FastQCFastQC Report
Fri 10 Feb 2017
ERR1631846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395607
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16970.4289610648952117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16090.4067167668923957No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16000.40444178186938046No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15390.38902243893561034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.38497802111691654No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14960.37815306604787075No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14030.354644887476713No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13760.34781993240766723No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13530.3420060817932949No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12940.32709229108686144No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12340.3119257242667597No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11680.29524250076464775No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11550.29195641128695904No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11480.2901869784912805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11320.2861425606725867No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9070.22926793509720506No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8460.21384859216343494No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8390.2120791593677564No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8320.21030972657207783No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8240.20828751766273093No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8230.20803474154906257No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8180.20677086098072076No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7740.1956487119793128No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7630.1928681747289608No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7520.19008763747860882No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7310.1847793390915732No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7300.18452656297790485No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7120.17997659293187432No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7070.1787127123635325No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6880.17390996620383362No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6840.17289886174916017No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6810.17214053340815508No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6610.16708501113478783No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6490.16405169777076745No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT6470.16354614554343072No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC6410.16202948886142055No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6240.15773229492905838No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6230.15747951881539002No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6180.1562156382470482No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6160.15571008601971148No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6080.15368787711036458No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6020.1521712204283544No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5910.14939068317800241No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5800.14661014592765043No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5790.14635736981398206No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5790.14635736981398206No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5680.14357683256363007No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA5670.1433240564499617No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5640.14256572810895662No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5410.13675187749458428No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5390.13624632526724756No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5170.13068525076654355No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA4930.12461862403850285No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4850.12259641512915595No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4840.12234363901548759No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4820.12183808678815086No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4770.12057420621980905No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4760.12032143010614069No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4730.1195631017651356No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT4620.11678256451478361No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4550.11501313171910507No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4540.11476035560543671No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4490.1134964750370949No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4490.1134964750370949No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4470.11299092280975817No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4440.11223259446875308No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4380.11071593778674291No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA4360.11021038555940618No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT4340.10970483333206946No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG4340.10970483333206946No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4310.10894650499106437No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4290.10844095276372764No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC4210.10641874385438074No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4200.10616596774071238No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4170.10540763939970729No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4150.10490208717237055No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4120.10414375883136547No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4110.1038909827176971No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG4080.10313265437669202No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4080.10313265437669202No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4060.10262710214935529No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG4050.10237432603568693No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4050.10237432603568693No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4030.1018687738083502No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4020.10161599769468184No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC3960.10009934101267166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATGC401.5965579E-632.37532
AGCCCAC250.005492042329.5999987
CTCCACT250.005492042329.5999982
CACGAGA250.005492042329.59999811
TATGCCG250.005492042329.59999833
CGTAGTA250.005492042329.59999820
GTATGCC250.005492042329.59999832
AAACCCC250.005492042329.59999830
GACGCGT250.005492042329.59999816
GTAGTAA250.005492042329.59999821
ACGCGTA250.005492042329.59999817
GGTATCA11550.028.5108221
TTTTGCG405.930436E-527.752
GATAACC358.8590756E-426.4285734
TAAGTCT358.8590756E-426.4285735
ATACACA358.8590756E-426.4285737
TCTTTGT451.3210699E-424.66666837
GCACATA701.9163053E-723.78571331
CAATGCT551.8985727E-523.54545433
CTAAGTC400.001928961123.12534
Produced by FastQC (version 0.11.5)