FastQCFastQC Report
Fri 10 Feb 2017
ERR1631847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675219
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103350.6169342635201726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96560.5764022494969314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79670.47557961078521677No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA26680.1592627590780668No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.15460665142885796No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA23530.1404592474178003No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA22100.13192305006091742No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21610.1289980593582093No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21440.127983266665433No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18770.11204505202006423No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18350.1095379171320287No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT17340.10350885466318135No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16780.10016600814580065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32600.029.5092011
TAGTACT502.7028174E-422.24
GCAGTCG4250.021.3294129
GTACTAT951.6772537E-719.4736841
CAGTCGG4750.019.0842110
AGTCGGT4750.019.0842111
CAACCGT609.239218E-418.515
CCGCTTA1409.458745E-1118.525
TAAGATA801.6173899E-518.54
AGACGTA2150.018.06976725
GTCTAGA2400.017.7291681
GTATCAA54200.017.6808132
CTTATAC18650.017.65683637
CTCTATG7300.017.4863011
ATATGCC3850.017.298735
AACCGTC752.0679783E-417.26666616
GGTCGAT650.001580341817.07692311
TATACAC6200.017.00806437
TCTTATA34800.016.95833437
GATATGC3950.016.8607634
Produced by FastQC (version 0.11.5)