FastQCFastQC Report
Fri 10 Feb 2017
ERR1631848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608180
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46760.2907634717506747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44010.27366339588851996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37280.231814846596774No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC30600.19027720777524904No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT22260.13841734134238703No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17010.10577174196918254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.029.251311
ACTCTAT400.00193152123.1251
CTAGATA759.269543E-619.7333343
CTTATAC21850.019.55835237
GCGGTAA5750.018.33912823
TAGATAA1156.409573E-817.6956524
GTATCAA31650.017.5355472
GTACTGG5450.017.3119261
CCAGGAC8800.017.2386363
ACAGGCT7700.017.0584418
GACAGGC7700.016.8181827
ATGCTAG2650.016.75471715
TATTAGA3150.016.4444452
AGCCGCG6850.016.2043819
CGGTAAT6650.015.85714324
ATGATAC700.00259309715.8571423
TTATACT1403.475543E-815.8571424
GTATAGA700.00259309715.8571421
AAACACG1302.590641E-715.65384615
TGCGGGT6150.015.64227621
Produced by FastQC (version 0.11.5)