26.05963657973439
21	27.179183469537072	22.380049637790663	23.052673246370528	27.38809364630174
22	27.780881411675157	23.05989809904968	22.589977399439718	26.56924308983545
23	27.618881898080733	22.129825514719876	23.13316421072555	27.118128376473845
24	27.122402232988268	21.79504008775652	24.249658345875066	26.832899333380144
25	27.223550170496345	22.513760291599123	23.900728489018732	26.361961048885796
26	27.27544699960009	23.13143431642209	23.341769112024902	26.25134957195292
27	26.36043467155921	21.911044764576648	25.091709837705388	26.63681072615875
28	25.81277048678208	23.350316825053756	22.778739395493524	28.05817329267064
29	26.84338045768933	23.78502908766392	23.121156709089778	26.250433745556972
30	26.880624064457898	22.16981660067629	23.92952614124689	27.020033193618932
31	26.96376074951232	22.97218228201551	23.78350271033734	26.28055425813483
32	25.163144296589362	22.56229909058439	23.618145166624437	28.656411446201812
33	24.90294784165158	22.689497201132774	25.606709547693697	26.80084540952195
34	26.1519315287483	22.002932679636803	24.3417497779121	27.503386013702798
35	25.052736336633362	23.805279026863225	24.921874920501182	26.22010971600223
36	25.55369337521713	23.082895517436825	25.45407181503563	25.909339292310417
37	26.1816450073724	22.74668547163533	25.12457782947109	25.94709169152118
38	25.70806100217865	22.2034986603495	25.025872095685543	27.062568241786312
39	25.168741013453488	21.078660329148008	26.51144426840226	27.241154388996243
40	24.693935906398472	22.098382141792314	26.887340124694852	26.320341827114362
41	22.95030420700119	21.50838642582468	27.980429807503466	27.56087955967067
42	21.88112776897567	21.7902574387999	28.951205787208757	27.377409005015675
43	21.812847823233295	22.724603879644132	28.081272469546224	27.381275827576346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	22.0
2	27.0
3	71.0
4	115.0
5	115.0
6	145.5
7	176.0
8	201.0
9	226.0
10	360.5
11	495.0
12	495.0
13	1017.5
14	1540.0
15	2959.5
16	4379.0
17	4238.0
18	4097.0
19	4097.0
20	4763.0
21	5429.0
22	4519.5
23	3610.0
24	3805.0
25	4000.0
26	4000.0
27	4352.0
28	4704.0
29	5553.0
30	6402.0
31	7557.5
32	8713.0
33	8713.0
34	11019.5
35	13326.0
36	14482.5
37	15639.0
38	18518.5
39	21398.0
40	21398.0
41	25804.0
42	30210.0
43	36114.5
44	42019.0
45	59641.5
46	77264.0
47	77264.0
48	85656.5
49	94049.0
50	103859.0
51	113669.0
52	111480.5
53	109292.0
54	109292.0
55	97419.0
56	85546.0
57	81401.5
58	77257.0
59	73614.5
60	69972.0
61	69972.0
62	64556.5
63	59141.0
64	50559.0
65	41977.0
66	36487.5
67	30998.0
68	30998.0
69	27789.0
70	24580.0
71	20081.5
72	15583.0
73	11148.0
74	6713.0
75	6713.0
76	5379.0
77	4045.0
78	3316.0
79	2587.0
80	2078.0
81	1569.0
82	1569.0
83	1292.0
84	1015.0
85	768.5
86	522.0
87	392.0
88	262.0
89	262.0
90	183.0
91	104.0
92	71.0
93	38.0
94	24.5
95	11.0
96	11.0
97	6.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	982719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.305944841819695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.16520129991844	35.59135052178721
2	6.511892384881279	5.379597353315437
3	2.1065042505737055	2.610334451497686
4	1.0769193752007225	1.7793268924451184
5	0.6710567888172178	1.3859317352306322
6	0.4910328015995499	1.2169544291037118
7	0.36457838981771507	1.0541478402230982
8	0.28419593948297434	0.939118544044229
9	0.22247114844915636	0.827044288806342
>10	1.6429349844357048	13.689826924882095
>50	0.22541385664384234	6.518980014985167
>100	0.20708310440672745	17.2944610924038
>500	0.022045928578994536	6.11686161076368
>1k	0.008669747193986615	5.5960643005118165
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2573	0.2618245907527991	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2459	0.2502241230707863	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2356	0.2397429987615992	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2309	0.23496034980497985	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2138	0.21755964828196056	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2109	0.21460865211723798	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2058	0.20941896920686381	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2054	0.209011935253109	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2005	0.20402576931961222	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1897	0.1930358525682316	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1893	0.19262881861447678	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1868	0.19008485640350906	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1810	0.1841828640740639	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1636	0.16647688708572847	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1517	0.15436762696152206	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1350	0.1373739593922576	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1335	0.13584758206567696	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1329	0.1352370311350447	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1312	0.13350713683158666	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1297	0.131980759505006	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1263	0.1285209708980899	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1260	0.12821569543277378	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1260	0.12821569543277378	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1251	0.12729986903682539	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1240	0.12618052566399957	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1222	0.1243488728721028	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1191	0.12119435973050281	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	1188	0.12088908426518669	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1148	0.11681874472763833	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1136	0.11559764286637382	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1077	0.10959389204848996	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1070	0.108881582629419	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1045	0.10633762041845127	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1036	0.10542179402250287	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1001	0.10186024692714804	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.0175848843870933E-4	0.0	0.0	0.0	0.0
8	2.0351697687741866E-4	0.0	0.0	1.0175848843870933E-4	0.0
9	2.0351697687741866E-4	0.0	0.0	1.0175848843870933E-4	0.0
10	2.0351697687741866E-4	0.0	0.0	2.0351697687741866E-4	0.0
11	2.0351697687741866E-4	0.0	0.0	2.0351697687741866E-4	0.0
12	2.0351697687741866E-4	0.0	0.0	2.0351697687741866E-4	0.0
13	3.05275465316128E-4	0.0	0.0	2.0351697687741866E-4	0.0
14	3.05275465316128E-4	0.0	0.0	3.05275465316128E-4	0.0
15	4.070339537548373E-4	0.0	0.0	5.087924421935466E-4	0.0
16	5.087924421935466E-4	0.0	0.0	0.0010175848843870933	0.0
17	5.087924421935466E-4	0.0	0.0	0.001221101861264512	0.0
18	5.087924421935466E-4	0.0	0.0	0.0013228603497032214	0.0
19	5.087924421935466E-4	0.0	0.0	0.0015263773265806401	0.0
20	5.087924421935466E-4	0.0	0.0	0.002442203722529024	0.0
21	5.087924421935466E-4	0.0	0.0	0.003968581049109664	0.0
22	5.087924421935466E-4	0.0	0.0	0.005800233841006432	0.0
23	5.087924421935466E-4	0.0	0.0	0.006614301748516107	0.0
24	5.087924421935466E-4	0.0	0.0	0.00936178093636126	0.0
25	6.10550930632256E-4	0.0	0.0	0.010481124309187061	0.0
26	6.10550930632256E-4	0.0	0.0	0.01363563745078705	0.0
27	6.10550930632256E-4	0.0	0.0	0.03062930502005151	0.0
28	8.140679075096746E-4	0.0	0.0	0.11803984658890283	0.0
29	8.140679075096746E-4	0.0	0.0	0.2484942287673282	0.0
30	8.140679075096746E-4	0.0	0.0	0.4090691235236115	0.0
31	9.15826395948384E-4	0.0	0.0	0.8318756429864488	1.0175848843870933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1595	0.0	29.11285	1
AGTTCCG	55	1.9022946E-5	23.545454	32
GTTCTAC	65	2.682189E-6	22.76923	1
CCGCTTA	140	0.0	22.464285	25
ATACGGC	100	5.347829E-10	22.2	29
TTTTACG	80	6.957998E-7	20.8125	4
GCGCGAA	45	0.0038254198	20.555555	11
ATAGACT	55	5.142364E-4	20.181818	4
TAGCGAA	65	6.900829E-5	19.923077	10
TCGGGTA	65	6.900829E-5	19.923077	25
GCGGTAA	695	0.0	19.165468	23
GTATCAA	2500	0.0	18.574	2
GTAAGTT	80	1.616282E-5	18.5	29
CGCGGTA	735	0.0	18.37415	22
CTTATAC	900	0.0	18.294445	37
TACGGCT	125	8.572897E-9	17.76	30
GCAGTCG	420	0.0	17.61905	9
TATACAC	305	0.0	17.590164	37
CTTTATT	340	0.0	17.411764	2
CCCCTAT	75	2.0668822E-4	17.266666	1
>>END_MODULE