##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631847.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1675219
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64558544285852	34.0	31.0	34.0	31.0	34.0
2	32.806793619222326	34.0	31.0	34.0	31.0	34.0
3	32.897714269000055	34.0	31.0	34.0	31.0	34.0
4	36.314071772108605	37.0	37.0	37.0	35.0	37.0
5	36.23958718233258	37.0	35.0	37.0	35.0	37.0
6	36.289840910352616	37.0	37.0	37.0	35.0	37.0
7	36.27366272708225	37.0	37.0	37.0	35.0	37.0
8	36.26147148522074	37.0	37.0	37.0	35.0	37.0
9	38.08517931088413	39.0	38.0	39.0	37.0	39.0
10	38.02383031711078	39.0	38.0	39.0	35.0	39.0
11	38.10041254307646	39.0	38.0	39.0	37.0	39.0
12	38.034670690817144	39.0	38.0	39.0	35.0	39.0
13	38.086155899616706	39.0	38.0	39.0	37.0	39.0
14	39.46792747694481	41.0	39.0	41.0	37.0	41.0
15	39.459724370365905	41.0	39.0	41.0	37.0	41.0
16	39.422022434081754	40.0	39.0	41.0	37.0	41.0
17	39.409292158219316	40.0	39.0	41.0	37.0	41.0
18	39.438774273691976	41.0	39.0	41.0	37.0	41.0
19	39.464658650600306	41.0	39.0	41.0	37.0	41.0
20	39.444406970073764	41.0	39.0	41.0	37.0	41.0
21	39.41444133572983	41.0	39.0	41.0	37.0	41.0
22	39.38060934122643	41.0	39.0	41.0	36.0	41.0
23	39.30845161140125	40.0	39.0	41.0	36.0	41.0
24	39.31408788940431	41.0	39.0	41.0	36.0	41.0
25	39.25660943434858	40.0	39.0	41.0	36.0	41.0
26	39.18523548264436	40.0	39.0	41.0	36.0	41.0
27	39.0535589675141	40.0	39.0	41.0	35.0	41.0
28	39.03318013943252	40.0	39.0	41.0	35.0	41.0
29	38.98652832853496	40.0	39.0	41.0	35.0	41.0
30	38.91421599205835	40.0	38.0	41.0	35.0	41.0
31	38.78628943439634	40.0	38.0	41.0	35.0	41.0
32	38.698408387201916	40.0	38.0	41.0	35.0	41.0
33	38.61137857199566	40.0	38.0	41.0	35.0	41.0
34	38.559201513354374	40.0	38.0	41.0	35.0	41.0
35	38.4700895823173	40.0	38.0	41.0	35.0	41.0
36	38.38849189270179	40.0	38.0	41.0	35.0	41.0
37	38.32511271660601	40.0	38.0	41.0	35.0	41.0
38	38.264106961537564	40.0	38.0	41.0	34.0	41.0
39	38.17770154230581	40.0	37.0	41.0	34.0	41.0
40	37.830673482093985	40.0	37.0	41.0	33.0	41.0
41	37.84964950851202	40.0	37.0	41.0	33.0	41.0
42	37.785770696249266	40.0	37.0	41.0	33.0	41.0
43	37.3047100110493	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	1.0
13	1.0
14	0.0
15	1.0
16	4.0
17	5.0
18	10.0
19	26.0
20	70.0
21	132.0
22	285.0
23	548.0
24	933.0
25	1527.0
26	2495.0
27	4057.0
28	6247.0
29	9232.0
30	12948.0
31	17240.0
32	22788.0
33	30191.0
34	43958.0
35	65785.0
36	100652.0
37	191359.0
38	376878.0
39	787838.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.25428496214524	17.26126554199779	12.85193159819701	26.632517897659945
2	20.206910260688304	20.67132715185298	33.25314481270807	25.868617774750646
3	21.26366761599528	20.97283996898316	29.474355293248227	28.289137121773333
4	15.298775861544073	16.28718394430818	34.010777098397284	34.40326309575046
5	16.76055488864441	33.41234787809832	32.76085096933595	17.066246263921315
6	33.16778283913924	34.209736159869244	15.524656776218512	17.097824224773
7	29.287215581962716	29.063483640049448	20.402228007203835	21.247072770783998
8	27.36078088894646	31.27477661129679	20.11569830571406	21.24874419404269
9	28.015501256850595	13.419200713458956	18.98587587652719	39.57942215316326
10	19.13940804157546	24.572846893450944	30.213004986213743	26.074740078759852
11	37.25871065215951	20.56125199153066	19.927961657550448	22.252075698759384
12	22.86936812440642	25.14160835090815	27.375107374020953	24.61391615066448
13	34.13470119429161	18.571721070498842	22.248792545929817	25.04478518927973
14	23.73474751659335	20.826709821223375	25.11217936281764	30.32636329936563
15	28.101937716800013	25.33734395323835	21.864126421679792	24.696591908281842
16	24.23975611546908	24.74327237214955	24.065928096565283	26.951043415816084
17	25.109612534241794	24.37167916553	24.175764482136366	26.342943818091847
18	25.709951952550682	22.06326456421519	25.56471721010805	26.66206627312608
19	26.66642391233624	23.176372760815152	25.997615834108856	24.159587492739757
20	27.45623109575524	22.491686161630213	25.43195844841779	24.620124294196756
21	25.967112359637756	23.329964619551234	25.116238533588742	25.586684487222268
22	26.119032795115142	23.830257417089946	24.742376966832396	25.308332820962516
23	25.980185277268227	23.18049162527407	25.345641375843996	25.493681721613708
24	25.72266670805429	23.11464948761923	25.443240555413947	25.719443248912533
25	25.680343883396738	23.61130097020151	25.650616426867174	25.057738719534576
26	25.653660804945506	23.75026787542405	25.24332639493702	25.352744924693425
27	25.23831212516095	23.281612732424836	26.436603214266313	25.043471928147902
28	24.899848915276152	23.70866137502022	25.25001208797178	26.141477621731845
29	24.98318130345943	24.2634545095298	25.38760603837468	25.365758148636086
30	25.022041894223978	23.80369372601433	26.163982142036357	25.010282237725335
31	25.957262901149043	23.599899475829726	25.231268269999322	25.2115693530219
32	23.788949385125168	24.099834111241574	25.59337018025703	26.517846323376226
33	24.18955372402056	23.39049401899095	27.037539569453305	25.382412687535183
34	24.477277299266547	23.13052800857679	26.478926038923866	25.9132686532328
35	24.20895417255893	24.27181162582325	26.764799109847726	24.75443509177009
36	24.464502850075124	23.433831636341278	26.94602914604001	25.155636367543586
37	24.621437555328587	23.20627929840815	26.53945543836358	25.632827707899686
38	24.04043889187026	22.65656012736245	27.470915742956592	25.8320852378107
39	23.542892003970824	21.82347502028093	28.39557096713922	26.238062008609024
40	23.2336190074253	22.74001190292135	28.7321836727019	25.294185416951454
41	22.288429154635903	22.137344430787856	29.574163139267164	26.000063275309078
42	21.117059918733013	22.8692487370308	29.82242918687049	26.191262157365692
43	20.610678365037646	23.11614182981449	29.679701579315896	26.59347822583197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	236.5
2	237.0
3	514.5
4	792.0
5	792.0
6	1023.0
7	1254.0
8	1317.0
9	1380.0
10	2064.0
11	2748.0
12	2748.0
13	5167.5
14	7587.0
15	13016.0
16	18445.0
17	17676.5
18	16908.0
19	16908.0
20	18695.0
21	20482.0
22	15953.0
23	11424.0
24	11339.5
25	11255.0
26	11255.0
27	12120.5
28	12986.0
29	14891.0
30	16796.0
31	19368.5
32	21941.0
33	21941.0
34	27080.0
35	32219.0
36	36645.0
37	41071.0
38	45681.0
39	50291.0
40	50291.0
41	59106.0
42	67921.0
43	77360.5
44	86800.0
45	105249.5
46	123699.0
47	123699.0
48	134046.5
49	144394.0
50	157530.0
51	170666.0
52	168057.0
53	165448.0
54	165448.0
55	152290.0
56	139132.0
57	130862.5
58	122593.0
59	114280.5
60	105968.0
61	105968.0
62	97105.0
63	88242.0
64	72853.5
65	57465.0
66	49261.5
67	41058.0
68	41058.0
69	34771.5
70	28485.0
71	24543.0
72	20601.0
73	16486.0
74	12371.0
75	12371.0
76	10368.5
77	8366.0
78	7465.0
79	6564.0
80	6166.5
81	5769.0
82	5769.0
83	5334.0
84	4899.0
85	3923.0
86	2947.0
87	2636.5
88	2326.0
89	2326.0
90	1646.5
91	967.0
92	686.5
93	406.0
94	236.0
95	66.0
96	66.0
97	38.5
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1675219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.55360631604252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.39642004704159	37.6016487325925
2	7.4785065739520205	6.663888754555885
3	2.417279702438501	3.2309558465461614
4	1.2752638481305856	2.2727041375476618
5	0.8010443606836526	1.7844707543792717
6	0.5521727968817618	1.4760773646418877
7	0.3995550930204332	1.246113422120149
8	0.31047010309995277	1.1066050197133144
9	0.25899896084762053	1.0385403964082096
>10	1.7289268617504678	15.396474864818044
>50	0.21869519183995667	6.83662705783721
>100	0.14416037328129933	12.280194894584385
>500	0.012605595514664385	3.7876808923907177
>1k	0.005498185277672763	3.607602491733479
>5k	2.6820415988647626E-4	1.0529268927612843
>10k+	1.3410207994323813E-4	0.6174884773697936
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10335	0.6169342635201726	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9656	0.5764022494969314	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7967	0.47557961078521677	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2668	0.1592627590780668	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2590	0.15460665142885796	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2353	0.1404592474178003	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2210	0.13192305006091742	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2161	0.1289980593582093	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2144	0.127983266665433	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1877	0.11204505202006423	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1835	0.1095379171320287	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1734	0.10350885466318135	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1678	0.10016600814580065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.969368781036987E-5	0.0
8	1.790810634311096E-4	0.0	0.0	5.969368781036987E-5	0.0
9	1.790810634311096E-4	0.0	0.0	5.969368781036987E-5	0.0
10	1.790810634311096E-4	0.0	0.0	5.969368781036987E-5	0.0
11	1.790810634311096E-4	0.0	0.0	1.790810634311096E-4	0.0
12	2.3877475124147948E-4	0.0	0.0	2.984684390518493E-4	0.0
13	2.3877475124147948E-4	0.0	0.0	2.984684390518493E-4	0.0
14	2.984684390518493E-4	0.0	0.0	9.550990049659179E-4	0.0
15	3.581621268622192E-4	0.0	0.0	0.0011938737562073973	0.0
16	3.581621268622192E-4	0.0	0.0	0.0020295853855525755	5.969368781036987E-5
17	4.1785581467258906E-4	0.0	0.0	0.0029249907027081236	5.969368781036987E-5
18	4.1785581467258906E-4	0.0	0.0	0.003462233893001452	5.969368781036987E-5
19	4.7754950248295896E-4	0.0	0.0	0.004238251834536261	5.969368781036987E-5
20	5.372431902933288E-4	0.0	0.0	0.005312738215122919	5.969368781036987E-5
21	5.372431902933288E-4	0.0	0.0	0.008178035230020671	5.969368781036987E-5
22	5.372431902933288E-4	0.0	0.0	0.011282106996159905	5.969368781036987E-5
23	5.372431902933288E-4	0.0	0.0	0.013848935572005809	5.969368781036987E-5
24	5.372431902933288E-4	0.0	0.0	0.01719178208938652	5.969368781036987E-5
25	5.372431902933288E-4	0.0	0.0	0.01904228641150799	5.969368781036987E-5
26	5.372431902933288E-4	0.0	0.0	0.023280538246044247	5.969368781036987E-5
27	5.372431902933288E-4	0.0	0.0	0.046680463867709236	5.969368781036987E-5
28	5.372431902933288E-4	0.0	0.0	0.15717348000470385	5.969368781036987E-5
29	5.372431902933288E-4	0.0	0.0	0.3164362390827707	5.969368781036987E-5
30	5.372431902933288E-4	0.0	0.0	0.5189769218233556	5.969368781036987E-5
31	5.372431902933288E-4	0.0	0.0	1.0943046849397005	5.969368781036987E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3260	0.0	29.509201	1
TAGTACT	50	2.7028174E-4	22.2	4
GCAGTCG	425	0.0	21.329412	9
GTACTAT	95	1.6772537E-7	19.473684	1
CAGTCGG	475	0.0	19.08421	10
AGTCGGT	475	0.0	19.08421	11
CAACCGT	60	9.239218E-4	18.5	15
CCGCTTA	140	9.458745E-11	18.5	25
TAAGATA	80	1.6173899E-5	18.5	4
AGACGTA	215	0.0	18.069767	25
GTCTAGA	240	0.0	17.729168	1
GTATCAA	5420	0.0	17.680813	2
CTTATAC	1865	0.0	17.656836	37
CTCTATG	730	0.0	17.486301	1
ATATGCC	385	0.0	17.2987	35
AACCGTC	75	2.0679783E-4	17.266666	16
GGTCGAT	65	0.0015803418	17.076923	11
TATACAC	620	0.0	17.008064	37
TCTTATA	3480	0.0	16.958334	37
GATATGC	395	0.0	16.86076	34
>>END_MODULE