##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631848.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1608180
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.630112922682784	34.0	31.0	34.0	31.0	34.0
2	32.8013791988459	34.0	31.0	34.0	31.0	34.0
3	32.889189021129475	34.0	31.0	34.0	31.0	34.0
4	36.306042855899214	37.0	37.0	37.0	35.0	37.0
5	36.229381039435886	37.0	35.0	37.0	35.0	37.0
6	36.277694039224464	37.0	37.0	37.0	35.0	37.0
7	36.25986767650387	37.0	37.0	37.0	35.0	37.0
8	36.24016776728973	37.0	37.0	37.0	35.0	37.0
9	38.07141240408412	39.0	38.0	39.0	37.0	39.0
10	38.01107898369585	39.0	38.0	39.0	35.0	39.0
11	38.0804406223184	39.0	38.0	39.0	37.0	39.0
12	38.0284756681466	39.0	38.0	39.0	35.0	39.0
13	38.06644156748623	39.0	38.0	39.0	37.0	39.0
14	39.48345334477484	41.0	39.0	41.0	37.0	41.0
15	39.47795396037757	41.0	39.0	41.0	37.0	41.0
16	39.442503326742035	41.0	39.0	41.0	37.0	41.0
17	39.406988645549625	40.0	39.0	41.0	37.0	41.0
18	39.40626173687025	41.0	39.0	41.0	37.0	41.0
19	39.44152458058178	41.0	39.0	41.0	37.0	41.0
20	39.411933987488965	41.0	39.0	41.0	37.0	41.0
21	39.39024362944446	41.0	39.0	41.0	36.0	41.0
22	39.36264597246577	40.0	39.0	41.0	36.0	41.0
23	39.30160305438446	40.0	39.0	41.0	36.0	41.0
24	39.31569786964146	41.0	39.0	41.0	36.0	41.0
25	39.25446218706861	40.0	39.0	41.0	36.0	41.0
26	39.187579126714674	40.0	39.0	41.0	36.0	41.0
27	39.095575744008755	40.0	39.0	41.0	35.0	41.0
28	39.0757726125185	40.0	39.0	41.0	35.0	41.0
29	39.04609807359873	40.0	39.0	41.0	35.0	41.0
30	38.99121304829061	40.0	39.0	41.0	35.0	41.0
31	38.90929684488055	40.0	38.0	41.0	35.0	41.0
32	38.85178027335249	40.0	38.0	41.0	35.0	41.0
33	38.78974430722929	40.0	38.0	41.0	35.0	41.0
34	38.740130458033306	40.0	38.0	41.0	35.0	41.0
35	38.65475693019438	40.0	38.0	41.0	35.0	41.0
36	38.615343431705405	40.0	38.0	41.0	35.0	41.0
37	38.566625626484594	40.0	38.0	41.0	35.0	41.0
38	38.52849059184917	40.0	38.0	41.0	35.0	41.0
39	38.45706264224154	40.0	38.0	41.0	35.0	41.0
40	38.127698391971045	40.0	38.0	41.0	34.0	41.0
41	38.177396186993995	40.0	37.0	41.0	34.0	41.0
42	38.150220124612915	40.0	37.0	41.0	34.0	41.0
43	37.68217425910035	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	0.0
14	1.0
15	0.0
16	1.0
17	4.0
18	10.0
19	17.0
20	50.0
21	107.0
22	243.0
23	429.0
24	739.0
25	1389.0
26	2158.0
27	3476.0
28	5457.0
29	8013.0
30	11188.0
31	15244.0
32	20590.0
33	27521.0
34	39906.0
35	62710.0
36	93639.0
37	167242.0
38	365102.0
39	782939.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.83264311208944	17.07308883333955	12.647713564401993	27.44655449016901
2	19.651655909164397	19.3525600368118	34.776455371911105	26.219328682112696
3	20.694947083037967	21.01605541668221	29.075663172032982	29.213334328246837
4	15.371662376102178	15.729520327326544	33.6778221343382	35.22099516223308
5	16.551256700120636	33.79472447114129	33.0694947083038	16.58452412043428
6	34.48314243430462	33.80212414033255	15.350893556691414	16.363839868671416
7	30.183810269994655	28.430399582136328	20.575246552002884	20.810543595866136
8	26.9846658956087	32.48778121852032	19.666641793829047	20.86091109204194
9	27.748572920941683	13.92443631931749	18.56328271710878	39.76370804263205
10	19.24119190637864	24.519208048850253	30.55839520451691	25.681204840254203
11	37.03204865127038	20.780820554913007	20.13891479809474	22.048215995721872
12	23.433757415214714	24.289507393450982	27.0924274645873	25.184307726747008
13	32.10890571950901	18.614582944695247	22.74005397405763	26.536457361738115
14	23.547861557785822	20.109129575047568	24.410700294743126	31.932308572423484
15	27.857018492954772	24.826201047146466	21.852031489012425	25.46474897088634
16	25.254262582546733	24.705070327948363	22.995311470109066	27.04535561939584
17	25.35238592694848	24.806613687522542	23.6970985834919	26.143901802037085
18	24.98743921700307	22.986979069507143	25.31196756581975	26.71361414767004
19	26.795197055056025	23.104130134686415	24.92581676180527	25.174856048452288
20	26.740663358579265	22.899488863186956	25.063052643360816	25.296795134872962
21	26.763919461751794	23.430586128418458	23.95391063189444	25.851583777935303
22	26.762116181024513	24.2083597607233	23.38065390690097	25.648870151351215
23	26.335360469599173	23.899190389135544	24.16346428882339	25.601984852441888
24	26.740228083920954	23.5614794363815	23.75088609484013	25.94740638485742
25	26.21485157134152	23.24354238953351	24.770796801353082	25.770809237771893
26	26.398164384583815	23.852429454414306	24.518399681627677	25.2310064793742
27	26.593913616634957	23.375679339377434	24.290626671143777	25.739780372843835
28	25.179457523411557	23.624158987178053	24.796167095723114	26.400216393687277
29	26.012635401509783	23.952356079543335	24.630140904625105	25.40486761432178
30	25.34909027596413	23.26176174308846	25.512193908642068	25.87695407230534
31	25.573443271275604	23.59673668370456	24.85461826412466	25.975201780895173
32	24.18137273190812	23.461925903816738	25.270119016528003	27.08658234774714
33	24.95927072840105	23.41622206469425	25.803952293897446	25.82055491300725
34	25.26775609695432	23.205300401696327	25.788158042010224	25.73878545933913
35	25.20955365692895	23.72365033764877	25.677473914611546	25.38932209081073
36	25.254324764640774	22.855650486885796	25.66901714982154	26.22100759865189
37	24.4736907560099	22.49835217450783	26.154845850588863	26.87311121889341
38	24.813453717867404	21.84027907323807	27.053998930467984	26.292268278426544
39	23.543197900732505	21.851782760636247	27.96390951261675	26.641109826014503
40	23.70822917832581	22.056237485853575	28.6118469325573	25.623686403263317
41	22.434802074394657	21.627740675795	29.331853399495085	26.605603850315262
42	21.483789128082677	22.678369336765787	29.06894750587621	26.76889402927533
43	21.27031799922894	22.021788605753088	29.434826947232274	27.273066447785695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	126.0
2	105.0
3	247.5
4	390.0
5	390.0
6	497.0
7	604.0
8	636.0
9	668.0
10	1013.0
11	1358.0
12	1358.0
13	2561.0
14	3764.0
15	6444.0
16	9124.0
17	8980.0
18	8836.0
19	8836.0
20	9802.5
21	10769.0
22	9422.0
23	8075.0
24	8676.5
25	9278.0
26	9278.0
27	10380.0
28	11482.0
29	14782.5
30	18083.0
31	21572.0
32	25061.0
33	25061.0
34	31220.0
35	37379.0
36	42383.5
37	47388.0
38	56145.5
39	64903.0
40	64903.0
41	71681.5
42	78460.0
43	87318.0
44	96176.0
45	104001.0
46	111826.0
47	111826.0
48	117884.5
49	123943.0
50	137206.0
51	150469.0
52	151294.5
53	152120.0
54	152120.0
55	143076.5
56	134033.0
57	122252.5
58	110472.0
59	101465.0
60	92458.0
61	92458.0
62	88445.5
63	84433.0
64	68928.0
65	53423.0
66	47332.5
67	41242.0
68	41242.0
69	36037.0
70	30832.0
71	28280.5
72	25729.0
73	26923.0
74	28117.0
75	28117.0
76	24386.0
77	20655.0
78	14502.5
79	8350.0
80	5791.0
81	3232.0
82	3232.0
83	2647.0
84	2062.0
85	1710.5
86	1359.0
87	1091.5
88	824.0
89	824.0
90	618.5
91	413.0
92	259.5
93	106.0
94	67.5
95	29.0
96	29.0
97	16.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1608180.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.065333897089396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.79709749537075	38.66474846030015
2	6.811467094410685	6.1392207787730975
3	2.236207884267761	3.0232636490349147
4	1.1389807335967985	2.053141882475662
5	0.7116614443952836	1.6035630306679185
6	0.48211031664574433	1.303587743692318
7	0.3446845902040125	1.0873328302707674
8	0.2801475293902323	1.0099953561932384
9	0.20812886407351078	0.8441457077785227
>10	1.589531680769245	14.506221582024034
>50	0.21405279631512747	6.698117039276519
>100	0.16554806054819227	15.152757312540544
>500	0.01663796735720371	5.089737188658427
>1k	0.0037435426553708347	2.8241674383138826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4676	0.2907634717506747	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4401	0.27366339588851996	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3728	0.231814846596774	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3060	0.19027720777524904	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	2226	0.13841734134238703	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1701	0.10577174196918254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.218209404419903E-5
7	0.0	0.0	0.0	0.0	6.218209404419903E-5
8	0.0	0.0	0.0	0.0	6.218209404419903E-5
9	0.0	0.0	0.0	0.0	6.218209404419903E-5
10	0.0	0.0	0.0	0.0	6.218209404419903E-5
11	0.0	0.0	0.0	0.0	6.218209404419903E-5
12	0.0	0.0	0.0	1.2436418808839806E-4	6.218209404419903E-5
13	0.0	0.0	0.0	1.2436418808839806E-4	6.218209404419903E-5
14	0.0	0.0	0.0	1.865462821325971E-4	6.218209404419903E-5
15	6.218209404419903E-5	0.0	0.0	5.596388463977913E-4	6.218209404419903E-5
16	6.218209404419903E-5	0.0	0.0	8.705493166187865E-4	6.218209404419903E-5
17	1.2436418808839806E-4	0.0	0.0	0.0014923702570607768	6.218209404419903E-5
18	2.4872837617679613E-4	0.0	0.0	0.001803280727281772	6.218209404419903E-5
19	3.109104702209952E-4	0.0	0.0	0.0024251016677237624	6.218209404419903E-5
20	3.109104702209952E-4	0.0	0.0	0.0032334688902983496	1.2436418808839806E-4
21	3.730925642651942E-4	0.0	0.0	0.005036749617580122	1.2436418808839806E-4
22	3.730925642651942E-4	0.0	0.0	0.007586215473392282	1.2436418808839806E-4
23	3.730925642651942E-4	0.0	0.0	0.009140767824497258	1.2436418808839806E-4
24	3.730925642651942E-4	0.0	0.0	0.01218769043266301	1.2436418808839806E-4
25	3.730925642651942E-4	0.0	0.0	0.014301881630165778	1.2436418808839806E-4
26	3.730925642651942E-4	0.0	0.0	0.0194008133417901	1.2436418808839806E-4
27	3.730925642651942E-4	0.0	0.0	0.052481687373303984	1.2436418808839806E-4
28	3.730925642651942E-4	0.0	0.0	0.18405899837082915	1.2436418808839806E-4
29	3.730925642651942E-4	0.0	0.0	0.3664390802024649	1.2436418808839806E-4
30	3.730925642651942E-4	0.0	0.0	0.5976321058587969	1.865462821325971E-4
31	3.730925642651942E-4	0.0	0.0	1.2651568854232735	1.865462821325971E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1910	0.0	29.25131	1
ACTCTAT	40	0.001931521	23.125	1
CTAGATA	75	9.269543E-6	19.733334	3
CTTATAC	2185	0.0	19.558352	37
GCGGTAA	575	0.0	18.339128	23
TAGATAA	115	6.409573E-8	17.695652	4
GTATCAA	3165	0.0	17.535547	2
GTACTGG	545	0.0	17.311926	1
CCAGGAC	880	0.0	17.238636	3
ACAGGCT	770	0.0	17.058441	8
GACAGGC	770	0.0	16.818182	7
ATGCTAG	265	0.0	16.754717	15
TATTAGA	315	0.0	16.444445	2
AGCCGCG	685	0.0	16.20438	19
CGGTAAT	665	0.0	15.857143	24
ATGATAC	70	0.002593097	15.857142	3
TTATACT	140	3.475543E-8	15.857142	4
GTATAGA	70	0.002593097	15.857142	1
AAACACG	130	2.590641E-7	15.653846	15
TGCGGGT	615	0.0	15.642276	21
>>END_MODULE